In vitro antioxidant and apoptotic activity of Lablab purpureus (L.) Sweet isolate and hydrolysates

Abstract

Cancer is a disease that invades the lives of millions of people each year. Chemotherapy is currently the most effective treatment however resulting in many adverse effects to the human body. Alternative treatments are being explored to overcome this obstacle. Peptides that possess radical scavenging activity and bioactive properties can be advantageous in prevention and treatment of chronic diseases. In this study, Lablab purpureus isolate and its hydrolysates (trypsin, pepsin and alcalase) were analysed for radical scavenging potential (DPPH, ABTS, superoxide radical scavenging and FRAP) and antiproliferative activity. Antiproliferative activity was confirmed with the peptides ability to induce apoptosis (Caspase 3/7 activity and Annexin V-PI). The lowest inhibitory concentrations (IC 50 ) for DPPH, ABTS and Superoxide radical scavenging ranged between 1.81-4.47, 1.73-2.42 and 1.36-4.41 mg/mL, respectively. FRAP ranged from 19.20 to 21.94 mg/mL. Generally, it is considered that a good antioxidant encompasses antiproliferative potential. Cell lines, A549, MCF-7 and HEK293, treated with pepsin hydrolysate showed (IC 50 values of 119.6, 9.80 and 13.86 µg/mL). The isolate and pepsin were chosen for apoptotic studies. The pepsin hydrolysate showed the highest inhibition in the cancerous cell lines (A549 and MCF-7) without greatly effecting normal cells (HEK293), and the isolate was selected for comparative analysis. Annexin V-PI staining showed cells in different stages of apoptosis (cells during early apoptosis; A549, 42%; MCF-7, 17%; HEK, 34%). Caspase 3/7 assay demonstrated that the peptide causes an increase in caspase activity. Peptides have the potential to act as chemo-preventative agents due to their antioxidant and apoptotic abilities.

Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway

“…Activated eNOS by Akt produces nitric oxide, leading to increased vascular permeability and cell migration (Yu et al, 2014). Caspase-3, -8 and -9 are all proteases involved in apoptosis (Sipahli et al, 2022). Caspase-8 and caspase-9 are upstream caspases in the apoptosis pathway and can activate downstream caspase-3 (Suo et al, 2016).…”

Lipidic compounds from the muscle of white shrimp (Litopenaeus vannamei): chemical structure and effect on the proliferation and morphology of human cancer cell lines

“…Protrusion of the plasmatic membrane, development of blebs, as well as microtubule peaks, were also possible to observe (Figure 2); these characteristics typically correspond to the later stage of apoptosis (Battistelli & Falcieri, 2020). It is pertinent to mention that one of the attributes sought in compounds with anticancer potential is that they cause cell death by apoptosis (Sipahli et al, 2022). However, further studies are considered necessary to determine the mechanism of death cell by which sub-fractions C5-3 and C5-4 might operate.…”

Preliminary Evaluation of Lablab purpureus Phytochemicals for Anti-BoHV-1 Activity Using In Vitro and In Silico Approaches

“…However, LP remains underutilized owing to its unpopularity, as compared to the other legumes, presumably due to its antinutritional factors and unappetizing nature. Nevertheless, the LP phytochemicals have now garnered scientific interest and are being researched concerning their potential biomedical properties including but not limited to antioxidant, anti-inflammatory, anticancer, antiviral, antifungal, and antibacterial effects. − In silico and in vitro investigations have reported dolichin, an antifungal chitinase-like compound, to be efficient in the inhibition of the human immunodeficiency virus-1 (HIV-1) by acting on the reverse transcriptase and glucosidase enzymes. , Luteolin has been observed to inhibit the in vitro viral entry and the action of neuraminidase enzyme in influenza (Influenza virus A/Fort Monmouth/1/1947 (H1N1)) . The lablab agglutinin known as Flt3 receptor-interacting lectin (FRIL), a mannose-binding macromolecule, was found to possess the ability to bind to the human immunodeficiency viral (HIV) glycoprotein and promote the formation of the viral syncytium, resulting in the reduction of the viral load .…”