Polygonatum sibiricum polysaccharide inhibits IL-1β-induced inflammation in human chondrocytes

Wu, Jun; Zhang, Xinyu; Hu, Su-Qin; Pan, Suohua; Wang, Cunliang

doi:10.1590/fst.44021

Cited by 9 publications

(7 citation statements)

References 24 publications

(28 reference statements)

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“…Following that, cartilage degradation and bone erosion occur over time because of MMP’s and cytokine release (IL-1β and TNF-α). A role for macrophages and their linked inflammatory cytokines in knee OA has also been identified [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Carnosine Alleviates Knee Osteoarthritis and Promotes Synoviocyte Protection via Activating the Nrf2/HO-1 Signaling Pathway: An In-Vivo and In-Vitro Study

et al. 2022

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The most common joint disease in the elderly is knee osteoarthritis (OA). It is distinguished by cartilage degradation, subchondral bone loss, and a decrease in joint space. We studied the effects of carnosine (CA) on knee OA in male Wistar rats. OA is induced by anterior cruciate ligament transection combined with medial meniscectomy (ACLT+MMx) method and in vitro studies are conducted in fibroblast-like synoviocyte cells (FLS). The pain was assessed using weight-bearing and paw-withdrawal tests. CA supplementation significantly reduced pain. The enzyme-linked immunosorbent assay (ELISA) method was used to detect inflammatory proteins in the blood and intra-articular synovial fluid (IASF), and CA reduced the levels of inflammatory proteins. Histopathological studies were performed on knee-tissue samples using toluidine blue and hematoxylin and eosin (H and E) assays. CA treatment improved synovial protection and decreased cartilage degradation while decreasing zonal depth lesions. Furthermore, Western blotting studies revealed that the CA-treated group activated nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase (HO-1) and reduced the expression of cyclooxygenase-2 (COX-2). FLS cells were isolated from the knee joints and treated with IL-1β to stimulate the inflammatory response and increase reactive oxygen species (ROS). The matrix metalloproteinase protein (MMP’s) levels (MMP-3, and MMP-13) were determined using the reverse transcription-polymerase chain reaction (RT-PCR), and CA treatment reduced the MMP’s expression levels. When tested using the 2′,7′-dicholorodihydrofluroscene diacetate (DCFDA) assay and the 5,5′,6,6′-tetracholoro-1,1′,3,3′-tertraethylbenzimidazolcarboc janine iodide (JC-1) assay in augmented ROS FLS cells, CA reduced the ROS levels and improved the mitochondrial membrane permeability. This study’s investigation suggests that CA significantly alleviates knee OA both in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Carnosine Alleviates Knee Osteoarthritis and Promotes Synoviocyte Protection via Activating the Nrf2/HO-1 Signaling Pathway: An In-Vivo and In-Vitro Study

et al. 2022

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“…6. During KOA progression, under stimulated conditions, in ammatory cytokines activate NF-κB, which upregulates COX-2 and iNOS expressions, and induces expressions of other proin ammatory cytokines, namely, IL-6, TNF-α, NO, and PGE2 [41]. Moreover, excess expression of the aforementioned proin ammatory cytokines induce upregulation of metalloproteinase-3 (MMP-3) [42].…”

Section: Discussionmentioning

confidence: 99%

Yellow pond turtle peptides ameliorate inflammation and cartilage damage toxicity in papain-induced KOA rats by inhibiting COX-2 and iNOS expressions

Yan

Feng

et al. 2023

Preprint

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Objective The drugs in the treatment of knee osteoarthritis (KOA) have significant side effects and poor effectiveness. The plastron of yellow pond turtles is rich in small peptides. As such, it might inform potentially possible to uptake and enhance KOA drug utilization. Nevertheless, the cartilage protective activity of yellow pond turtle peptides (YPTP) in KOA and associated mechanism have not been reported. Method We explored KOA rat cartilage protection mediated by YPTP, and its underlying mechanisms using network pharmacology (NP), animal experimentation, and molecular docking (MD) analyses. Results Based on our NP data, COX-2 and iNOS were the primary target proteins of YPTP in KOA. Using an animal model, we revealed that YPTP strongly suppressed contents of COX-2, iNOS, and MMP-3 while upregulating COL II to alleviate inflammation and minimize cartilage damage. In addition, MD analysis further validated the 5 YPTP-derived peptides that interacted with COX-2 and iNOS via hydrogen bonding and hydrophobic interactions. Of note, the reduced YPTP dose employed in this investigation enhanced IL-1beta contents in synovial membranes, which requires further exploration. Conclusion YPTP ameliorated inflammation and cartilage damage in papain-induced KOA rats by suppressing both COX-2 and iNOS expressions.

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“…The I κ B α is an important mechanism for the activation and repression of NF- κ B [ 35 ]. We found that GLA decreased the levels of p-p65 and suppressed the p65 accumulation in nucleus, which indicated that GLA prevented NF- κ B pathway activation [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Glaucocalyxin A Attenuates IL-1β-Induced Inflammatory Response and Cartilage Degradation in Osteoarthritis Chondrocytes via Inhibiting the Activation of NF-κB Signaling Pathway

Zhu

Zhang

et al. 2022

Disease Markers

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Glaucocalyxin A (GLA) is a bioactive natural compound with anti-inflammatory activity. Herein, the role of GLA in osteoarthritis (OA) was evaluated. Our results demonstrated that the IL-1β-induced inducible nitric oxide synthase (iNOS) and cyclooygenase-2 (COX-2) expression, two enzymes resulting in the release of nitric oxide (NO) and PGE2, were also prevented by GLA in chondrocytes. Moreover, GLA suppressed inflammatory cytokines production in chondrocytes. In addition, the elevated expressions of MMPs and ADAMTSs and the degradation of aggrecan and collagen II were reversed by GLA in chondrocytes. Furthermore, GLA decreased p-p65 level and suppressed the nuclear p65 accumulation in the nucleus of chondrocytes. Collectively, we concluded that GLA attenuated inflammatory response in chondrocytes via NF-κB pathway. These findings suggested that GLA might become an effective agent for OA treatment.

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Polygonatum sibiricum polysaccharide inhibits IL-1β-induced inflammation in human chondrocytes

Cited by 9 publications

References 24 publications

Carnosine Alleviates Knee Osteoarthritis and Promotes Synoviocyte Protection via Activating the Nrf2/HO-1 Signaling Pathway: An In-Vivo and In-Vitro Study

Carnosine Alleviates Knee Osteoarthritis and Promotes Synoviocyte Protection via Activating the Nrf2/HO-1 Signaling Pathway: An In-Vivo and In-Vitro Study

Yellow pond turtle peptides ameliorate inflammation and cartilage damage toxicity in papain-induced KOA rats by inhibiting COX-2 and iNOS expressions

Glaucocalyxin A Attenuates IL-1β-Induced Inflammatory Response and Cartilage Degradation in Osteoarthritis Chondrocytes via Inhibiting the Activation of NF-κB Signaling Pathway

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