Isolation, characterisation and continuous culture of Lactobacillus spp. and its potential use for lactic acid production from whey

Abstract

Bioprospecting of microorganisms with the potential of exploiting agro-industrial wastes is an active field of biotechnological research. In this study, we aimed to isolate a microorganism adapted to the dairy matrix with specific characteristics to produce lactic acid from dairy industry wastes, such as whey. Whey was collected from the agricultural region of Antioquia, Colombia, and samples were cultured on Man-Rogosa-Sharpe modified medium using lactose as a source of carbon. The most promising strain isolated was characterised, and its adaptation to whey was determined. A gram-positive Lactobacillus was isolated and named as G1. This strain grows in deproteinised whey with low protein requirements, has a homofermentative metabolism and exhibits high tolerance to low pH (≤5.0). The G1 strain is a promising bacterium for use in lactic acid production from whey, which is an underutilised by-product of the dairy industry.

Immobilization of Lactic acid bacteria for production of extracellular polysaccharides

“…LAB are generally recognized as safe microorganisms and produce EPS with a wide diversity of structures with no health risk (Surayot et al, 2014;Thummar & Ramani, 2016;Ryan et al, 2015). EPS produced by LAB have recently received increasing attention because of their health benefits to the consumers (Ishiguro et al, 2017;Mejia-Gomez & Balcázar, 2020). EPS produced by LAB possess various potential health benefits and have important functional roles in human or animal health including immunomodulatory properties, anti-cancer, antioxidant activity, anti-ulcer (Abid et al, 2018;Deepak et al, 2016;Zhang et al, 2015), anti-biofilm agents to prevent adhesion of pathogenic bacteria, blood glucose (Oleksy & Klewicka, 2018;Kanak & Yilmaz, 2021;Pato et al, 2021) and cholesterol lowering properties (Korcz et al, 2018), and antihypertensive activity (Harutoshi, 2013).…”