Avocado oil as an inducer of the extracellular lipase activity of Kluyveromyces marxianus L-2029

Martínez-Corona, Ricardo; Banderas-Martínez, Francisco Javier; Pérez-Castillo, Jessica Nyx; Cortés-Penagos, Carlos; González‐Hernández, Juan Carlos

doi:10.1590/fst.06519

Cited by 10 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…K. marxianus L-2029 is the KmYJR107Wp lipase(Martínez-Corona et al, 2019).As expected, the KmLIP3p secondary structure is highly hydrophobic, which can be related to the phenomenon of interfacial activation(Casas-Godoy et al, 2009). The position of potential disulfide bridge sites was not recognized in this structure.Hydrolase domains were also recognized in the amino acid sequence of the KmYJR107Wp lipase; DomPred predicted 13 domain sequences, although the alignment of the secondary structure (Figure 5b) of the predicted KmYJR107Wp model, with the YlLip2p lipase from Y. lipolytica determined the presence of nine hydrolase domains (data not shown).…”

mentioning

confidence: 70%

“…However, no interaction of this amino acid was observed with any histidine residue in the structure (Figure b), which would be part of the catalytic triad; it is likely that the putative protein encoded by the LIP3 gene is not actually a lipase and may have some other activity. As mentioned above, we have recognized that the only enzyme responsible for the lipase activity of K. marxianus L‐2029 is the KmYJR107Wp lipase (Martínez‐Corona et al, ).…”

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus

Martínez-Corona

Vázquez-Marrufo

Penagos

et al. 2019

Yeast

View full text Add to dashboard Cite

Lipases are hydrolytic enzymes that break the ester bonds of triglycerides, generating free fatty acids and glycerol. Extracellular lipase activity has been reported for the nonconventional yeast Kluyveromyces marxianus, grown in olive oil as a substrate, and the presence of at least eight putative lipases has been detected in its genome.However, to date, there is no experimental evidence on the physiological role of the putative lipases nor their structural and catalytic properties. In this study, a bioinformatic analysis of the genes of the putative lipases from K. marxianus L-2029 was performed, particularly identifying and characterizing the extracellular expected enzymes, due to their biotechnological relevance. The amino acid sequence of 10 putative lipases, obtained by in silico translation, ranged between 389 and 773 amino acids. Two of the analysed putative proteins showed a signal peptide, 25 and 33 amino acids long for KmYJR107Wp and KmLIP3p, and a molecular weight of 44.53 and 58.23 kDa, respectively. The amino acid alignment of KmLIP3p and KmYJR107Wp with the crystallized lipases from a patatin and the YlLip2 lipase from Yarrowia lipolytica, respectively, revealed the presence of the hydrolase characteristic motifs. From the 3D models of putative extracellular K. marxianus L-2029 lipases, the conserved pentapeptide of each was determined, being GTSMG for KmLIP3p and GHSLG for KmYJR107Wp; besides, the genes of these two enzymes (LIP3 and YJR107W) are apparently regulated by oleate response elements. The phylogenetic analysis of all K. marxianus lipases revealed evolutionary affinities with lipases from abH15.03, abH23.01, and abH23.02 families. K E Y W O R D S bioinformatic tools, Kluyveromyces marxianus, lipases, phylogeny

show abstract

mentioning

confidence: 70%

Section: Resultsmentioning

confidence: 97%

Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus

Martínez-Corona

Vázquez-Marrufo

Penagos

et al. 2019

Yeast

View full text Add to dashboard Cite

show abstract

“…Effect of concentration of avocado oil to the induction of lipases was also studied by Martínez-Corona et al [2019]. The strain Kluyveromyces marxianus was used in the cultivation.…”

Section: Resultsmentioning

confidence: 99%

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Małajowicz

Rochalska

Wacławek

2020

Zeszyty Problemowe Postępów Nauk Rolniczych

View full text Add to dashboard Cite

“…For determining the best avocado oil levels in the yeast medium, induction was performed for 24 h using different concentrations. K. marxianus L-2029 accumulated the highest productivity of extracellular lipase at 3.5% v/v avocado oil, pH 6, and 36°C ( Martinez-Corona et al, 2019 ). In another study, the same authors carried out a bioinformatics analysis of genes from K. marxianus L-2029 for the identification and characterization of extracellular lipases.…”

Section: Applications In Biotechnological Productsmentioning

confidence: 99%

Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology

Bilal

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Kluyveromyces marxianus is an emerging non-conventional food-grade yeast that is generally isolated from diverse habitats, like kefir grain, fermented dairy products, sugar industry sewage, plants, and sisal leaves. A unique set of beneficial traits, such as fastest growth, thermotolerance, and broad substrate spectrum (i.e., hemi-cellulose hydrolysates, xylose, l-arabinose, d-mannose, galactose, maltose, sugar syrup molasses, cellobiose, and dairy industry) makes this yeast a particularly attractive host for applications in a variety of food and biotechnology industries. In contrast to Saccharomyces cerevisiae, most of the K. marxianus strains are apparently Crabtree-negative or having aerobic-respiring characteristics, and unlikely to endure aerobic alcoholic fermentation. This is a desirable phenotype for the large-scale biosynthesis of products associated with biomass formation because the formation of ethanol as an undesirable byproduct can be evaded under aerobic conditions. Herein, we discuss the current insight into the potential applications of K. marxianus as a robust yeast cell factory to produce various industrially pertinent enzymes, bioethanol, cell proteins, probiotic, fructose, and fructo-oligosaccharides, and vaccines, with excellent natural features. Moreover, the biotechnological improvement and development of new biotechnological tools, particularly CRISPR–Cas9-assisted precise genome editing in K. marxianus are delineated. Lastly, the ongoing challenges, concluding remarks, and future prospects for expanding the scope of K. marxianus utilization in modern biotechnology, food, feed, and pharmaceutical industries are also thoroughly vetted. In conclusion, it is critical to apprehend knowledge gaps around genes, metabolic pathways, key enzymes, and regulation for gaining a complete insight into the mechanism for producing relevant metabolites by K. marxianus.

show abstract

Avocado oil as an inducer of the extracellular lipase activity of Kluyveromyces marxianus L-2029

Cited by 10 publications

References 37 publications

Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus

Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus

Effect of Avocado Oil on The Induction of Yeast Hydrolases

Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology

Contact Info

Product

Resources

About