Use of a mixture of vegetal (Jatropha curcas) and animal (fish silage) byproducts as protein source in shrimp (Litopenaeus vannamei) diets

Abstract

This study evaluated growth responses of white shrimp Litopenaeus vannamei by testing different combinations of Jatropha curcas kernel meal (JCK) and tilapia waste silage (FS) as dietary protein sources under laboratory and pond conditions. A reference diet (RD) based on fish meal was elaborated to contain 35% crude protein and 434 kcal/g; then, five isoproteic and isoenergetic experimental diets were formulated from RD with different FS/JCK proportions as follows: 75%FS/0%JCK; 56.25%FS/13.25%JCK; 26.5%FS/30.89%JCK; 18.75%FS/39.75%JCK; and 0%FS/53%JCK. For the laboratory trial, shrimp (2.90 g) were stocked at 10 m 2 in an indoor recirculating system during 90 days. There were differences among the growth parameters of diets. Mean weight gain fluctuated from 6.71±0.61 g observed for the shrimp fed the 75%FS/ 0%JCK diet, to 9.70±0.61 g for the group fed the RD. The RD and 18.75%FS/39.75%JCK diet obtained the highest mean final weight (above 12 g), weight gain (above 9 g), and specific growth rate values (above 1.6%/day). Average final survival was 90.97%. The RD and 18.75%FS/39.75%JCK diets were used for the pond trial. Shrimp (0.49 g) were stocked at 10 m 2 within 1-m 3 cages into the pond for 35 days. Weight gain (3.47 g) and survival (93.33%) of shrimps were similar between the diets. Quadratic equations with the laboratory data indicated that the maximum responses for growth parameters correspond to FS and JKC inclusion levels in the range of 27.83 to 29.00%, and 32.25 to 33.64%, respectively. A combination of 18.75% FS/39.75%JCK meals as protein source is a potential alternative to effectively substitute FM in practical diets for L. vannamei at the studied ages under both laboratory and pond conditions.

Dietary Different Replacement Levels of Fishmeal by Fish Silage Could Influence Growth of Litopenaeus vannamei by Regulating mTOR at Transcriptional Level

“…Many researchers also focused on the effects of dietary FS in shrimp. Rodríguez-González et al (2018) found that a combination of 18.75% FS/39.75% Jatropha curcas kernel meals in shrimp diets made better growth performance. And in a biofloc system, up to 6% of tilapia silage inclusion did not compromise shrimp meat quality (Gonçalves et al, 2019).…”

“…And FS has been successfully used as a substitute of fishmeal in several fish species (Liang et al, 2006;Goosen et al, 2016). Recently, more and more studies were conducted to evaluate the effect of dietary FS in shrimp at different nutrient combination conditions (Gallardo et al, 2012;Rodríguez-González et al, 2018) and biofloc system (Gonçalves et al, 2019;Lobato et al, 2019). Here, we examined the performances of replacement of fishmeal in L. vannamei diet with FS.…”