Cytotoxicity, Morphology and Chemical Composition of Two Luting Cements: An in Vitro Study

Said, Farrukh; Moeen, Faisal; Khan, Muhammad Talal; Mansoor, Afsheen; Uzbek, Usman Haider; Alam, Mohammad Khursheed; Siddiqui, Ammar Ahmed

doi:10.1590/pboci.2020.041

Cited by 7 publications

(7 citation statements)

References 19 publications

(23 reference statements)

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“…Thereafter, 100 µL of cell suspension was obtained and seeded in each well of the standard 96-well plate for 24–48 h. The TiO 2 nanoparticles were added as 1 mg/mL stock solution concentration to test cytotoxicity. MTT dye (Sigma Aldrich, Saint Louis, MO, USA) was added to each well, and the plates were incubated at 37 °C for 2 h. The fluorescence of each well was measured at a wavelength of 490 nm with a fluorescence well plate reader (Thermo Fisher, Waltham, MA, USA) at day-1, day-3, day-5, day-7, day-21 and day-30 [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Titanium Oxide Nanoparticles with a Novel Biogenic Process for Dental Application

Mansoor

Khan²,

Mehmood

et al. 2022

Nanomaterials

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The prevalence of dental caries has been largely consonant over time despite the enhancement in dental technologies. This study aims to produce novel GIC restorative material by incorporating TiO2 nanoparticles synthesized by Bacillus subtilis for the treatment of dental caries. The TiO2 nanoparticles were prepared by inoculating a fresh culture of Bacillus subtilis into a nutrient broth for 24 h, which was then characterized by XRD, DRS, FTIR, AFM, SEM, TEM and EDX. These TiO2 nanoparticles were incorporated in GIC restorative material at different concentrations (0–10% TiO2 -GIC) and were tested for their mechanical properties in a universal testing machine. The XRD analysis revealed synthesis of anatase and rutile-phased TiO2 nanoparticles with a particle size of 70.17 nm that was further confirmed by SEM and TEM analysis. The EDX spectrum indicated prominent peaks of titanium and oxygen with no impurities in the prepared material. Treatment with 5% TiO2 -GIC proved to be most effective for the treatment of dental caries with no observable cytotoxic effect. An increase in the compressive strength of TiO2 nanoparticle-reinforced GIC was observed as the concentration of the TiO2 nanoparticles was increased up to 5%; subsequently, the compressive strength was lowered. An increase in the flexural strength was observed in GIC containing 0%, 3% and 5% TiO2 nanoparticles sequentially. Based on the results, it can be concluded that Bacillus subtilis-derived TiO2 nanoparticles have excellent potential for developing next generation of restorative materials for dental issues.

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Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Titanium Oxide Nanoparticles with a Novel Biogenic Process for Dental Application

Mansoor

Khan²,

Mehmood

et al. 2022

Nanomaterials

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“…Therefore, even though newer sealers have been introduced that have been deemed highly biocompatible as a result, there is still a concern about their cytotoxicity. There have been numerous studies examining dental materials' cytotoxicity, especially soft tissues [ 17 , 18 ]. In this review article, therapeutic applications of herbal and chemical materials in oral biofilm therapy in in vitro , in vivo , and clinical studies are discussed in the recent decade (2010-2022).…”

Section: Introductionmentioning

confidence: 99%

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

Moghaddam

Ranjbar

Yazdanian

et al. 2022

BioMed Research International

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Herbal and chemical products are used for oral care and biofilm treatment and also have been reported to be controversial in the massive trials conducted in this regard. The present review is aimed at evaluating the potential of relevant herbal and chemical products and comparing their outcomes to conventional oral care products and summarizing the current state of evidence of the antibiofilm properties of different products by evaluating studies from the past eleven years. Chlorhexidine gluconate (CHX), essential oils (EOs), and acetylpyridinium chloride were, respectively, the most commonly studied agents in the included studies. As confirmed by all systematic reviews, CHX and EO significantly control the plaque formation and gingival indices. Fluoride is another interesting reagent in oral care products that has shown promising results of oral health improvement, but the evidence quality needs to be refined. The synergy between natural plants and chemical products should be targeted in the future to accede to the formation of new, efficient, and healthy anticaries strategies. Moreover, to discover their biofilm-interfering or biofilm-inhibiting activities, effective clinical trials are needed. In this review article, therapeutic applications of herbal/chemical materials in oral biofilm infections are discussed in recent years (2010-2022).

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“…Later on, the fluorescence reader ‘BIORAD’ (Thermo-Fisher, New York, NY, USA) was utilized to measure fluorescence at 490 nm wavelength for day 1. Similarly, the readings were obtained in triplicates for the remaining days, i.e., day 15, day 31, day 41 and day 51, with the reader [ 36 ]. The cytotoxicity (cell viability) was measured as [ 37 ]:

…”

Section: Materials and Methodologymentioning

confidence: 99%

Effect of Currently Available Nanoparticle Synthesis Routes on Their Biocompatibility with Fibroblast Cell Lines

et al. 2022

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Nanotechnology has acquired significance in dental applications, but its safety regarding human health is still questionable due to the chemicals utilized during various synthesis procedures. Titanium nanoparticles were produced by three novel routes, including Bacillus subtilis, Cassia fistula and hydrothermal heating, and then characterized for shape, phase state, size, surface roughness, elemental composition, texture and morphology by SEM, TEM, XRD, AFM, DRS, DLS and FTIR. These novel titanium nanoparticles were tested for cytotoxicity through the MTT assay. L929 mouse fibroblast cells were used to test the cytotoxicity of the prepared titanium nanoparticles. Cell suspension of 10% DMEM with 1 × 104 cells was seeded in a 96-well plate and incubated. Titanium nanoparticles were used in a 1 mg/mL concentration. Control (water) and titanium nanoparticles stock solutions were prepared with 28 microliters of MTT dye and poured into each well, incubated at 37 °C for 2 h. Readings were recorded on day 1, day 15, day 31, day 41 and day 51. The results concluded that titanium nanoparticles produced by Bacillus subtilis remained non-cytotoxic because cell viability was >90%. Titanium nanoparticles produced by Cassia fistula revealed mild cytotoxicity on day 1, day 15 and day 31 because cell viability was 60–90%, while moderate cytotoxicity was found at day 41 and day 51, as cell viability was 30–60%. Titanium nanoparticles produced by hydrothermal heating depicted mild cytotoxicity on day 1 and day 15; moderate cytotoxicity on day 31; and severe cytotoxicity on day 41 and day 51 because cell viability was less than 30% (p < 0.001). The current study concluded that novel titanium nanoparticles prepared by Bacillus subtilis were the safest, more sustainable and most biocompatible for future restorative nano-dentistry purposes.

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Cytotoxicity, Morphology and Chemical Composition of Two Luting Cements: An in Vitro Study

Cited by 7 publications

References 19 publications

Synthesis and Characterization of Titanium Oxide Nanoparticles with a Novel Biogenic Process for Dental Application

Synthesis and Characterization of Titanium Oxide Nanoparticles with a Novel Biogenic Process for Dental Application

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

Effect of Currently Available Nanoparticle Synthesis Routes on Their Biocompatibility with Fibroblast Cell Lines

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