miRNA-1180 suppresses HCC cell activities via TRAF1/NF-κB signaling pathway

Abstract

The miRNA-1180 anti-cancer effects in hepatocellular carcinoma cell were studied. 33 hepatocellular carcinoma patients were collected. In cancer and adjacent normal tissue from patients, TRAF1 protein and miR-1180 expression was determined by HE staining, IHC and RT-PCR methods. In the cell experiments, HepG2 cells were divided into three groups: NC group, BL group and miRNA group. The cells of NC group were untreated ; BL group were transfected with empty camer; miRNA group were transfixed with miRNA-1180. We determinates cell proliferation rate of difference groups, measuring the cell apoptosis rate and cell cycle of difference groups by flow cytometry, detected cell invasion and migration abilities of difference groups by transwell and wound healing assay and evaluating relative proteins expressions by WB assay. Compared with Normal liver tissue, cell infiltrations were significantly increased. miR-1180 was negative correlation with TRAF. The cell proliferation rate of miRNA group was significantly lower than that of NC group; The cell apoptosis and G1 phase rates were significantly difference among three groups; however, P53 and P21 protein expressions were significantly increased in miRNA groups. Over-expression miRNA-1180 had effect to inhibit HepG2 cell proliferation, invasion, migration and improve HepG2 cell apoptosis by regulation TRAF1/NF-κB signaling pathway.

Evaluate the effect of licorice on anti-liver fibrosis: a systematic review and meta-analysis

“…Abdel-Wahab et al (2021) and Chen et al (2017) investigated the effect of licorice roots extract on some metabolic pathways and their regulating miRNAs in hepatocellular carcinoma cells, the result various beneficial effects of licorice roots extract including induction of apoptosis and cell cycle arrest, upregulating tumor suppressor miRNAs; let7a-3p, miR-34c-5p, miR-122-5p, miR-126-3p, miR195-5p, miR-199a-5p, miR-206, and miR326-5p and inhibiting HIF1a, PI3K and C-Myc and activating AMPK and p53. Notably, mirnas have been shown to be involved in the development of fibrosis (Abdel-Wahab et al, 2021;Zheng & Wang, 2020). Fourth, inhibiting enzymes of glycolysis; HK-2, LDH-A and PK-M2.…”

Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway

“…Phosphorylated Bad protein can bind to 14-3-3 protein, thus preventing Bad protein from binding to Bcl-2 protein on mitochondrial membrane. Therefore, the inhibition of apoptosis by Bad protein phosphorylation is essential (Huang, 2020;Zheng & Wang, 2020). Previous studies have shown that the PI3K/AKT pathway inhibits mitochondrial-mediated apoptosis in papillary thyroid cancer (PTC) cells by promoting phosphorylation of Bad (Dieci et al, 2018;Esmailpoor et al, 2019).…”

Zingiber officinale attenuates neuroinflammation in LPS-stimulated mouse microglia by AKT/STAT3, MAPK, and NF-κB signaling

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