MicroR-146 protects against rat ischemia-reperfusion injury by targeting NF-κB-mediated PI3K/AKT/mTOR signaling pathway

Wang, Leyuan; Liu, Guofang; Shao, Zetao; Zhang, Qianqian; Yin, Lili; Xu, Enbo; Li, Biao; Cui, Xiangxing; Teng, Hongtao

doi:10.1590/fst.36820

Cited by 5 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, the research on methane efficacy after TBI is not very extensive, it's essential to find out the probable mechanisms of its protective effect. Potts et al, 2006 andWang et al, 2021a suggested to regard inflammation, oxidative injury, and ironmediated damage as potential therapeutic targets of brain injury. Based on previously reported biological characteristics of methane (Song et al, 2015;Wang et al, 2017b;Yao et al, 2017), we supposed that methane protects brain via anti-inflammation, anti-oxidant and anti-apoptotic mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury

Wang

et al. 2022

Food Sci. Technol

View full text Add to dashboard Cite

Clinically, methane is an anti-inflammatory gas used to treat organ damage. There is no extensive research on methane's repair of traumatic brain injury (TBI), so this study aims to investigate the role of methane and its potential mechanism. A rat TBI model was established by mechanical impact. SD rats were divided into four groups, including sham group, TBI group, 2 or 20 mL/kg methane-rich saline (MS) group. Morris water maze test revealed that MS improved cognitive function in TBI rats. HE and Nissl staining showed that MS reduced the blurring of brain tissues boundary, the number of necrotic plaques and neurons in TBI rats. In addition, ELISA revealed that MS restored the oxidative stress of TBI rats and reversed the inflammatory effect of TBI on brain cells, and TUNEL staining detected that MS inhibited cell apoptosis. Western blotting showed that MS downregulated Caspase-3 and Bax protein levels and upregulated Bcl-2 level. And MS inhibited phosphorylated expressions of Janus kinase 1 (JAK1), signal transducer and activator of the transcription (STAT1) and NF-κb-p65. In conclusion, MS improved cognitive function, reduced inflammatory response and oxidative stress and inhibited cell apoptosis to relieve rat TBI by blocking the activation of the JAK1/STAT1/NF-κb-p65 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury

Wang

et al. 2022

Food Sci. Technol

View full text Add to dashboard Cite

show abstract

“…The PI3K-Akt pathway has intrinsic effects on the proliferation of cancer cells (Wang et al, 2022), inhibits PI3Kδ signaling through BCR and other pathways, promotes FL cell death, and restores FL cell dependence on Bcl-2 anti-apoptotic protein (McCubrey et al, 2006). Bcl-2 family proteins are apoptosis-regulating proteins and play a key role in apoptosis (Volkmann et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway

TAO

et al. 2022

Food Sci. Technol

View full text Add to dashboard Cite

The purpose of this study was to observe its in vitro inhibitory effect on human thyroid cancer cell line SW579 through PI3K-Akt pathway. In this study, the survival of cells and the inhibitory effect of gardenia tea extract (GTE) on SW579 cancer cells were firstly observed by MTT method. Further qPCR experiments were used to observe mRNA expression in SW579 cancer cells. The active compound composition in the GTE was then also determined by HPLC. The experimental results showed that GTE had almost no effect on normal primary thyroid cells and had no toxic effect, but GTE could significantly inhibit the proliferation of SW579 cancer cells. The detection of mRNA expression in SW579 cancer cells showed that GTE could upregulate the expressions of Bax, caspase-3, caspase-8 and caspase-9 and down-regulate the expressions of Bcl-2, Bcl-xL, PI3K and Akt in SW579 cancer cells. The chemical composition test showed that GTE mainly contains three compounds, namely rutin, quercetin and phloretin. It can be seen that gardenia tea can regulate the PI3K-Akt pathway of SW579 cancer cells through the effective active substances contained in it, and exert its anti-cancer effect. It is a health-care tea with good anti-cancer effect.

show abstract

“…LPS is an endotoxin inducing inflammation and is used to create neurodegenerative in vitro models (Oh et al, 2021). LPS induces microglial cells activate inflammation through AKT/STAT, NF-κB, and MAPKs signaling pathways, these cells generate too many inflammatory mediators for instance NO and PGE 2 , at the same time pro-inflammatory cytokines TNF-α, IL-6, and IL-1 (Wang et al, 2021a;Wang et al, 2021b;Jung et al, 2009;Ryu et al, 2019;Zhao et al, 2019). Thus, it is significant to regulate MAPK, NF-κB, and the AKT/STAT pathways for treating neuroinflammatory diseases and controlling microglial activation can be used to treat apoptosis and inflammation in neurodegenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Zingiber officinale attenuates neuroinflammation in LPS-stimulated mouse microglia by AKT/STAT3, MAPK, and NF-κB signaling

et al. 2022

View full text Add to dashboard Cite

To explore the protective activity of ginger (Zingiber officinale) root ethanol extract (GRE) on the neuroinflammation induced by lipopolysaccharide in microglial cells. Ginger has been investigated as a neuroprotective and anti-aging agent. Nevertheless, ginger extract attenuates neuroinflammation in microglia have not been discovered in depth. The results showed that GRE had high total phenolic and (55.63 ± 0.16 mg GAE/g) and total flavonoid content (4.33 ± 0.17 mg QUE/g), and antioxidant activity. GRE inhibited the release of cytokines and inflammatory mediators including COX-2, PGE 2 , Nitric oxide, interleukin-6, TNF-α, and iNOS. GRE ameliorated microglia-mediated neuronal insults via upregulating the expression of Bax and reducing the expression of Bcl-2. GRE suppressed NF-κB and AKT/STAT3, and the MAPK pathway in the neuroinflammatory response. In conclusions, GRE positively affected anti-neuroinflammatory and neuroprotective activity without serious side effects, which might be used as a functional food additive and/or therapeutic material for the management and prevention of neurodegenerative diseases.

show abstract

MicroR-146 protects against rat ischemia-reperfusion injury by targeting NF-κB-mediated PI3K/AKT/mTOR signaling pathway

Cited by 5 publications

References 29 publications

Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury

Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury

Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway

Zingiber officinale attenuates neuroinflammation in LPS-stimulated mouse microglia by AKT/STAT3, MAPK, and NF-κB signaling

Contact Info

Product

Resources

About