Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS

Medina, Bianca Gonçalves; Sartori, André Victor; Moraes, Maria Heloísa Paulino de; Cardoso, Maria Helena Wohlers Morelli; Jacob, Silvana do Couto

doi:10.1590/fst.33717

Cited by 14 publications

(10 citation statements)

References 47 publications

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“…Fumonisin B1 (FB1) with 100% purity (HPLC/ESI-MS) was obtained from Fumizol Ltd. (Szeged, Hungary). The FB1 solution was prepared in acetonitrile/water (1:1, v/v) (Medina et al 2019) and subsequently, diluted with water to obtain 1 mM FB1 final concentration. Thereafter, tomato plants were exposed to 1 µM and 10 µM concentrations of FB1 in the nutrient solution.…”

Section: Fumonisin B1 Treatmentsmentioning

confidence: 99%

Fumonisin B1-Induced Oxidative Burst Perturbed Photosynthetic Activity and Affected Antioxidant Enzymatic Response in Tomato Plants in Ethylene-Dependent Manner

Iqbal

Czékus

Angeli³

et al. 2022

J Plant Growth Regul

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Fumonisin B1 (FB1) is a harmful mycotoxin produced by Fusarium species, which results in oxidative stress leading to cell death in plants. FB1 perturbs the metabolism of sphingolipids and causes growth and yield reduction. This study was conducted to assess the role of ethylene in the production and metabolism of reactive oxygen species in the leaves of wild type (WT) and ethylene receptor mutant Never ripe (Nr) tomato and to elucidate the FB1-induced phytotoxic effects on the photosynthetic activity and antioxidant mechanisms triggered by FB1 stress. FB1 exposure resulted in significant ethylene emission in a concentration-dependent manner in both genotypes. Moreover, FB1 significantly affected the photosynthetic parameters of PSII and PSI and activated photoprotective mechanisms, such as non-photochemical quenching in both genotypes, especially under 10 µM FB1 concentration. Further, the net photosynthetic rate and stomatal conductance were significantly reduced in both genotypes in a FB1 dose-dependent manner. Interestingly, lipid peroxidation and loss of cell viability were also more pronounced in WT as compared to Nr leaves indicating the role of ethylene in cell death induction in the leaves. Thus, FB1-induced oxidative stress affected the working efficiency of PSI and PSII in both tomato genotypes. However, ethylene-dependent antioxidant enzymatic defense mechanisms were activated by FB1 and showed significantly elevated levels of superoxide dismutase (18.6%), ascorbate peroxidase (129.1%), and glutathione S-transferase activities (66.62%) in Nr mutants as compared to WT tomato plants confirming the role of ethylene in the regulation of cell death and defense mechanisms under the mycotoxin exposure.

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Section: Fumonisin B1 Treatmentsmentioning

confidence: 99%

Fumonisin B1-Induced Oxidative Burst Perturbed Photosynthetic Activity and Affected Antioxidant Enzymatic Response in Tomato Plants in Ethylene-Dependent Manner

Iqbal

Czékus

Angeli³

et al. 2022

J Plant Growth Regul

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“…The MMIPs can be separated from the solvent by external magnetic field due to the magnetic properties of magnetic nanoparticles [16]. It is worth reporting that their low toxicity combined with the skills above make MMIPs a qualified option in the fields of purification and separation [17]. Magnetic solid phase extraction, based on the MMIPs, combined with high-performance liquid chromatography (MSPE-HPLC) at optimal conditions was successfully used for the extraction of STG.…”

Section: Magnetic Solid Phase Extraction (Mspe)mentioning

confidence: 99%

Magnetic Nanomaterials and Nanostructures in Sample Preparation Prior to Liquid Chromatography

Antoniou

Samanidou

2022

Magnetochemistry

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Magnetic nanomaterials and nanostructures compose an innovative subject in sample preparation. Most of them are designed according to the properties of the target analytes on each occasion. The unique characteristics of nanomaterials enhance the proficiency at extracting and enriching due to their selective adsorption ability as well as easy separation and surface modification. Their remarkable properties, such as superparamagnetism, biocompatibility and selectivity have established magnetic materials as very reliable options in sample preparation approaches. In order to comprehend the range of utilization at magnetic materials and nanostructures, this review aims to present the most notable examples in sample preparation prior to liquid chromatography (LC) to the community of analytical chemists. Primarily, the review describes the principles of the techniques in which the magnetic materials are utilized and leaned on. Additionally, there is a diligent report about the novel magnetic techniques and finally a comparison to demonstrate the total point of view.

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“…Water-acetonitrile acidified with acetic or formic acids (~15-33/85-66/1-2% v/v) provides over 60% recovery of a wide spectrum of mycotoxins (Da Silva et al, 2019;Juan et al, 2016;Medina et al, 2019;Romera et al, 2018;Sulyok et al, 2007). QuEChERS is a good alternative for DAS, especially for the analysis of matrixes reach in coextractives and in case analyte enrichment is essential for proper method performance (Perestrelo et al, 2019).…”

Section: Detection Of Mycotoxins In the Dry Samplementioning

confidence: 99%

Mycotoxins in herbal tea: transfer into the infusion

Chalyy

Kiseleva

Седова

et al. 2021

WMJ

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Mycotoxins are natural food contaminants, associated with adverse health effects due to acute intoxication and, what is much more common, chronic exposure. The most hazardous and widespread mycotoxins are subjected to regulation in food in most countries. Alongside with regulated, a wide list of mycotoxins is monitored in various foods. Traditionally mycotoxins in tea are determined in a dry sample, not taking into account their transfer rate into the infusion. This study was aimed at the determination of the transfer rate of several mycotoxins from naturally contaminated herbal tea samples into an infusion. Seven of the most contaminated samples were pre-selected during the monitoring of mycotoxins in Camellia sinensis and herbal tea available in the Russian Federation. Ochratoxin A (OTA), sterigmatocystin (STE), mycophenolic acid (MPA), tentoxin (TTX), alternariol (AOH), its methyl ether (AME), zearalenone (ZEN), enniatins A and B (ENN A and B), beauvericin (BEA) were detected in these samples in the range of several μg/kg to several mg/kg. HPLC-MS/MS was used for dry tea samples and their infusion analysis. Mycotoxin polarity and infusion pH (for analytes possessing carboxylic groups) appeared to be factors determining transfer rate. STE transferred into infusion at the average rate of 10%. Average transfer of Alternaria toxins varied from 73% (TTX) to 45% (AOH) and about 11% (AME). A third part of ZEN was detected in the infusion. Transfer of ENNs and BEA was low and did not exceed 7%. Infusion pH affected MPA transfer rate; it increased from 23% to 96% in the pH range from 5.5 to 6.3. 83% of OTA was detected in the infusion of a single contaminated sample. Consideration of the mycotoxin transfer rate to herbal tea infusions resulted in the change of the model herbal tea input into mean chronic dietary exposure for most studied mycotoxins.

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Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS

Cited by 14 publications

References 47 publications

Fumonisin B1-Induced Oxidative Burst Perturbed Photosynthetic Activity and Affected Antioxidant Enzymatic Response in Tomato Plants in Ethylene-Dependent Manner

Fumonisin B1-Induced Oxidative Burst Perturbed Photosynthetic Activity and Affected Antioxidant Enzymatic Response in Tomato Plants in Ethylene-Dependent Manner

Magnetic Nanomaterials and Nanostructures in Sample Preparation Prior to Liquid Chromatography

Mycotoxins in herbal tea: transfer into the infusion

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