Curcumin Mediated Gold Nanoparticles and Analysis of its Antioxidant, Anti-inflammatory, Antimicrobial Activity Against Oral Pathogens

Abstract

Objective: To green synthesise gold nanoparticles using curcumin and to analyse its antioxidant, antiinflammatory, and antimicrobial activity among oral pathogens. Material and Methods: Biosynthesised Curcumin Gold nanoparticles (CuAuNP) were evaluated by UV-visible spectrophotometer (UV-Vis), Transmission Electron Microscopy (TEM), and evaluation of antioxidant, anti-inflammatory and antibacterial activity against oral pathogens. Results: Synthesized CuAuNP were characterized using UVvisible spectrophotometry and showed peak absorption at 530nm. CuAuNp showed a 90.3% maximum scavenging ability of DPPH at a concentration of 50 μg/mL. CuAuNP exhibited 79.6 % of the highest antiinflammatory activity at 50μg/mL than the standard drug diclofenac. TEM image clearly showed uniformly dispersed spherical-shaped gold nanoparticles with a size of about 20 nm. The biosynthesized nanoparticle was tested for its antimicrobial effect, and it showed a potent effect against S. aureus, E. faecalis, and C. albicans at 100µg/ mL. Enterococcus faecalis has a maximum zone of inhibition of 14 mm at 100µg/ mL of CuAuNp. Among gram-positive bacteria, a maximum zone of inhibition of 12 mm at 100µg/ mL was seen in S. aureus compared to S mutans. Candida albicans showed a maximum zone of inhibition of 18 mm at 25 μg/mL of CuAuNp. Conclusion: Curcumin-mediated gold nanoparticles with 20 nm size were effective and had strong antioxidant and anti-inflammatory activity at 50µg/ mL, antimicrobial action inhibiting microbes at 100µg/ mL concentration that can be used in treating various Oral mucosal lesions.

Green Synthesis of Selenium, Zinc Oxide, and Strontium Nanoparticles and Their Antioxidant Activity - A Comparative In Vitro Study

“…In the final analysis, the demonstrated antioxidant potential of selenium nanoparticles, zinc oxide nanoparticles, and strontium nanoparticles in these studies have drawn the versatility and applicability of green-synthesized nanoparticles in effectively managing oxidative stress. These findings not only enhance our understanding of the therapeutic applications of nanoparticles but also pave the way for further research and development in the field of nanomedicine [20,21] and antioxidant [22] therapy. The environmentally friendly and cost-effective green synthesis approach [23], utilizing medicinal plants, adds a sustainable dimension to the production of these nanoparticles, making them promising evidence for various biomedical applications.…”