Red ginseng polysaccharide alleviates cytotoxicity and promotes anti-inflammatory activity of ginsenosides

Kim, Hoon; Cho, Sung Min; Kim, Woo Jung; Hong, Ki‐Bae; Suh, Hyung Joo; Yu, Kwang-Won

doi:10.1590/fst.52220

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Sulfated galactans isolated from red alga showed no significant effect on cell viability, and a significant decrease in melanin levels in cells in a dose-dependent manner (Pratoomthai et al, 2018). Red ginseng polysaccharides prepared by ethanol fractionation also showed no cytotoxicity (Kim et al, 2022). Our findings confirmed that EP inhibited melanin synthesis activity in B16 cells, and that the lower the molecular weight (within a certain range), the better the tyrosinase inhibitory activity.…”

Section: Effects Of Epf5 On the Melanogenesis And Tyrosinase Activity...supporting

confidence: 70%

Preparation of low molecular weight Enteromorpha prolifera polysaccharide and its antioxidant and tyrosinase inhibitory activities

Que

Wang

et al. 2022

Food Sci. Technol

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Enteromorpha prolifera polysaccharide (EP) is a water-soluble sulfated polysaccharide with many types of biological activities. In this study, the crude polysaccharide was extracted from Enteromorpha prolifera and degraded by different physical, chemical, and biological methods. The results showed that the enzymatic degradation products displayed the best tyrosinase inhibitory activity. The optimal enzymatic degradation conditions were as follows: 30 °C, pH 5.5, 750 U/mL enzyme dosage for 2 h. The enzymatic hydrolysates were then divided into six EP fractions (EPFs) by an ultrafiltration membrane. The in vitro tyrosinase inhibitory activity of these fractions showed that the smaller the molecular weight of EPF, the better the inhibitory effect on tyrosinase. The antioxidant and tyrosinase inhibitory effect of the most effective EPF, a molecular weight below 5 kDa, (EPF5), was further confirmed on B16 cells. A kinetic study of tyrosinase inhibitory activity revealed that EPF5 is a competitive-uncompetitive mixed-type inhibitor of tyrosinase. These findings suggested that EPF5 has significant potential as a skin-whitening agent in cosmetics as well as an antibrowning agent in the food and agriculture industries.

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Section: Effects Of Epf5 On the Melanogenesis And Tyrosinase Activity...supporting

confidence: 70%

Preparation of low molecular weight Enteromorpha prolifera polysaccharide and its antioxidant and tyrosinase inhibitory activities

Que

Wang

et al. 2022

Food Sci. Technol

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“…A total of 50 mg polysaccharide was dissolved in 500 µl D 2 O. The 1 H NMR spectra, 13 C NMR spectra, 1 H-1 H COSY spectra, HMQC spectra and HMBC spectra were analyzed by the Varian Unity INOVA 400/45 (Varian Medical Systems, USA) and internal standard was tetramethylsilane (He et al, 2020;Kim et al, 2021).…”

Section: Nuclear Magnetic Resonance (Nmr) Assaymentioning

confidence: 99%

Comparative studies on the structure, biological activity and molecular mechanisms of polysaccharides from Boletus aereus (BA-T) and Pleurotus cornucopiae (PC-1)

2022

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In this study, two new polysaccharides were extracted from the fruiting bodies of Boletus aereus and Pleurotus cornucopiae, respectively. The results of structure identification showed that BA-T was composed of xylose residues, glucose residues and galactose residues, and the ratio is 3:6:6. The BA-T takes 1,6-glucose and 1,6-galactose as skeletons, extends a branched chain from galactose 2-O to connect 1,4-xylose, and connects β-4-glucose terminal monosaccharide to xylose. PC-1 was composed of of xylose, glucose and galactose in the ratio of 2:8:2 and takes 1,6-glucose as skeletons, extends a branched chain from galactose 4-O to connect (1→4,,6)-α-D-Glcp and (1→2,6)-α-D-galactose, and →4)-β-D-Glcp and →1)-α-D-Xylp as terminal group. The results of immunoactivity showed that BA-T and PC-1 have the proliferation activity of B cells, T cells and RAW264.7 cells in vitro, and the effect of BA-T on the proliferation of T cells was the greatest. The results of RNA-sequencing showed that the protein synthesis and metabolism of T cells are more vigorous under the stimulation of BA-T, and oxidative phosphorylation is the main energy source in the process of T cell proliferation.

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Carboxymethylated Desmodium styracifolium polysaccharide reduces the risk of calcium oxalate kidney stone formation by inhibiting crystal adhesion and promoting crystal endocytosis

Wang,

Liu,

Zhao

et al. 2024

Journal Cellular Physiology

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The inhibition of cell surface crystal adhesion and an appropriate increase in crystal endocytosis contribute to the inhibition of kidney stone formation. In this study, we investigated the effects of different degrees of carboxymethylation on these processes. An injury model was established by treating human renal proximal tubular epithelial (HK‐2) cells with 98.3 ± 8.1 nm calcium oxalate dihydrate (nanoCOD) crystals. The HK‐2 cells were protected with carboxy (‐COOH) Desmodium styracifolium polysaccharides at 1.17% (DSP0), 7.45% (CDSP1), 12.2% (CDSP2), and 17.7% (CDSP3). Changes in biochemical indexes and effects on nanoCOD adhesion and endocytosis were detected. The protection of HK‐2 cells from nanoCOD‐induced oxidative damage by carboxymethylated Desmodium styracifolium polysaccharides (CDSPs) is closely related to the protection of subcellular organelles, such as mitochondria. CDSPs can reduce crystal adhesion on the cell surface and maintain appropriate crystal endocytosis, thereby reducing the risk of kidney stone formation. CDSP2 with moderate ‐COOH content showed the strongest protective activity among the CDSPs.

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Red ginseng polysaccharide alleviates cytotoxicity and promotes anti-inflammatory activity of ginsenosides

Cited by 4 publications

References 25 publications

Preparation of low molecular weight Enteromorpha prolifera polysaccharide and its antioxidant and tyrosinase inhibitory activities

Preparation of low molecular weight Enteromorpha prolifera polysaccharide and its antioxidant and tyrosinase inhibitory activities

Comparative studies on the structure, biological activity and molecular mechanisms of polysaccharides from Boletus aereus (BA-T) and Pleurotus cornucopiae (PC-1)

Carboxymethylated Desmodium styracifolium polysaccharide reduces the risk of calcium oxalate kidney stone formation by inhibiting crystal adhesion and promoting crystal endocytosis

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