Isolation, characterization, and antimicrobial evaluation of bacteriocin produced by lactic acid bacteria against Erwinia carotovora

Pato, Usman; Riftyan, Emma; Jonnaidi, Nia Naidya; Wahyuni, Mimi Sri; Feruni, Jeska Aryaningsih; Abdel-Wahhab, Mosaad A.

doi:10.1590/fst.11922

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…E. carotovora is a Gram-negative bacterium which affects most vegetables and fruits in the field and during shipment or in storage [ 58 ]. E. carotovora infect the host plant through some injuries and degrade its cell wall, followed by tissue maceration leading to the soft rotting of stems and fruits [ 9 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…Erwinia carotovora is a Gram-negative bacterium with rod-shaped cells which decay most of the vegetables and fruits in the field and during shipment or its storage. These edibles are contaminated by the E. carotovora after rainfall and irrigation [ 9 ]. E. carotovora is one of the plant pathogens known to cause blackleg and soft rot diseases in potato crop [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

et al. 2023

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In recent years, the biological synthesis of silver nanoparticles has captured researchers’ attention due to their unique chemical, physical and biological properties. In this study, we report an efficient, nonhazardous, and eco-friendly method for the production of antibacterial silver/silver chloride nanoparticles utilizing the leaf extract of Stachys emodi. The synthesis of se-Ag/AgClNPs was confirmed using UV-visible spectroscopy, DPPH free radical scavenging activity, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). An intense peak absorbance was observed at 437 nm from the UV-visible analysis. The Stachys emodi extract showed the highest DPPH scavenging activity (89.4%). FTIR analysis detected various bands that indicated the presence of important functional groups. The SEM morphological study revealed spherical-shaped nanoparticles having a size ranging from 20 to 70 nm. The XRD pattern showed the formation of a spherical crystal of NPs. The antibacterial activity performed against Erwinia carotovora showed the maximum inhibition by centrifuged silver nanoparticles alone (se-Ag/AgClNPs) and in combination with leaf extract (se-Ag/AgClNPs + LE) and leaf extract (LE) of 98%, 93%, and 62% respectively. These findings suggested that biosynthesized NPs can be used to control plant pathogens effectively.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

et al. 2023

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“…Notably, the composition of the medium did not affect the cellular development of the enterocins-producing microorganisms. In addition the production of bacteriocins depends on the growth conditions including medium composition, pH, temperature, water activity, and others (Pato et al, 2022a). Fact, to cell growth, peptide-rich substrates may act as inducers or precursors of bacteriocin biosynthesis (Parlindungan;Dekiwadia;Jones, 2021;Vázquez et al, 2005;Yang et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Soybean flour as a substrate to obtain Enterococcus durans bacteriocins

Bússolo

Souza

et al. 2022

Ciênc. agrotec.

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Natural preservatives, such as enterocins, have been the focus of several studies for use in the food industry. However, the commercial media used to obtain enterocins are still expensive, presenting a disadvantage for large-scale production. In this study was developed four formulations of culture media containing soybean flour for obtaining Enterococcus durans enterocins. The antilisterial activity of E. durans MF5 enterocins obtained in soybean and MRS media (control) was characterized, with Listeria monocytogenes CLIP2032 and L. innocua CLIP12612 as the bacterial strains. The growth of E. durans (CFU/mL) was significantly affected by the incubation time in the soybean and MRS media (p <0.05), but the composition of the media did not affect the cell development of the enterocin-producing microorganism. When evaluating the genes encoding enterocin synthesis, positive results were obtained for enterocin A, B, P, and X. When treated with proteolytic enzymes (α-chymotrypsin, protease, and proteinase-K), enterocin was inactivated, confirming its protein character. The antilisterial activity of the enterocins obtained in soybean media was up to 6,400 AU mL-1. Enterocins from soybean media M1 and M2 showed antibacterial activity at a concentration of 1 mg/mL after 6 h incubation. Thus, we show that culture media formulated with soybean flour are promising substrates for enterocin production that would allow the protocol to be expanded on a large scale.

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“…Further studies by inhibiting the mechanism of bacterial-host cell interactions will help provide new ideas for drug targeting actions. In recent years, more and more studies have confirmed that edible probiotics and their metabolites are widely used for food preservation and regulation of human health and immunity (Pato et al, 2022;Shi et al, 2022). Therefore, in the food industry, it is more highlighted that the selection and breeding of high-yielding PLA strains, metabolic regulation, and the mechanism of their inhibition of spoilage and pathogenic bacteria still need more in-depth research, so that the application of PLA is more extensive.…”

Section: Discussionmentioning

confidence: 99%

A new anti-colon cancer tumor pathway of Phenyllactic acid by reducing adhesion of Fusobacterium nucleatum

WANG

et al. 2023

Food Sci. Technol

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The incidence of colorectal cancer (CRC) is increasing, with 1,931,590 new colorectal cancer patients worldwide in 2020. Fusobacterium nucleatum (Fn) has been widely reported as a harmful microorganism that promotes the development of CRC. Fn was found to enhance proliferation and metastasis of CRC cells by regulating cellular glucose metabolism and amino acid metabolism in trials. In a previous study, phenyl lactic acid (PLA), a natural metabolite of various probiotic bacteria, was found to have the ability to inhibit Fn as an organic acid. Therefore, further in-depth analysis of the mechanism of action of PLA in weakening Fn invasion into tumor cells provides a new therapeutic avenue for exacerbating CRC problems caused by intestinal pathogenic bacteria.

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Isolation, characterization, and antimicrobial evaluation of bacteriocin produced by lactic acid bacteria against Erwinia carotovora

Cited by 12 publications

References 17 publications

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

Soybean flour as a substrate to obtain Enterococcus durans bacteriocins

A new anti-colon cancer tumor pathway of Phenyllactic acid by reducing adhesion of Fusobacterium nucleatum

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