Glutathione ameliorates Hypoxia/Reoxygenation (H/R) induced hepatocyte oxidative damage via regulating HO-1 signaling

Abstract

In our study, CCK-8 assay and LDH release detection were performed to detect the optimal protective concentration of GSH on HL7702 cell viability during H/R injury. HL7702 cells were randomly divided into four groups: Control group, H/R group, H/R+GSH group, and H/R+GSH+HO-1-siRNA group. Then, reactive oxygen species (ROS) was evaluated by DHE staning, MDA, T-SOD measurements; Cell injury was detected by CCK-8, LDH release, and supernatant AST and ALT levels; Apoptosis was determined by Hoechst staining and caspase 3 level. Compared with controls, H/R caused significant HL7702 cell injury evidenced as reduced cell viability, increased LDH release and apoptotic cell death (P < 0.01), with concomitant increases in ROS and MDA production (P < 0.01), while treated with GSH in H/R group significantly attenuated oxidative injury, enhanced cell viability and downregulated cell apoptosis (P < 0.01) together with HO-1 upregulation (P < 0.01). Knockdown HO-1 by its siRNA cancelled the protective effects of GSH from H/R compared with GSH group (P < 0.01). HO-1 was induced in HL7702 exposed to H/R injury and its level was obviously overexpressed after H/R injury with GSH treatment, suggesting its protective potential in GSH against H/R injury. GSH increases the expression of HO-1, which enhanced the early antioxidative activity and played a protective role against HL7702 cells H/R injury.

The recovery effect of Feixingcao tea (Teucrium viscidum Bl.) on inhibiting the decreased exercise ability caused by chronic alcoholic liver injury

“…When training in acute failure, hypoxia or hypoxia produces muscle damage and excessive energy consumption, particularly muscle fiber injury or pure mechanical strain, which destroys muscle tissue and causes enzymes in the muscle to seep into the blood, causing GPT and GOT activity to rise. GPT and GOT mainly exist in the liver, and liver damage can have a great impact on the regulation of GPT and GOT, thereby indirectly affecting exercise capacity (Chen et al, 2022). iNOS and TNF-α are involved in a variety of pathological processes in inflammation and immune response, and are important cytokines in inflammatory response.…”