Fermented black rice bran extract inhibit colon cancer proliferation in WiDr cell lines

Safrida, Safrida; Budijanto, Slamet; Nuraida, Lilis; Priosoeryanto, Bambang Pontjo; SAEPULOH, Uus; MARYA, Sela Septima; Ardiansyah, Ardiansyah; Shirakawa, Hitoshi

doi:10.1590/fst.14422

Cited by 4 publications

(2 citation statements)

References 41 publications

(51 reference statements)

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“…Previous studies also showed that EBRB is not toxic to HUVEC cells [10]. Moreover, a study showed that EBRB did not have the toxicity effect in Vero cells with an IC50 value of 1139.5 μg/mL [56]. Cytotoxicity is classified into four categories based on IC50 values.…”

Section: Cytotoxicity Assay Ebrb 'Sembada Hitam' On Vero Cellsmentioning

confidence: 99%

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Aprilianti,

Purwestri,

Saragih

et al. 2024

J.Trop.Life.Science

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Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2-induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.

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Section: Cytotoxicity Assay Ebrb 'Sembada Hitam' On Vero Cellsmentioning

confidence: 99%

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Aprilianti,

Purwestri,

Saragih

et al. 2024

J.Trop.Life.Science

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show abstract

“…In recent years, with the in-depth study of the composition and structure of wheat bran, it has been found that wheat bran is rich in phenols which are beneficial to human health (Lee et al, 2020;Xu et al, 2018). Pathological studies show that these phenols have important functional properties and pharmacological effects, and have potential application value in the prevention of many degenerative diseases, such as obesity, cancer, diabetes, cardio-cerebrovascular diseases and so on (Safrida et al, 2022;Zhao et al, 2017). Phenols in wheat bran mainly exist in two forms: soluble free phenol and insolublebound phenol.…”

Section: Introductionmentioning

confidence: 99%

Penicillium fermentation combined with enzyme treatment to enhance the release of phenolic acids from wheat bran

CHAI

ZHONG

et al. 2023

Food Sci. Technol

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Phenols from wheat bran play an important role in improving the physiological function of human body because of their potential antioxidant capacity. However, most of the phenols in wheat bran exist in the form of insoluble binding, which limits their application in the field of food and medicine. In this paper, the modification of wheat bran by Penicillium solid-state fermentation (SSF) was studied. It was found that the content of free phenolic acids in wheat bran was significantly correlated with the activity of key hydrolytic enzymes in the fermentation process. On the fifth day of Penicillium fermentation, the content of free phenolic acids was the highest, which was 750.98 μg/g, which was 1.78 times higher than that of the control. The release of free phenolic acids was highest when wheat bran was treated with compound enzyme at 300 U/mL. Furthermore, the structure of wheat bran was significantly changed by fermentation with enzyme addition. On the 5th day of fermentation, the content of free phenolic acids was as high as 1500 μg/g, which was twice as much as that of fermented bran and 3.56 times that of unfermented bran.

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Dietary supplementation of black rice bran to colon carcinogen-induced mice: Examining the development of colorectal cancer by improving environmental colon conditions

Budijanto¹,

Nurtiana²,

Muniroh³

et al. 2023

Heliyon

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Fermented black rice bran extract inhibit colon cancer proliferation in WiDr cell lines

Cited by 4 publications

References 41 publications

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Penicillium fermentation combined with enzyme treatment to enhance the release of phenolic acids from wheat bran

Dietary supplementation of black rice bran to colon carcinogen-induced mice: Examining the development of colorectal cancer by improving environmental colon conditions

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