Supplementation of fungal and/or bacterial phytase in broiler diets formulated with reduced phosphorus level and different calcium contents

Nardelli, Nicole Batelli de Souza; Naves, Luciana de Paula; Oliveira, Diego Humberto de; García, Adriana; Lima, Eduardo Machado Costa; Oliveira, Eduardo Carvalho; Rodrigues, Paulo Borges

doi:10.1590/rbz4720170297

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…However, the concentration levels and rations for poultry must be close to their specified requirements. Consequently, a study on the effect of reducing dietary Ca levels and calcium, along with available phosphorus (Ca:aP) ratios in combination supplemented fungal phytases on poultry growth performance, nutrient digestibility, bone ash, and mineralization, was conducted (Delezie et al, 2015;de Souza Nardelli et al, 2018;Ajith et al, 2019).…”

Section: Poultrymentioning

confidence: 99%

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases

et al. 2020

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Phytases are a group of enzymes that hydrolyze the phospho-monoester bonds of phytates. Phytates are one of the major forms of phosphorus found in plant tissues. Fungi are mainly used for phytase production. The production of fungal phytases has been achieved under three different fermentation methods including solid-state, semi-solid-state, and submerged fermentation. Agricultural residues and other waste materials have been used as substrates for the evaluation of enzyme production in the fermentation process. Nutrients, physical conditions such as pH and temperature, and protease resistance are important factors for increasing phytase production. Fungal phytases are considered monomeric proteins and generally possess a molecular weight of between 14 and 353 kDa. Fungal phytases display a broad substrate specificity with optimal pH and temperature ranges between 1.3 and 8.0 and 37-67 • C, respectively. The crystal structure of phytase has been studied in Aspergillus. Notably, thermostability engineering has been used to improve relevant enzyme properties. Furthermore, fungal phytases are widely used in food and animal feed additives to improve the efficiency of phosphorus intake and reduce the amount of phosphorus in the environment.

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Section: Poultrymentioning

confidence: 99%

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases

et al. 2020

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“…The supplementation of phytase in animal feed can reduce the antinutritional effect of phytate (Walk & Rama Rao, 2020). Several commercially available fungal phytases such as Allzyme SSF, Finase PL, and Natuphos from Aspergillus niger, Phyzyme and Ronozyme from Aspergillus oryzae, and Finase from Trichoderma reesei have been used as feed supplements but having limited efficacy (Nardelli et al, 2018;Wodzinski & Ullah, 1996). This is why the focus has shifted toward bacterial phytases, which can be better alternatives to fungal counterparts due to their high substrate specificity, greater tolerance to proteolysis, high thermal stability, better pH optima, and the catalytic efficiency (Jorquera et al, 2008).…”

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confidence: 99%

Purification and characterization of phytase from Bacillus subtilis P6: Evaluation for probiotic potential for possible application in animal feed

2021

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The antinutritional property of phytate is the main problem associated with food animals, but phytase-producing probiotic strain could be the best alternative for resolving this problem. In the present study, phytase from probiotic strain Bacillus subtilis P6 was purified to homogeneity, which showed a relative molecular mass of 40 kDa on native and SDS-PAGE. The purification fold of purified phytase was 86.99, with a specific activity of 104.39 U/mg protein. The purified enzyme showed optimum activity at 40 • C and pH 6.0. Semi-metal ions, such as K + and Ca 2+ , slightly enhance the enzyme activity while Cu 2+ , Co 2+ , and Zn 2+ prominently inhibited phytase activity. Purified phytase showed the highest substrate specificity toward its natural substrate phytate and did not hydrolyze other substrates having a phosphate bond. The purified enzyme exhibited strong proteolytic tolerance against trypsin and pepsin. Further, we evaluated the probiotic potential of B. subtilis and noted that it showed excellent resistance toward high acidic and bile concentrations. Subsequently, it revealed exceptional antimicrobial activity, good hydrophobicity, autoaggregation ability, nonhemolytic, and nonlecithinase activity. Additionally, B. subtilis P6 displayed susceptibility against major antibiotics. Owing to the excellent biochemical properties of phytase with notable probiotic characteristics, B. subtilis P6 may offer the possibility of being a promising candidate for the application in animal feed and food supplements. However, the in vivo use of strain secreting phytase needs to be studied in animals for its intended benefits

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Evaluating the inclusion of phytase sources to phosphorus-free diets for GIFT tilapia (Oreochromis niloticus): Growth performance, intestinal morphometry, immune-antioxidant responses, and phosphorus utilization

Akpoilih

Adeshina

Chukwudi

et al. 2023

Animal Feed Science and Technology

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Supplementation of fungal and/or bacterial phytase in broiler diets formulated with reduced phosphorus level and different calcium contents

Cited by 4 publications

References 40 publications

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases

Purification and characterization of phytase from Bacillus subtilis P6: Evaluation for probiotic potential for possible application in animal feed

Evaluating the inclusion of phytase sources to phosphorus-free diets for GIFT tilapia (Oreochromis niloticus): Growth performance, intestinal morphometry, immune-antioxidant responses, and phosphorus utilization

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