Bioactive and Functional properties of gelatin peptide fractions obtained from sea bass (Dicentrarchus labrax) skin

Abstract

Fish skin is one of the most common resources of gelatin, which can be hydrolyzed bioactive peptides. In this study, gelatin from sea bass skin (SBS) was hydrolyzed with flavourzyme  to obtain peptide fractions with different molecular weights and determined their bioactive and functional properties. All peptide fractions obtained showed antioxidant activity (DPPH and FRAP). The bass gelatin peptide fraction 1 (BF1) (≤5 kDa) showed the highest DPPH (44.9%) and FRAP (42.04 mmol Fe +2 /g and 22.98 mmol trolox/g) activities. Besides, the BF1 (≤5 kDa) peptide fraction showed the highest in vitro cytotoxic effect (16.58%) at 20 mg/mL concentration compared to the other peptide fractions. The highest emulsifying capacity (389.5 m 2 /g), emulsifying stability (53.2 min), foaming capacity (30.47%), and foaming stability (10.40%) were obtained from the control gelatin sample. Moreover, the BF3 (≥10 kDa) peptide fraction showed an excellent fat binding capacity (9.39 mL/g). Enzymatic hydrolysis decreased emulsifying and foaming capacity of gelatin while increasing its fat binding capacity. In particular, antioxidant and anticancer activities of peptide fractions with low molecular weight were found to be high. The results demonstrated that gelatin and hydrolysates from the SBS offer an important alternative as a functional food ingredient for food technology.

Characteristics comparison of collagens from squid skin by different extraction methods

“…Amide I, II and III were related to the skeleton structure of peptide chain. The amide I bands of ASC, PSC and HSC caused by C=O stretching vibration along the peptide backbone were observed at about 1657, 1656 and 1656 cm -1 respectively, which was a sensitive indicator of secondary structure of the protein (Surewicz & Mantsch, 1988;Woo et al, 2008;Tekle et al, 2022). This confirmed that ASC, PSC and HSC had the typical triple helix structure of collagen, which was formed via the hydrogen bonds between N-H and C=O (Wang et al, 2018;Ahmed et al, 2018).…”

Recent advances in edible bird's nests and edible bird's nest hydrolysates

“…Bioactive peptides hydrolyzed from protein are the general term for 2-peptides to complex peptides composed of amino acids. Modern nutritional studies have pointed out that bioactive peptides hydrolyzed from protein can be more effectively absorbed by the body, thereby playing a specific role or improving the growth of intestinal flora to provide probiotic effects (Ji et al, 2022;Li et al, 2022;Tekle et al, 2022;Wei et al, 2022). For example, Ruann J. S. de Castro and Hélia H. Sato found that soy protein isolate hydrolysate had a 7-fold enhanced antioxidant activity compared with soy protein isolate (Castro & Sato, 2014).…”

Functional properties, structural characteristics and biological activities of deer blood hydrolysates obtained by using different protease

“…Amide I peak is the most intense absorption band in proteins, which is mainly associated with the C=O and C-N stretching vibrations and also relates to the backbone conformation (Tekle et al, 2022). Another prominent absorption band is the amide II peak, which is associated with in-plane N-H bending, and the C-N and C-C stretching vibrations (Van Wyk & Amonsou, 2022).…”