Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images

Lucas, Bruna Nichelle; NORA, DALLA; Boeira, Caroline Pagnossim; Verruck, Silvani; Rosa, Cláudia Severo da

doi:10.1590/fst.35122

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…Folin-Ciocalteu assay TPC was measured by using the Folin-Ciocalteu colorimetric method, according to the standard methodology (23). The standard was gallic acid, and the absorbance was read at 760 nm.…”

Section: Polyphenolic Content and Antioxidant Activity Assaysmentioning

confidence: 99%

Antioxidant activity and phenolic acid constituents of two andean Hypericum L. species from Colombia

Andrea

Rojano²,

Arbeláez³

et al. 2022

Plant Sci. Today

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Antioxidant capacity of extracts of different polarity obtained from two Hypericum L. species (H. juniperinum and H. mexicanum) was assessed by means of total polyphenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, ferric reducing antioxidant power (FRAP) assay and oxygen radical absorbance capacity (ORAC) assay. Their phenolic acid composition was also determined by HPLC. The ethyl acetate extract of H. juniperinum was the most active in the ABTS, FRAP and TPC assays with 10867.48 ?mol TEAC/g, 242.80 mg AAE/g and 491.08 mg GAE/g respectively. On the other hand, the methanol extract obtained from H. mexicanum appeared as the most active extract in the DPPH assay (3714.23 ?mol TEAC/g). Similarly, the butanol fraction coming from the methanolic extract of H. mexicanum showed the highest activity in the ORAC assay (12910.06 ?mol TEAC/g). HPLC analysis of the extracts revealed the presence of phenolic acid compounds, such as chlorogenic (50.09 mg/g) and p-coumaric acids (63.36 mg/g) in H. mexicanum and p-coumaric acid (8.45 mg/g) in H. juniperinum. A high correlation between antioxidant activity and total polyphenol content was established. Specifically, H. mexicanum exhibited the highest ORAC capacity, which may be associated with the high content of chlorogenic and p-coumaric acids present in medium to polar extracts. Our results constitute a significant contribution to the study of antioxidant activity and the determination of the phenolic acid profile in both species. The analysed extracts showed promising antioxidant activity that could be useful in the pharmaceutical, cosmetic and food industries.

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Section: Polyphenolic Content and Antioxidant Activity Assaysmentioning

confidence: 99%

Antioxidant activity and phenolic acid constituents of two andean Hypericum L. species from Colombia

Andrea

Rojano²,

Arbeláez³

et al. 2022

Plant Sci. Today

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“…The total phenolic content of TR and AtPAP1 root extracts was determined using the Folin–Ciocalteau colorimetric assay [ 46 ] with slight modifications using gallic acid as a standard to prepare a calibration curve. Briefly, 0.5 mL of extract (400 µg/mL) solution was mixed with 2.5 mL of 10% Folin–Ciocalteu reagent and 2 mL of 7.5% of sodium carbonate solution.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation and In Silico Calculations of the Antioxidant and Anti-Inflammatory Properties of Secondary Metabolites from Leonurus sibiricus L. Root Extracts

Merecz-Sadowska,

Sitarek,

Kowalczyk

et al. 2023

Molecules

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Leonurus sibiricus L. has great ethnobotanical and ethnomedicinal significance. This study aimed to assess the antioxidant and anti-inflammatory properties of Leonurus sibiricus L. transgenic roots extracts transformed by Rhizobium rhizogenes, with and without the AtPAP1 transcriptional factor. The study determined the total phenolic and flavonoid contents, as well as in vitro antioxidant assays, including hydrogen peroxide and nitric oxide scavenging activity. In addition, in silico computational studies and molecular docking were conducted to evaluate the antioxidant and anti-inflammatory potential of the identified compounds. The ligands were docked to NADPH oxidase, cyclooxygenase 2,5-lipoxygenase, inducible nitric synthase and xanthine oxidase: enzymes involved in the inflammatory process. The total phenolic and flavonoid contents ranged from 85.3 ± 0.35 to 57.4 ± 0.15 mg/g GAE/g and 25.6 ± 0.42 to 18.2 ± 0.44 mg/g QUE/g in hairy root extracts with and without AtPAP1, respectively. H2O2 scavenging activity (IC50) was found to be 29.3 µg/mL (with AtPAP1) and 37.5 µg/mL (without AtPAP1 transcriptional factor), and NO scavenging activity (IC50) was 48.0 µg/mL (with AtPAP1) and 68.8 µg/mL (without AtPAP1 transcriptional factor). Leonurus sibiricus L. transformed root extracts, both with and without AtPAP1, are a source of phytochemicals belonging to different classes of molecules, such as flavonoids (catechin and rutin), phenolic compounds (caffeic acid, coumaric acid, chlorogenic acid, ferulic acid) and phenylpropanoid (verbascoside). Among the radicals formed after H removal from the different -OH positions, the lowest bond dissociation enthalpy was observed for rutin (4′-OH). Rutin was found to bind with cyclooxygenase 2, inducible nitric synthases and xanthine oxidase, whereas chlorogenic acid demonstrated optimal binding with 5-lipoxygenase. Therefore, it appears that the Leonurus sibiricus L. transformed root extract, both with and without the AtPAP1 transcriptional factor, may serve as a potential source of active components with antioxidant and anti-inflammatory potential; however, the extract containing AtPAP1 demonstrates superior activities. These properties could be beneficial for human health.

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“…Gallic acid was used as standard and for the calculation of the percentage of inhibition the following relation was used: %Inhibition = [Blank − Test]/Blank × 100. A second test to evaluate the antioxidant activity was carried out through the determination of total phenols by the ABTS and Folin–Ciocalteu technique, according to the methodology previously reported [ 28 , 29 , 30 , 31 ]. The same doses mentioned above were used for the test, and the results were reported as equivalents of gallic acid.…”

Section: Methodsmentioning

confidence: 99%

Cymbopogon citratus Essential Oil: Extraction, GC–MS, Phytochemical Analysis, Antioxidant Activity, and In Silico Molecular Docking for Protein Targets Related to CNS

Cortes-Torres,

López-Castillo,

Marín-Torres

et al. 2023

CIMB

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This study analyzed the chemical composition of Cymbopogon citratus essential oil from Puebla, México, assessed its antioxidant activity, and evaluated in silico protein–compound interactions related to central nervous system (CNS) physiology. GC–MS analysis identified myrcene (8.76%), Z-geranial (27.58%), and E-geranial (38.62%) as the main components, with 45 other compounds present, which depends on the region and growing conditions. DPPH and Folin–Ciocalteu assays using the leaves extract show a promising antioxidant effect (EC50 = 48.5 µL EO/mL), reducing reactive oxygen species. The bioinformatic tool SwissTargetPrediction (STP) shows 10 proteins as potential targets associated with CNS physiology. Moreover, protein–protein interaction diagrams suggest that muscarinic and dopamine receptors are related to each other through a third party. Molecular docking reveals that Z-geranial has higher binding energy than M1 commercial blocker and blocks M2, but not M4 muscarinic acetylcholine receptors, whereas β-pinene and myrcene block M1, M2, and M4 receptors. These actions may positively affect cardiovascular activity, memory, Alzheimer’s disease, and schizophrenia. This study highlights the significance of understanding natural product interactions with physiological systems to uncover potential therapeutic agents and advanced knowledge on their benefits for human health.

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Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images

Cited by 10 publications

References 18 publications

Antioxidant activity and phenolic acid constituents of two andean Hypericum L. species from Colombia

Antioxidant activity and phenolic acid constituents of two andean Hypericum L. species from Colombia

In Vitro Evaluation and In Silico Calculations of the Antioxidant and Anti-Inflammatory Properties of Secondary Metabolites from Leonurus sibiricus L. Root Extracts

Cymbopogon citratus Essential Oil: Extraction, GC–MS, Phytochemical Analysis, Antioxidant Activity, and In Silico Molecular Docking for Protein Targets Related to CNS

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