Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

Abstract

On this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an OC 50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%, indicating the prepared immunoaffinity column is suitable for use as an efficient extraction method to detect DAN residues in food products.

Design, Synthesis, and Dual Evaluation of Quinoline and Quinolinium Iodide Salt Derivatives as Potential Anticancer and Antibacterial Agents

“…[34] Quinoline is a specific inhibitor of bacteria type II topoisomerase, namely DNA gyrase. [35][36][37][38][39][40] The compounds have potential anticancer and antitumor effects owing to the structural similarity between the two gyrase enzymes.…”

“…The structural uniqueness of quinoline is not only used widely in energy, agriculture, organic luminescent materials, etc., but also plays an important role in medicine, including antibacterial, anticancer, antitumor, and anti‐HIV . Quinoline is a specific inhibitor of bacteria type II topoisomerase, namely DNA gyrase . The compounds have potential anticancer and antitumor effects owing to the structural similarity between the two gyrase enzymes.…”