Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Abstract

A response surface method (RSM) was employed to optimize polysaccharide extraction from Panax japonicus (PJPS). Also, carboxymethyl substitution of PJPS (CM-PJPS) was studied using a response surface methodology. A three-variable Box-Behnken design (BBD) methodology was applied. RSM analyses revealed that conditions that maximized polysaccharide yield were as follows: a liquid-to-material ratio 22:1, an extraction temperature of 83.99 °C and an extraction duration of 2.32 h. Under optimum extraction conditions, the average absorbance value of PJPS was 0.6001, and the purity of PJPS was 98.2%. A quadratic regression model for CM-PJPS was obtained via BBD. The optimum extraction conditions for the process were determined to include a 1.38 h reaction time, 1.24 g monochloroacetic acid (MCA) and a reaction temperature of 52.85 °C. Under optimum extraction conditions, the average degree of CM-PJPS substitution was 0.9733. The structure of the PJPS was examined by means of Fourier transform infrared spectroscopy (FTIR). CM-PJPS exhibited the strongest anti-oxidant activity in vitro. In addition, CM-PJPS inhibited Skov3 and A2780 cancer cell proliferation. Our data revealed that CM-PJPS is a promising natural antioxidant with potential value as a food supplement and for treatment of cancer.

Characterization and antioxidant activities of polysaccharide extracted from Benincasa hispida var. chieh-qua How

“…However, no phytochemical research on Chieh qua was reported before. Natural polysaccharides have attracted more and more researchers' interest because of their various biological activities (Chen et al, 2021;He et al, 2020;Huang et al, 2015;Li et al, 2020Yu et al, 2021). Here we reported the antioxidant activities of water-soluble polysaccharide extracted from Chieh qua.…”

Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

“…The preparation method of CMLRP and the determination of the degree of substitution of CMLRP refer to published (Cheng et al, 2020;Yu et al, 2022).…”

“…The superoxide anion, ferrous ion, and hydroxyl radical scavenging activity were investigated as described by Yan and Yu (Yan et al, 2022;Yu et al, 2022). The appropriate solutions of LRP and CMLRP were prepared at concentrations of 1, 2, 4, 8, and 10 mg/mL for analysis of antioxidant activities.…”

Preparation and application of carboxymethylated and phosphatised Melaleuca polysaccharide

“…The most important biological activity of plant polysaccharides is their immunomodulatory effect of activating lymphocytes (Gan et al, 2021;Ji et al, 2019;Sun et al, 2021;Zhang et al, 2012). After molecular modification, various derivatives of polysaccharides with different structures and biological activities can be obtained Yu et al, 2022). The biological activity of polysaccharides can be improved by molecular modification.…”