Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction

Caldeira, Ana Clara Rocha; Franca, Wanderley Filho Lopes de; Converti, Attílio; Lima, William James Nogueira; Sampaio, Fábio Coelho; Faria, Janaína Teles de

doi:10.1590/fst.32218

Cited by 15 publications

(9 citation statements)

References 36 publications

(50 reference statements)

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“…All systems showed partition coefficients (k) > 1, which indicates that anthocyanins migrated preferentially to the alcohol-rich top phase. This result, which may be ascribed to the salting-out effect of salt on anthocyanin molecules, agrees with the partitioning preference of anthocyanins for the alcohol phase observed in ethanol-based ATPS [9,11,13,44]. Moreover, it should not be forgotten that the phenolic structure of anthocyanins is responsible for their hydrophobic nature, which makes them soluble in most of the organic solvents [39].…”

Section: Partitioning Of Anthocyaninssupporting

confidence: 87%

“…The anthocyanins‐rich crude extract was obtained from mature black plum ( Syzygium cumini ) fruits according to Caldeira et al. 9. After washing and rinsing the fruits, peel (∼ 140 g) was manually recovered and added to 280 mL of HCl solution (pH 2.0) in a 500‐mL glass flask protected from light, which was then placed in an orbital shaking incubator (Lucadema, São Paulo, Brazil) under 200 rpm stirring for 24 h at 298 K. The extract was filtered through qualitative paper (0.8 µm) and concentrated by lyophilization (Alpha 2‐4, Martin Christ, Osterode am Harz, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Aqueous two-phase systems (ATPS) have been widely used in separations of biomolecules such as proteins, polypeptides, enzymes [1][2][3][4][5][6], bioactive compounds [7,8], and natural dyes [9][10][11][12][13][14][15], among others. Since both phases are mainly made up of water, they provide mild favorable conditions needed to preserve activities of biomolecules [16].…”

Section: Introductionmentioning

confidence: 99%

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Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

et al. 2022

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Partitioning of anthocyanins contained in the crude extract of Syzygium cumini fruit was investigated in aqueous two‐phase systems (ATPS) made up of isopropanol and Na2SO4/(NH4)2SO4. Na2SO4 induced ATPS formation more effectively than (NH4)2SO4. The increase in temperature enhanced phase separation in the (NH4)2SO4 ATPS, while the opposite occurred in the Na2SO4 ATPS. The higher the overall mixture concentration or temperature, the higher the values of tie‐line length and slope. In all systems and conditions, anthocyanins preferentially partitioned to the top phase. The partition coefficient and theoretical recovery yield in the top phase varied in the ranges 1.14–1.77 and 53.31–63.87 % in the (NH4)2SO4 ATPS, and in the ranges 1.94–21.50 and 65.90–95.55 % in the Na2SO4 ATPS.

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Section: Partitioning Of Anthocyaninssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

et al. 2022

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“…10 Aqueous two-phase system (ATPS) is a liquid-liquid system, and consists of two hydrophilic components, such as two polymers, polymer and salt, alcohol and salt, which spontaneously form two immiscible phases in a static state under certain conditions. 12,13 In recent years, aqueous two-phase extraction (ATPE) has been successfully used to extract polysaccharides due to the merits of simple procedure, the superior extraction efficiency, the environmental-friendly and low cost. 10,13 In particular, ATPS of ethanol/salt aqueous has attracted a lot of attention in extracting polysaccharides from plants owing to low viscosity, easy demixing and solvent recycling.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasound‐assisted aqueous two‐phase extraction of polysaccharides from seabuckthorn fruits using response methodology, physicochemical characterization and bioactivities

et al. 2022

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Background: Seabuckthorn fruits contains many active subtances, among them, the seabuckthorn polysaccharide is one of the main active ingredients, and exhibits diverse bioactivities. The extraction of polysaccharides from seabuckthorn fruits is the most important step for their wide applications. Ultrasound-assisted aqueous two-phase extraction (UA-ATPE) is a promising green method for extracting polysaccharides. Additionally, physicochemical characterization and antioxidant activities can evaluate the potential functions and applications in the food and medicine industries.Results: Based on the single-factor experiments, 20.70% (w/w) ammonium sulfate ((NH 4 ) 2 SO 4 ) and 27.56% (w/w) ethanol were determined as the suitable composition for aqueous two-phase. The optimum conditions of UA-ATPE obtained by response surface methodology were as follows: ultrasonic power (390 W), extraction time (41 min), liquid-to-material ratio (72: 1 mL/g), and the total yield of the polysaccharides reached 34.14 ± 0.10%, The molecular weights of the purified upper-phase seabuckthorn polysaccharide (PUSP) and the purified lower-phase seabuckthorn polysaccharide (PLSP) were 65 525 and 26 776 Da, respectively. PUSP and PLSP contained the same six monosaccharides (galacturonic acid, rhamnose, xylose, mannose, glucose and galactose), but with different molar ratios. Furthermore, PUSP and PLSP displayed certain viscoelastic property, had no triple helical structure, possessed different thermal stability, surface morphology and conformation in aqueous solution. PUSP and PLSP displayed strong antioxidant properties by the assays of scavenging ability of ABTS + •, the protection of DNA damage and erythrocyte hemolysis.Conclusion: UA-ATPE significantly increased the yield of seabuckthorn polysaccharides. PUSP and PLSP were different in many aspects, such as molar ratio, surface shape and antioxidant activities. Seabuckthornpolysaccharides possess great potential in medicine and functional foods.

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“…In some fields, ATPS is a novel alternative technique to conventional solvent extraction. ATPS has successfully been used for the extraction and purification of peptides [9], polysaccharides [10], enzymes [11], heavy metals [12], proteins [13], amino acids [14], cells [15], and cytochromes [16], and has also been used for the separation and enrichment of neutral, anionic, and cationic ions [17]. In recent years, some novel, low-cost, and efficient ATPSs have appeared that are different from typical polymer/salt and ionic liquid/salt systems.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk

Jiang

Zhong

et al. 2021

Separations

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Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components in raw milk and the pre-treatment of raw milk is complex. In this study, a new pretreatment method combined with ion chromatography (IC) for rapid and sensitive determination of thiocyanate is proposed. An acetonitrile/(NH4)2SO4 aqueous two-phase system (ATPS) was developed for the separation and enrichment of thiocyanate in raw milk. Response surface methodology was performed to optimize the extraction conditions and an efficient pretreatment were obtained using ATPS composed of 42% acetonitrile (w/w) and 16% (NH4)2SO4 (w/w), with the pH 4.7, and the recovery of thiocyanate reached 107.24 ± 0.5%, and the enrichment ratio was 10.74 ± 0.03. IC was used to establish a thiocyanate enrichment method. The linear range was from 0.05 to 15 mg/L and R2 = 0.998, the limit of detection (LOD) was 0.2 μg/L, the limit of quantification (LQD) was 0.6 μg/L. Hence, it is feasible to combine ATPS with IC for the enrichment and determination of thiocyanate in raw milk.

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Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction

Cited by 15 publications

References 36 publications

Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

Optimization of ultrasound‐assisted aqueous two‐phase extraction of polysaccharides from seabuckthorn fruits using response methodology, physicochemical characterization and bioactivities

Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk

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