Experimental model for controlled endoscopic subepithelial vocal fold injury in rats

Ujvary, Laszlo Peter; Blebea, Cristina Maria; Dindelegan, Maximilian George; Ţiple, Cristina; Sevastre, Bogdan; Maniu, Alma; Chirilă, Magdalena; Cosgarea, Marcel

doi:10.1590/acb370106

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…The rat stands out as being the leading experimental model to date due to the vast amount of research data already available. Also, the availability of genetic sequencing and established surgical applications combined with low maintenance and rapid growing rates, make them the ideal candidate for experimental research (Ujvary et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Relevant Anatomical Details in Rats Regarding Otologic Research

Blebea¹,

Dindelegan²,

Ujvary³

et al. 2022

Int. J. Morphol.

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The objective of the study was to provide a detailed anatomical description of the rat's ear anatomy that will prove insightful to different experimental otologic surgical procedures regardless of scope. Three male Wistar rats were enrolled in the study. Candidates were screened for systemic and otologic pathology. External ear canal endoscopy was carried out with a 30˚ rigid endoscope through an image capture system. Middle ear anatomical elements were analyzed under stereomicroscopy. 3D computer tomography image reconstruction was realized with a micro-CT to describe the anatomy. Image data from all three rats were analyzed. Anatomical annotations and surgical exposure recommendations were added for key elements. The most relevant images from all three rats were selected for representation. Detailed visualization of the structural elements of the tympanic cavity were clearly visible: promontory, round window, stapedial artery, stapes, incus, and tympanic membrane were all constant findings. We describe a step wise ventral surgical approach of the middle and inner ear for which we found that the clavotrapezius muscle was a reliable landmark. For the transtympanic approach the endoscopic transcanal access was an easy and reliable method for which a detailed anatomical representation was depicted. Further, anatomical similarities to humans were observed by stereomicroscopy and Micro-CT imaging reiterating that the rat model is suitable for otologic research. The endoscopic approach to the tympanic membrane is comfortable and less expensive than a microscope. The tendon of the clavotrapezius muscle can be a reliable landmark for discovering the tympanic bulla when considering a ventral approach. 3D Micro-CT reconstruction allows intact evaluation of the samples, simultaneously being a diagnostic and also a learning tool.

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Section: Introductionmentioning

confidence: 99%

Relevant Anatomical Details in Rats Regarding Otologic Research

Blebea¹,

Dindelegan²,

Ujvary³

et al. 2022

Int. J. Morphol.

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Pulsed exposure mode of the 445 nm semiconductor laser in phonosurgery: an experimental study

Ryabova,

Mitrofanova,

Ulupov

et al. 2023

Biomedical photonics

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The study presents the results of an experimental study devoted to the choice of the most optimal mode of pulsed contact laser exposure of semiconductor laser with a wavelength of 445 nm in phonosurgery, which implies maximum preservation of anatomically and functionally significant structures of the larynx combined with a radical approach to the pathological process. From the standpoint of the mucoundular theory of voice formation, wave-like oscillations of the vocal folds are possible due to the mobility of the cover layer of the vocal fold (epithelium, superficial layer of the lamina propria) relative to its body (deep layer of the lamina propria, vocal muscle). Thus, any injury at the level of the integumentary layer is associated with the risk of excessive scarring and loss of the ability to wave-like sliding. Pig vocal folds, according to a number of authors, have a structure similar to human ones in terms of both histological structure and acoustic parameters, which justifies the rationality of their use as an experimental model. In a series of experiments using a 445 nm laser, contact pulsed impacts on a biological model were carried out with pulse durations of 10, 20, 50, and 100 ms, followed by evaluation of the following parameters based on the data of histological sections: the depth and width of the ablation crater, the width of the zone of lateral thermal damage. Thus, the most optimal for phonosurgical interventions modes of pulsed laser exposures with a wavelength of 445 nm are described.

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Experimental model for controlled endoscopic subepithelial vocal fold injury in rats

Cited by 2 publications

References 22 publications

Relevant Anatomical Details in Rats Regarding Otologic Research

Relevant Anatomical Details in Rats Regarding Otologic Research

Pulsed exposure mode of the 445 nm semiconductor laser in phonosurgery: an experimental study

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