Production of a novel probiotic date juice with anti-proliferative activity against Hep-2 cancer cells

Abstract

Date fruit is the ancient discovered and consumed fruit. Worldwide, Egypt is the largest producer of date. Besides its health benefits, many recent studies have proved its antioxidant, anticancer and anti-inflammatory effect. Currently, probiotic fermented fruit juices have a great attention especially for lactose intolerance patients. This study assessed the survival of two probiotics (Lactobacillus acidophilus and L. sakei) in the date juice. Phenolics characterization was performed by HPLC. Anti-proliferative activity of fermented juice against Caco-2 and Hep-2 cell lines was also evaluated. Under cold storage, L. sakei was still live for 2 weeks at 1.8 x 10 7 CFU/mL. In contrast, L. acidophilus reduced from 5.2 x 10 6 CFU/mL to below the minimum value of probiotic products just after 1 week. L. sakie significantly multiplied the total phenols and antioxidant activity in the juice compared to the unfermented juice. Interestingly, probiotic date juice exhibited an antitumor activity against larynx cell lines (Hep-2) with no activity against Caco-2. Date juice was proved to be suitable for probiotic and as potential larynx anticancer juice. Therefore, the developed juice is recommended to contribute to the available probiotic foodstuffs.

Extending the Shelf-Life of Fresh-Cut Green Bean Pods by Ethanol, Ascorbic Acid, and Essential Oils

“…The total sugar content was determined by the anthrone method at 630 nm as described in [ 28 ]. Briefly, 200 mg fruit were extracted three times with ethanol (80%) at 80 °C.…”

“…The total sugar content was determined by the anthrone method at 630 nm as described in [28]. Briefly, 200 mg fruit were extracted three times with ethanol (80%) at 80 • C. Then, the extracts were evaporated to dryness and redissolved in 2 mL distilled water.…”

Encapsulated Food Products as a Strategy to Strengthen Immunity Against COVID-19

“…Total polyphenolic content and antioxidant activity were increased during storage in juice containing L. sakie strains. Probiotic date juice showed an anti-tumor response against the larynx Hep-2 cell linen with no response against Caco-2 cell linen (192).…”

“…Moreover, these fruit juices can be used as immunity boosters to avoid viral diseases such as COVID-19 (122). Recently, Mostafa et al (192) developed the date juice containing L. sakei and L. acidophilus and examined the anti-proliferative activity against Hep-2 and Caco-2 cell lines. Total polyphenolic content and antioxidant activity were increased during storage in juice containing L. sakie strains.…”

Probiotic lactobacilli: Can be a remediating supplement for pandemic COVID-19. A review

“…The regular intake of probiotic fiber-rich dates fruits and dates kernel and other food strengthened their innate immunity and antioxidant status ( Guardole et al, 2016 ). Several studies have proved that the dates fruit and its extracts stimulate probiotic lactobacilli ( Al-Thubiani and Ahmed Khan, 2017 , Mustafa et al, 2020 ). The consumption of date palm fruits enhances intestinal microflora and increases bowel movements.…”