Nova classificação dos carcinomas da mama: procurando o luminal A

Serra, Kátia Piton; Ramalho, Susana; Torresan, Renato Zocchio; Vassallo, José; Sarian, Luís Otávio; Silva, Geisilene Russano de Paiva; Derchain, Sophie

doi:10.1590/so100-720320140005158

Cited by 18 publications

(14 citation statements)

References 9 publications

(11 reference statements)

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“…Breast cancer, the cancer that originates in the breast tissues, is divided into five types based on its molecular characteristics: Luminal A, Luminal B, HER2 enriched, basal like/triple-negative, and other special types of breast cancer [ 23 ]. The response to treatment and prognosis varies with the type of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors

Jiao

Wang

Lü

et al. 2020

Stem Cells International

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Mesenchymal stem cells (MSCs) are being widely investigated for the development of novel therapeutic approaches for different cancers, including breast cancer, the leading form of cancer in women. Our previous study showed that the factors secreted by human umbilical cord MSCs (hUCMSCs) induced pyroptosis in the breast cancer cell line MCF7 and our RNA sequencing studies revealed an increase in the expression of the pyroptosis-related gene caspase-4 (CASP4) and nucleotide-binding, leucine-rich repeat pyrin domain-containing protein 1 (NLRP1) in pyroptotic MCF7 cells. Cellular pyroptosis can occur via the canonical pathway (involving caspase-1 and NLRP1) or the noncanonical pathway (involving caspase-4). In this study, we first confirmed that the inflammasome complex formed by NLRP1 and ASC is involved in MCF7 cell pyroptosis induced by hUCMSC-CM. Further, we investigated the role of CASP4 and NLRP1 in MCF7 cell pyroptosis induced by hUCMSC-secreted factors using shRNA-mediated transfection of CASP4 or NLRP1 in MCF7 cells. Cytotoxicity analyses revealed that neither CASP4 knockdown nor NLRP1 knockdown could inhibit the hUCMSC-CM-induced pyroptosis in MCF7 cells. Gene and protein expression analysis showed that hUCMSC-CM induced pyroptosis mainly via the canonical pathway in CASP4 knockdown MCF7 cells but mainly via the noncanonical pathway in NLRP1 knockdown MCF7 cells. Our study provides a foundation for further studies aimed at elucidating the precise mechanism underlying hUCMSC-induced pyroptosis in breast cancer cells and aid the identification of potential therapeutic targets for breast cancer.

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Section: Discussionmentioning

confidence: 99%

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors

Jiao

Wang

Lü

et al. 2020

Stem Cells International

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“…A partir da década de 90, o tratamento cirúrgico da mama passou de abordagens radicais para procedimentos menos extremos e conservadores da mama, sendo a cirurgia conservadora da mama, também chamada de lumpectomia, acompanhada de radioterapia um tratamento equivalente à mastectomia (TIEZZI, 2007;VERONESI et al, 1990). Porém, recentemente, tem havido uma tendência no aumento das taxas de mastectomia por razões desconhecidas (MCGUIRE et al, 2009 (UNTCH et al, 2015), (SERRA et al, 2014).…”

Section: Cirurgiaunclassified

Principais Tratamentos Utilizados No Combate Ao Câncer De Mama: Uma Revisão De Literatura

Nascimento

Mello²,

Schneider³

et al. 2019

Arq Mudi

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Resumo O câncer de mama é o mais frequente diagnóstico de câncer entre mulheres. Muito progresso tem sido alcançado nos últimos anos em relação ao tratamento, com diagnósticos cada vez mais precoces e precisos. O principal tratamento é a cirurgia, porém tratamentos adjuvantes como a quimioterapia, radioterapia, terapia hormonal e a imunoterapia reduzem o risco de recidiva do câncer de mama e o risco de morte por essa doença. Geralmente, estas terapias são indicadas após a cirurgia, como um complemento ao tratamento. Este trabalho tem como objetivo apresentar uma revisão de literatura sobre os principais métodos de tratamento do câncer de mama, suas implicações e resultados.

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“…According to variations in the expressions of the estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), breast cancer were defined as four major intrinsic molecular subtypes: luminal A, luminal B, HER2-positive and triple-negative breast cancer (TNBC) (Sorlie et al 2001). TNBC is characterized by a lack of expression of the ER and PR as well as HER2 (Serra et al 2014). TNBC that occurs mostly in premenopausal young women represents approximately 15-20% of all invasive breast cancers (Foulkes et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer (v0.2)"

2020

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Background. Breast cancer is a heterogeneous disease. Compared with other subtypes of breast cancer, triple-negative breast cancer (TNBC) is easy to metastasize and has a short survival time, less choice of treatment options. Here, we aimed to identify the potential biomarkers to TNBC diagnosis and prognosis. Material/Methods. Three independent data sets (GSE45827, GSE38959, GSE65194) were downloaded from the Gene Expression Omnibus (GEO). The R software packages were used to integrate the gene profiles and identify differentially expressed genes (DEGs). A variety of bioinformatics tools were used to explore the hub genes, including the DAVID database, STRING database and Cytoscape software. Reverse transcription quantitative PCR (RT-qPCR) was used to verify the hub genes in 14 pairs of TNBC paired tissues. Results. In this study, we screened out 161 DEGs between 222 non-TNBC and 126 TNBC samples, of which 105 genes were up-regulated and 56 were down-regulated. These DEGs were enriched for 27 GO terms and 2 pathways. GO analysis enriched mainly in "cell division", "chromosome, centromeric region" and "microtubule motor activity". KEGG pathway analysis enriched mostly in "Cell cycle" and "Oocyte meiosis". PPI network was constructed and then 10 top hub genes were screened. According to the analysis results of the Kaplan-M eier survival curve, the expression levels of only NUF2, FAM83D and CENPH were associated with the recurrence-free survival in TNBC samples (P<0.05). RT-qPCR confirmed that the expression levels of NUF2 and FAM83D in TNBC tissues were indeed up-regulated significantly. Conclusions. The comprehensive analysis showed that NUF2 and FAM83D could be used as potential biomarkers for diagnosis and prognosis of TNBC.

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Nova classificação dos carcinomas da mama: procurando o luminal A

Abstract: The 2013 St. Gallen consensus updated the definition of intrinsic molecular subtypes and increased the number of patients classified as having luminal B-like breast cancer in our series, for whom the use of cytotoxic drugs will probably be proposed with additional treatment cost.

Cited by 18 publications

References 9 publications

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors

Principais Tratamentos Utilizados No Combate Ao Câncer De Mama: Uma Revisão De Literatura

Peer Review #2 of "Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer (v0.2)"

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