A new polysaccharide isolated from Dendrobium offcinale, stimulates aquaporin-3 expression in human keratinocytes

Abstract

Polysaccharides are the major components of the water extract from Dendrobium officinale, which have been thoroughly studied and found to have favorable moisturizing properties on mammalian skin. However, the structures of polysaccharides on skin moisturizing properties remains unknown. The aim of this study was to extract and characterize D. officinale polysaccharides, which have anti-oxidant activity and the role of promoting effects of aquaporin-3 (AQP3). Two crude polysaccharide fractions were extracted using water and biological fermentation, respectively. The crude polysaccharides were further purified by graded alcohol precipitation, and finally purified polysaccharides DOP-S and DOP-F were obtained by Sephacryl S-300 column chromatography. The average molecular weights of DOP-S and DOP-F were 3.604 × 10 5 and 1.027 × 10 5 Da, respectively. Monosaccharide units and structures of DOP-S and DOP-F were further elucidated by gas chromatography-mass spectrometry (GC-MS) and Fourier Transform infrared spectroscopy (FTIR). At the same time, DOP-S and DOP-F induced aquaporin-3 (AQP3) expression was evaluated by immuno-fluorescence analysis. The antioxidant activities of the polysaccharides were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacities, and ferric reducing antioxidant power (FRAP). The results showed that biological fermentation effectively achieved the hydrolysis of the polysaccharides and stimulated antioxidant activity and AQP3 expression.

Efficacy of Dendrobium candidum polysaccharide extract as a moisturizer

“…Several research groups recently reported that polysaccharide extracts derived from D . officinale exhibit good moisturizing effects 15,29 . In 2021, Li et al found that polysaccharides isolated from D .…”

“…15,29 In 2021, Li et al found that polysaccharides isolated from D. officinale enhanced the expression of aquaporin-3 in human keratinocytes. 15 Although the extracts exhibit a few moisturizing effects, the underlying mechanism in the human skin was unknown.…”

“…Several studies demonstrated that D . candidum inhibited bacteria, promoted epithelial cell proliferation, enhanced immunity against aging, and resisted oxidative damage 13–15 . Recently, a study reported that the ultrafine powder of D .…”

Characterization and antioxidant activities of polysaccharide extracted from Benincasa hispida var. chieh-qua How

“…The aqueous extracts were combined and concentrated under reduced pressure and then precipitated with 75% ethanol at 4 ºC overnight. The precipitate was centrifuged and re-dissolved in distilled water to remove the protein using Sevage method (CHCl 3 : n-BuOH, 4: 1, v/v) (Li et al, 2021b). Finally, the samples were lyophilized, giving the polysaccharide (BHCP).…”

Long-term results of laparoscopic surgery and open surgery for colorectal cancer in Huaihe River Basin of China

“…Postoperative patients are prone to multiple complications and the prognosis is not satisfactory (Kim & Kim, 2017;Chen et al, 2018b;Bellio et al, 2019). Therefore, actively exploring alternative treatments for colorectal cancer has become the focus of clinical research (Li et al, 2021;Mostafa et al, 2021;Jouki et al, 2020). In recent years, with the continuous development of minimally invasive techniques, laparoscopic surgery has been widely used in clinical treatment because of its small surgical trauma, low amount of bleeding, and rapid recovery of postoperative patients (Hamabe et al, 2019;Tajima et al, 2017;Hamabe et al, 2019;Iwamoto et al, 2019).…”