Stability and antioxidant activity of flavonoids from Lycium barbarum L. leaves during digestion in vivo

Abstract

It has been determined that Lycium barbarum L. leaves are a vital source of bioactive chemicals and have good potential for creating nutritious formulations. In this work, gavage injections of Lycium barbarum L. leaves flavonoids (LBLF) at various doses -50, 150, and 300 mg/kg BW -were administered to Sprague-Dawley (SD) rats. Four main LBLFs (rutin, chlorogenic acid, kaempferol, and quercetin) were assessed for stability and antioxidant activity in vivo. The findings demonstrated that within 4 h of the LBLF intervention, the plasma levels of rutin, chlorogenic acid, kaempferol, and quercetin were significantly dose-dependent. The rutin content peaked at 762.16 ± 36.63 μg/g at 1 h after the intervention, while the other three peaked at 383.07 ± 3.19 μg/g, 29.44 ± 1.95 μg/g and 18.511 ± 1.99 μg/g, at 3 h after the intervention respectively. Additionally, the levels of malondialdehyde (MDA) were significantly lower, and the activities of glutathione peroxidase (GSH-P x ), catalase (CAT), and total superoxide dismutase (T-SOD) were all considerably higher than those of the control group. This will establish a basis for elucidating the LBLF metabolic pathway in vivo, additional research into techniques to increase its stability, and investigation into its therapeutic activity.

Analyzing and comparising capacity of glucose- and uric acid-lowering effects of yellow tea with different varieties and grades in zebrafish model

“…Studies have consistently shown us that nutrition plays a big part in many diseases (Ji et al, 2022), and a healthy life style can help prevent many metabolic diseases, such as diabetes (Chen et al, 2023;Fan et al, 2022;Zapata et al, 2022). Furthermore, some chronic diseases require special diets that should be monitored by your physician.…”

Lycium barbarum (Wolfberry) Branches and Leaves Enhance the Growth Performance and Improve the Rumen Microbiota in Hu Sheep

“…It has been shown that Lycium barbarum leaves are rich in nutrients, including protein, minerals, nicotinic acid, polysaccharides, and flavonoids [ 15 , 16 ]. The leaves of Lycium barbarum are the main source of bioactive substances, with certain nutrients exceeding the contents found in the fruits [ 17 ]. Therefore, this suggests that the branches and leaves of Lycium barbarum (LBL) can serve as livestock forage.…”

Valorisation of goldenberry calyx: ultrasound-assisted extraction of phenolic compounds

“…It is based on the application of high-frequency sound waves between 20 and 100 kHz, generating a cavitation effect (Demirci et al, 2022;Xu et al, 2022). Several studies demonstrated its effectiveness as an emerging non-thermal technology during the extraction of phenolic compounds (Dadi et al, 2019;Dal Prá et al, 2015;Görgüç et al, 2022;Guandalini et al, 2019;Monteiro et al, 2022;Pradal et al, 2016), such as rutin, a flavanol characterised by its antioxidant capacity but whose bioavailability is low due to its low water solubility, poor chemical and metabolic stability, and limited membrane permeability (Fan et al, 2022;Zitha et al, 2022).…”