Identification of antioxidant ingredients by GC-MS from the essential oil of Purple Eleutherococcus simonii leaves

Abstract

The main objective of the current study was to investigate the components and antioxidant activity of essential oil extracted from Purple Eleutherococcus leaves (identified as Eleutherococcus simonii) (PEEO) by hydrodistillation (HD) and solid-phase microextraction (SPME). Fourteen chemical components were identified in PEEO using gas chromatography-mass spectrometry (GC-MS), accounting for 41.21% of the total essential oil extracted by SPME, including isopropyl toluene, 4-methylene-1-(1-methylethyl) cyclohexene (3.24%), fumaric-red myrrh alcohol (2.94%), farnesol acetate (2.92%), etc. Forty-three compounds were identified by HD, accounting for 73.28% of the total essential oil extracted by HD, including α-Farnesene (3.4%), Hexadecanoic acid, methyl ester (1.28%), (α-Bisabolol (0.87%), etc. The antioxidant activity of PEEO was characterized for 2,2-diphenyl-1picrylhydrazyl (DPPH) radical, ABTS method and FRAP method, and the IC 50 values were 1.125 mg/mL, 0.945 mg/mL and 0.862 mg/mL, respectively. The IC 50 values for DPPH, ABTS and FRAP of essential oil were similar to those of ascorbic acid (V C ; 0.853, 0.945 and 0.037 mg/mL). The results showed that the essential oil of PEEO has a high radical scavenging activity.

Using integrated GC-MS analysis, in vitro experiments, network pharmacology: exploring migao fatty oil active components/mechanisms against coronary heart disease

“…The antioxidant activity of MFO was measured in terms of radical scavenging ability, using a DPPH plant assay. All measurements were performed as follows: an amount of 1.0 mL of the five different concentrations of liquid extract was added to 2.0 mL of the DPPH• ethanol solution with a mass concentration of 0.1 mmol L -1 , and the absorbance readings were taken after 30 min against the blank at 517 nm (Zhang et al, 2022b;Nie et al, 2022). The antioxidant capacity was calculated using the following Equation 1where, (Ac: the absorbance of DPPH• solution and solvent, Ai: the absorbance of the sample solution and DPPH• solution, Aj: the absorbance of solvent solution and the sample): 2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging assay ABTS• + solution (7 mmol/L) and potassium persulfate solution (2.45 mmol/L) should be mixed in equal amounts.…”

Inhibition of LPS-induced expression of iNOS and COX-2 on extracts of Acanthopanax leucorrhizus (Oliv.) Harms stems

“…Their roots and stem barks have traditionally been used as a tonic, as well as in the treatment of rheumatism and diabetes, chronic, bronchitis and so on (Li et al, 2016;Zou, et al, 2017;Chinese Pharmacopoeia Commission, 2015). Moreover, their leaves and roots are popularly used as a health drink and drug in China and Korea (Yook et al, 1999;Hu et al, 2012;Hu et al, 2018a;Nie et al, 2021;Chen et al, 2021). Acanthopanax leucorrhizus (Oliv.)…”