Extraction of echinacoside from Cistanche tubulosa (Schenk) R. Wight and investigation of its protective effect on liver injury in sepsis rats

Abstract

Materials and methods Extraction of echinacoside from Cistanche tubulosa (Schenk) R. WightAbout 1 kg dried fleshy stems of Cistanche tubulosa (Schenk) R. Wight was taken, and was crushed to obtain the powder. The powder was extracted with 10 times (volume to mass) of 70% ethanol by refluxing for two times, 2 h of each time. After filtering, the filtrate was obtained. The filtrates of two extractions were combined, followed by concentrating under reduced pressure. The concentrated solution was loaded to HP-20 adsorption resin column. The resin column was eluted with water until there is no sugar reaction, and then eluted with 30% ethanol. The target elution solution was obtained. After concentrating under reduced pressure and drying, the crude echinacoside product was taken. After purifying by silica-gel column chromatography, the final echinacoside product was obtained. The high-performance liquid chromatograph showed that the purity of echinacoside was 95.49%.