An exploratory to analysis the effects of the dirrerent roles of mathca on lipid metabolism and intestinal flora regulation between normal and diabetic mice fed a high-fat diet

Abstract

Fast food is becoming increasing popular as a social phenomenon, and it usually contains high fat contents. Matcha is one versatile tea, and its application in food brings lots of new consumers. Herein, a high-fat diet containing matcha was prepared, and in this study we investigated the effects of such a diet on lipid metabolism and intestinal flora of normal and diabetic mice. Results showed that diabetes had significant weight loss, hyperphagia, hyperlipidemia and intestinal flora disturbance, with particularly significantly increased Alistipes, Prevotella, Helicobacter, Acetatifactor and Bacteroides, and decreased Alloprevotella, Lactobacillus, Allobaculum and Akkermansia. In diabetes, matcha decreased serum triglyceride and LDL-C, increased HDL-C, reversed those bacteria trends besides Alistipes, Prevotella and Akkermansia. In normal mice, matcha decreased serum LDL-C, increased Parabacteroides, Bacteroidales_unclassified, Erysipelotrichaceae_unclassified and Barnesiella, Lachnospiraceae_unclassified, and decreased Helicobacter and Clostridium XlVa. Most importantly, matcha increased Porphyromonadaceae_unclassified, Lactobacillus, Alloprevotella, Prevotella and Allobaculum; and decreased Bacteroides and Enterobacteriaceae_unclassified in diabetes, however these changed bacteria in normal mice showed an opposite trend from diabetes. Intestinal flora balance is vital important to host, matcha helps to improve the balance of lipid metabolism and intestinal flora according to different character of host, and is a valuable addition to develop functional food.

Studying and enhancing the hypoglycemic effect of Pingyang Yellow Soup and preliminary exploration of its internal mechanisms in a zebrafish model

“…As can be seen from Table 5 and Figure 8, within the range of observed actual dose concentration, the action curves of TPs and its main catechin monomer in PYS solution mainly showed a downward opening parabolic shape (quadratic). The results showed that, under the cross-influence of multiple factors, in microecological balance (Liu et al, 2022a;Zhao and Zhang, 2020); and affect and change the internal and external transport efficiency of nutrients, including downregulating the expression of dependent glucose cotransporter 1 (Hossain et al, 2002), protecting islet cells and promoting insulin secretion (Wang et al, 2010), alleviating insulin signal blockade, and promoting sugar oxidation (Bose et al, 2008) and lipid metabolism in vivo (Huang et al, 2013).…”

“…Diabetes mellitus is a worldwide disease; the number of patients has increased significantly in recent years, and it has become one of the major diseases threatening human health (Li et al, 2020). The results based on studies of modern model animals (Liu et al, 2022a;Liu et al, 2022c;Zhang et al, 2020) and people (Adu et al, 2022;Mortazavi et al, 2018) showed that tea has great potential in promoting the balance of blood glucose.…”

Restoration and preservation effects of mung bean antioxidant peptides on H2O2‐induced WRL‐68 cells via Keap1‐Nrf2 pathway

“…A high‐fat diet can cause elevated levels of free radicals and lipid metabolism disorders in the body. However, mung bean peptides have been reported to improve high‐fat‐induced disorders and significantly increase levels of liver antioxidant enzymes and indicators, such as total cholesterol and triglycerides (Liu, 2022 ).…”

“…Kusumah et al ( 2020 ) researched the antioxidant potential of a mung bean proteolytic solution by measuring its 2,2′‐Azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonate) radical scavenging, ferrous ion chelating ability, and oxygen radical absorption capacity. Xie et al ( 2019 ) and Liu ( 2022 ) proved that mung bean protein hydrolysate has a good protective effect on hydrogen peroxide (H 2 O 2 )‐induced NCTC‐1469 cells, and it has a significant promotional effect on hepatic HDL and antioxidant enzyme levels in high‐fat‐induced mice. These results implied that the antioxidant capacity of mung bean protein hydrolysate is associated with the regulation of body metabolism.…”