Biological activities of Persea americana: in vitro and in vivo studies

Al-Otaibi, Tahani; Hawsah, Maysar Abu; Alojayri, Ghada; Al-Shaebi, Esam M.; Dkhil, Mohamed A.; Thagfan, Felwa A.; El‐Khadragy, Manal F.; Al‐Quraishy, Saleh; Abdel‐Gaber, Rewaida

doi:10.1590/fst.123722

Cited by 5 publications

(1 citation statement)

References 57 publications

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“…The majority of anticoccidial drugs, including sulfaquin‐oxaline, nitrofurazone, nicarbazin, amprolium, prevent coccidian from growing, metabolizing, and reproducing normally. Drug resistance and residues have, however, started to appear as a result of the extensive usage of these drugs (Al‐Otaibi et al, 2023). Plants and their constituent parts have shown promise as potential alternatives to chemical anticoccidial drugs since they have less side effects (Wunderlich et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

In vitro studies for antiparasitic activities of Punica granatum extract

Al‐Shaebi,

Al‐Quraishy,

Maodaa

et al. 2023

Microscopy Res & Technique

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Because of the drug resistance, medicinal plants are used more frequently than coccidiostats to treat and control coccidiosis. Punica granatum is a powerful antioxidant with a variety of medicinal uses. This study used an in vitro experiment to investigate how different P. granatum from Yemen (Y) and Egypt (E) sources affected oocyst sporulation and served as an anthelminthic effector. In contrast to PGE and mebendazole, PGY (200 mg/mL) has the shortest time to paralyze and death the earthworm Eisenia fetida in this investigation. In addition, the treated worm groups' cuticle thickness and shrinkage in comparison to the control group were assessed and contrasted. Eimeria papillata is used as a model protozoan parasite in anticoccidial assays. This study shows that P. granatum affects oocysts sporulation in a dose‐dependent manner, with maximal percentages of 100% (PGY) and 48.60% (PGE) at 96 h for P. granatum concentrations of 200 mg/mL. Inhibition (%) was compared to various detergents, as well as positive and negative controls. According to our research, the P. granatum extract had powerful anthelmintic and anticoccidial properties, with the potency changing according to the environmental conditions of each fruit source.Research Highlights Habitat of the plant is useful for production and accumulation of some secondary metabolites in plants which be effective for the therapeutic uses. Different parameters in the environmental ecosystem affecting variation in chemical compositions and biological activity of P. granatum.

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Section: Introductionmentioning

confidence: 99%

In vitro studies for antiparasitic activities of Punica granatum extract

Al‐Shaebi,

Al‐Quraishy,

Maodaa

et al. 2023

Microscopy Res & Technique

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Anticoccidial and jejunum‐protective effects of Krameria lappacea roots extract on experimental Eimeria papillata infection

Alamari,

Abdel‐Gaber,

Al‐Shaebi

et al. 2024

Microscopy Res & Technique

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Coccidiosis is a protozoan parasitic disease caused by Eimeria species and affects wild and domestic animals. Coccidiostats are currently available to control this disease, although drug resistance has been confirmed for all of them. As a result, there is an urgent need to identify eco‐friendly agents to control and treat this disease. This study aimed to investigate the ameliorative role of the Krameria lappacea roots extract (KLRE) on the outcome of coccidiosis induced by Eimeria papillata. Male C57BL/6 mice were divided into seven groups (5 mice/group), as follows: Group 1: noninfected‐nontreated (control group), Group 2: noninfected‐treated group with KLRE (200 mg/kg), Group 3: infected‐nontreated group, Group 4: infected‐treated group with KLRE (50 mg/kg), Group 5: infected‐treated group with KLRE (100 mg/kg), Group 6: infected‐treated group with KLRE (200 mg/kg), and Group 7: infected‐treated group with amprolium (120 mg/kg). Groups (3–7) were inoculated orally with 1 × 103 sporulated E. papillata oocysts. One hour after infection, groups (4–6) were daily treated for 5 days with KLRE and amprolium. On day 5 postinfection, oocyst output was determined, and mice were euthanized for the collection of jejuna then preparation of histological sections and jejunal homogenate was used for the determination of biochemical and oxidative damage markers. The coccidial infection induced weight loss of mice by 3.971%, which improved after KLRE to −1.512%. After KLRE treatment, the rate of feed intake was improved to be 52.21 ± 2.30 than those in infected group (40.47 ± 2.25). Oocyst output was significantly reduced in mice treated with KLRE (1.308 × 106 oocysts/g.feces) compared with those in the infected group (5.387 × 106 oocysts/g.feces). E. papillata infection induced marked histological alterations within jejunum tissue. After treatment, KLRE was able to impair the development of parasite stages (meronts, gamonts, and developing oocysts) in the jejunum through a significant reduction of number and size in comparison with the infected group. Infection with E. papillata induced a disturbance in the nutrient absorption in the jejunal mice tissue, which improved after the treatment with KLRE and amprolium. Also, KLRE counteracted significantly the E. papillata‐induced loss of reduced glutathione and total antioxidant capacity. Our findings indicate that KLRE could be used as an alternative to the available coccidiostats currently available.Research Highlights Krameria lappacea exhibit significant anticoccidial and antioxidant activities induced by E. papillata infection. Krameria lappacea exhibit significant improvement in the pathological alterations of the jejunal tissue induced by E. papillata infection.

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In vitro antiparasitic activity of ethanolic leaves extract of Anethum graveolens

Maodaa,

Al-Quraishy,

Abdel-Gaber

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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Natural products are safe environmentally friendly agents and have no negative impact on the environment, they can be used to combat parasitic diseases. Helminthiasis and coccidiosis are parasitic diseases that harm both health and the economy. This research aimed to see how Anethum graveolens leaves extract (AGLE) worked as an anti-parasitic modulator during oocyst sporulation of an Eimeria papillata infection. FT-IR phytochemical analysis revealed the presence of eight compounds. The time required to induce paralysis and death in worms at the highest concentration (200 mg/mL) was 4.57±0.26 and 5.22±0.10 min, respectively. In an in vitro study, AGLE (300 mg/ml) inhibited sporulation by approximately 100% after 72 and 96 hr. AGLE (200, 100, and 50 mg/ml), amprolium, DettolTM, and phenol induced variable inhibition levels at 96 hr of 5.54%, 1.01%, 37.33%, 81.33%, and 89.33%, respectively. Our findings suggest that AGLE has potent anthelmintic and anticoccidial properties that could be further developed into a novel therapeutic agent.

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Biological activities of Persea americana: in vitro and in vivo studies

Cited by 5 publications

References 57 publications

In vitro studies for antiparasitic activities of Punica granatum extract

In vitro studies for antiparasitic activities of Punica granatum extract

Anticoccidial and jejunum‐protective effects of Krameria lappacea roots extract on experimental Eimeria papillata infection

In vitro antiparasitic activity of ethanolic leaves extract of Anethum graveolens

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