Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

Abstract

In this study, we extracted lotus root polysaccharide (LRP) and synthesized carboxymethylated lotus root polysaccharide (CM-LRP) using response surface methodology (RSM). The monosaccharide component of LRP was analyzed by pre-column derivatization high performance liquid chromatography (PCD-HPLC). The polysaccharide was characterized by ultraviolet (UV) spectroscopy scan, Fourier transform infrared spectroscopy (FTIR), circular dichroism spectrometer (CD), and scanning electron microscopy (SEM). It was found that the main monosaccharide component of LRP was glucose, accounting for more than 98% of the total polysaccharide component. RSM analyses revealed that the optimal conditions for CM-LRP synthesis were a reaction duration of 2.22 h, the chloroacetic acid dosage is 2.02 g, and a temperature of 46.87 °C. Under these conditions, the predicted degree of substitution (DS) was determined to be 0.5101. The superoxide anion, ferrous ion and hydroxyl radical antioxidant system were constructed to study the antioxidant activity of LRP and CMLRP. The activity of CMLRP to remove ferrous ions and hydroxyl radicals were significantly stronger than that of LRP, and it also showed a certain concentrationdependent. LRP has a better scavenging ability than CMLRP in scavenging superoxide anion free radicals. Our data revealed that CM-LRP is a promising natural antioxidant with potential value as a food supplement.

Bioactive polysaccharides from lotus as potent food supplements: a review of their preparation, structures, biological features and application prospects

“…LPs also exhibited significant reducing power and anti-lipid peroxidation activity ( 27 , 56 , 69 ). Moreover, the root LPs effectively prevented the oxidative damage of red blood cells induced by H 2 O 2 ( 15 ), which is consistent with Yan et al ( 86 ). A plumula LPs (PNP) effectively prevented oxidative damage in RAW264.7 cells by reducing the production of lactate dehydrogenase (LDH), reactive oxygen species (ROS), and malondialdehyde (MDA), improving the activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), and stimulating the production of GSH ( 66 ).…”

“…Appropriate chemical modifications have been shown to enhance the efficacy of LPs and broaden their potential applications ( 39 ). Recent studies showed that phosphorylation and carboxymethylation could significantly improve the scavenging ability of LRPs on ABTS, ·OH radicals, and ferrous ions ( 69 , 89 ). This enhancement is likely attributed to the introduction of carboxymethyl and phosphate groups, which alter the water solubility and spatial conformation of LRPs, thereby influencing their biological activity ( 110 ).…”

Effect of sodium trimetaphosphate modification on the structure and rheological properties of zein

“…The FT-IR spectra of zein and Z-SP were obtained on a Nico-let FT-IR 20 SX spectrometer (Nicolet, USA) based on the KBr method (Yan et al, 2022;Forato et al, 2003). The measurements were carried at 4 cm -1 resolution with 32 scans in the mid-infrared region (4000-400 cm -1 ).…”

Optimization of extraction technology of alkaloids in lotus leaf based on BP neural network

“…Studies have shown that the lotus leaf alkaloid is insoluble in water, although it can be absorbed and involved in metabolism under the condition of lipid solubility in the body, but the onset of action is slow and cannot be fully utilized. Another study found that the alkaloid salts have good water solubility, the alkaloid in the lotus leaf are extracted in the form of salt, which is not only conducive to optimizing the resources, improving the utilization rate and the economic benefits, but also facilitates better absorption by the body (Yan et al, 2022b). Therefore, it has important academic value and application prospect to study the optimization of extraction process conditions of lotus leaf alkaloids.…”