Transesterification synthesis of high-yield biodiesel from black soldier fly larvae by using the combination of Lipase Eversa Transform 2.0 and Lipase SMG1

Abstract

The larvae of black soldier fly (BSFL) are rich in lipids, and in the current study, BSFL was used to produce biodiesel by enzymatic transesterification with methanol. Biodiesel obtained by free lipase-catalyzed transesterification is always with side-reactions. To address the limitation of free lipase, for the first time, Lipase SMG1 and Lipase Eversa Transform 2.0 were combined to use as catalysts at the same time. The effects of different reaction conditions on the yield of biodiesel were investigated, including the type of enzyme, reaction temperature, and molar ratio of BSFL to methanol. The molar ratio of BSFL to methanol 1:3, one-step addition of methanol at 25 °C were selected as optimal conditions. The fatty acid methyl ester content achieved to 98.45% after 8h reaction under the optimal conditions. Moreover, the main properties of the final biodiesel indicators met the EN14214 biodiesel standard.

Cultivo De Larvas De Mosca Soldado Negro Utilizando Resíduos Alimentares Para a Produção De Biodiesel: Revisão Sistemática Da Literatura

“…Os trabalhos selecionados têm métodos diferentes para a produção de biodiesel, como a transesterificação, solventes, catalisadores e entre outras formas (HE, 2022;TANG, 2022;NGUYEN, 2020). Estes métodos mencionados têm a função de tentar produzir o biodiesel com a melhor qualidade possível, assim com a utilização desses mecanismos químicos consegue-se precisar a extração dos lipídios, e dessa forma transformando em biodiesel.…”

The isolation and characterization of lipid compounds from black soldier fly larvae

“…Through a process known as transesterification, which entails the reaction of triglycerides (fat/oil) with alcohol, leading to the formation of esters and glycerol it is possible to convert the fat/oil extracted from these insects into biodiesel. [ 9,10,20 ] A relevant study conducted by He et al. [ 10 ] explored the production of biodiesel from BSFL using enzymatic transesterification with lipase SMG1 and lipase eversa transform 2.0 as catalysts.…”

“…[ 9,10,20 ] A relevant study conducted by He et al. [ 10 ] explored the production of biodiesel from BSFL using enzymatic transesterification with lipase SMG1 and lipase eversa transform 2.0 as catalysts. The results of their investigation demonstrated that employing a combination of lipase SMG1 and lipase eversa Transform 2.0 as catalysts led to a significantly higher yield of biodiesel compared to the use of a single enzyme.…”

“…Black soldier fly larvae (BSFL) have shown promise as an efficient source of renewable energy in addition to their capacity to control waste. [8][9][10][11] Due to overpopulation and the low popularity of renewable energy sources, the world is currently threatened by an impending energy crisis. [12][13][14][15] According to scientific evidence, nonrenewable conventional energy sources like fossil fuels and nuclear materials are gradually being used up and are anticipated to run out soon.…”

“…Through a process known as transesterification, which entails the reaction of triglycerides (fat/oil) with alcohol, leading to the formation of esters and glycerol it is possible to convert the fat/oil extracted from these insects into biodiesel. [9,10,20] A relevant study conducted by He et al [10] explored the production of biodiesel from BSFL using enzymatic transesterification with lipase SMG1 and lipase eversa transform 2.0 as catalysts. The results of their investigation demonstrated that employing a combination of lipase SMG1 and lipase eversa Transform 2.0 as catalysts led to a significantly higher yield of biodiesel compared to the use of a single enzyme.…”