Mulberry leaf polysaccharide extracted by response surface methodolog suppresses the proliferation, invasion and migration of MCF-7 breast cancer cells

Abstract

Breast cancer with highly heterogeneous characteristics is the main cause of cancer deaths in women all over the world. Chemotherapy-assisted diet therapy has become an effective way to improve the quality of life of cancer patients. Currently numerous dietary components have been found to inhibit the molecular events and signalling pathways associated with various stages of breast cancer development, but the dietary components extraction and its regulation mechanism were long-term and complex. In this study, low-temperature ultrasonic-assisted method was used to extract MLP, and through employing the Design-Expert software, the optimal conditions for MLP extraction were ultrasonic power 179.34 W, ultrasonic time 13.92 min, and ratio (v/w, mL/g) of water to raw material 23.55. Subsequent experiments evaluated the antioxidant activity of mulberry leaf polysaccharide and found that it has obvious ability to scavenge DPPH free radicals. Interestingly, the extracted MLP could inhibit the cell viability, migration and invasion of breast cancer cells in vitro. Therefore, we suggested MLP could been used as an antioxidant supplement that contains these micronutrients for cancer patients or used to develop anti-oxidant functional health food for breast cancer patients.

Extraction, purification, structural modification, activities and application of polysaccharides from different parts of mulberry

“…The polysaccharides from mulberry leaves exhibit antitumor activity. According to Feng's experiments, 73 when breast cancer cells were treated with varying concentrations of MLPs, MLPs were discovered to be able to lessen proliferation, migration, and invasion of breast cancer cells in a way that depends on both time and focus. This suggests that MLPs have the potential as a antioxidant-based health food for breast cancer patients.…”

Optimization of ultrasonic-assisted extraction of polysaccharide from fig leaves and its antioxidant activity

“…The response surface plot is the imagic display of the regression equation (Feng et al, 2022). As shown in Figure 2, the more significant the interaction between the factors, the greater the slope of the response surface.…”

Effects of Cirsium setidens extract on gene expression related to apoptosis induction and metastasis inhibition in human breast cancer

“…In addition, because it is classified as an invasive cancer with a recurrence rate of 30% or higher, early diagnosis, prevention, and treatment are necessary to prevent complications such as lymphangitis and ulcers (van der Leij et al, 2012;Lee et al, 2021). Although the viability rate of breast cancer is very high at 93.3% with chemotherapy, currently applied chemotherapy has side effects including venous thrombosis; thus safer, and more effective breast cancer prevention and treatments based on natural compound are necessary (Heo & Heo, 2021;Feng et al, 2022).…”