Danzhi Jiangtang capsule regulates the metabolism of blood lipids in hyperlipidemic rats through JAK2/STAT3 pathway

Abstract

Danzhi Jiangtang capsule (DJC), a traditional medicinal formula composed of cortex moutan, heterophylly falsestarwort root, unprocessed rehmannia root, oriental waterplantain rhizome, dodder seed and leech, has been used to treat type 2 diabetes in clinic for almost 10 years. In addition, DJC was also confirmed that it could relieved oxidative stress of vascular endothelium in diabetic macroangiopathy. Based on the fact that the DJC could regulated the level of free fatty acids in diabetes patients and the constituents of DJC also possess potential anti-hyperlipidemic effect, thus, the present work aimed to evaluate the anti-hyperlipidemic effect of DJC and further investigated potential molecular mechanism of this action. As in vivo results revealed, DJC could reduced the levels of TC, TG, LDL, increased HDL level as well as decreased the level of ALT and AST of model rats. In addition, DJC also showed significant antioxidant effect by decreasing the level of MDA and increasing the level of SOD and GSH-Px. Furthermore, western blot analysis showed that DJC could up-regulated the expression of phosphorylated JAK2 and STAT3, and down-regulated the expression of SCOC3 protein, which indicated that DJC could regulate the metabolism of lipid via JAK2/STAT3 pathway.

Effect of lipoxin A4 methyl ester from arachidonic acid on JAK2/STAT3 pathway after cerebral ischemia-reperfusion injury

“…The inflammatory response is pivotal for cerebral ischemiareperfusion (I/R) injury (CIRI), where pro-and anti-inflammatory signals are activated within ischemic brains (Jin et al, 2013). Indeed, large amounts of inflammatory cytokines are released, including pro-and anti-inflammatory cytokines (Ye et al, 2010), and function primarily via the Janus kinase (JAK) /signal transducers and activators of transcription (STAT) pathway (Huang et al, 2022;Yin et al, 2022), which exists as potential transcription factors and induce alterations in the pattern of cell death or survival-related gene transcription (Jin et al, 2013;Xin et al, 2020). Family JAK comprises JAK1-3 and TYK2, while family STAT consists of STAT1-4, STAT5a, STAT5b, and STAT6 (Xin et al, 2020).…”