Purification and the effects on structure and bioactivity for polysaccharide from Actinidia valvata Dunn. using macroporous adsorption resin

Pan, Feng; Li, Sanhua; Xin-ting, Zhu; YANG, Jianbo; Wen, Jing; Song, Changwei; Luo, Xiao; RUAN, Guoyong; Lei, Yang

doi:10.1590/fst.99721

Cited by 3 publications

(1 citation statement)

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“…Generally, patent procedures differ mainly in the choice of extraction agent, the method of breaking the heparin protein complex, and the final purification method. The ion exchange resins were used for the isolation and purification of different products derived from plant or animal tissues, as it supports the reliable capture and removal of biologically active substances with a high molecular weight (Flengsrud et al, 2010;Huang et al, 2022;Pan et al, 2022). The different type of resins such as Dowex ® (Warda et al, 2003;Flengsrud et al, 2010), Amberlite (Vreeburg & Baauw, 2010), Lewatit (Linhardt et al, 1992), and Sephadex A-25 anion-exchange resin (Luppi et al, 2005) was designed and tested for heparin recovery.…”

Section: Introductionmentioning

confidence: 99%

The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

Milanović

Miladinović

2022

Food Sci. Technol

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The recovery of heparin from the porcine intestinal mucosa by using the ion exchange resin was studied. The procedure involved the following steps: alkaline hydrolysis, ion exchange resin adsorption, and elution. The activity of heparin was measured by an antifactor Xa assay. The ammonium chloride/ammonium hydroxide was used as a buffer, and the effect of salt concentration on the adsorption efficiency was evaluated. The results showed that the highest heparin yield (86.17%) was achieved at the lowest concentration of ammonium chloride. The pseudo-first-order model was shown as more adequate to describe heparin adsorption onto the resin.

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