Characterization of botanical origin of selected popular purple Eleutherococcus tea grown in Yunnan province of China and quantification of Its anthocyanins using spectrophotometric method

Abstract

Purple Eleutherococcus is a selected plant grown in high mountain in Puer region of Yunnan province, which is a rich source of anthocyanins with great potential as local vegetables and tea. However, the unknown botanical origin of purple Eleutherococcus restricted its edible value. Our objectives were to identify the botanical origin of purple Eleutherococcus by DNA marker combined with the morphological characteristics, optimaze the extration method of anthocyanins by orthogonal experiment method and determine the contents of anthocyanins in purple Eleutherococcus tea. The results revealed that the purple Eleutherococcus was more closely related to Eleutherococcus simonii through comparing the sequence alignment of ITS2. Combined with the morphological characteristics of plants, purple Eleutherococcus was finally identified and classified into Eleutherococcus simonii. Through orthogonal experiment, the optimal extraction conditions of anthocyanins from purple Eleutherococcus leaves were determined: the volume fraction of ethanol was 60%, the pH of ethanol solution was 3, and the water bath temperature was 60 °C. The ultrasonic extraction was conducted for 30 min. Our study provides scientific theoretical basis for the industrial extraction of anthocyanins and provides reference value for the further development of purple Eleutherococcus in Puer region of Yunnan province in China.

Hot-Air Flow Rolling Dry-Blanching Pretreatment Improves the Drying Quality of Acanthopanax sessiliflorus by Increasing the Drying Rate and Inactivating Enzymes

“…A. sessiliflorus fruits are usually dried by sun drying, hot air, and freeze drying, with dried products traditionally used as ingredients in wine or tea [ 6 ]. The skin of the fruits can prevent the migration of water during the drying process, resulting in a lower drying rate [ 7 ].…”

Inhibition of LPS-induced expression of iNOS and COX-2 on extracts of Acanthopanax leucorrhizus (Oliv.) Harms stems

“…Their roots and stem barks have traditionally been used as a tonic, as well as in the treatment of rheumatism and diabetes, chronic, bronchitis and so on (Li et al, 2016;Zou, et al, 2017;Chinese Pharmacopoeia Commission, 2015). Moreover, their leaves and roots are popularly used as a health drink and drug in China and Korea (Yook et al, 1999;Hu et al, 2012;Hu et al, 2018a;Nie et al, 2021;Chen et al, 2021). Acanthopanax leucorrhizus (Oliv.)…”