Estudios epidérmicos en helechos y licófitas medicinales de la Provincia de Córdoba, Argentina

Abstract:Background and Aims: Anemiaceae has a single genus, Anemia, with 115 species distributed in the Neotropics, Africa, India, and islands in the Indian Ocean. The aim of this work is characterize living spores and gametophytic phase of three Anemia taxa from South America, little studied or with fragmentary studies, A. herzogii, A. tomentosa var. anthriscifolia and A. tomentosa var. tomentosa.Methods: Gametophytes of the three taxa have been obtained through in vitro cultures of spores. The spores and gametophytes were studied with light and scanning electron microscopes. The germination pattern of the spores, the evolution of the gametophytic development and the sexual expression were described.Key results: Filamentous gametophytes were formed by 4-20 cells, the adults were cordate, symmetrical or asymmetrical, bisexual and protandric, on their surfaces and margins clavate trichomes were observed. Gametangia corresponded to the leptosporangiate type. The dehiscence of antheridia was produced by a perforation in the opercular cell. Archegonia had necks with four to seven cells and four triangular cells at the distal end of the neck. Anemia herzogii showed branched trichomes on the gametophytes and later developed sporophytes.Conclusions: Germination in all taxa corresponds to the Anemia-type and the gametophytic development to the Ceratopteris-type. Anemia herzogii is distinguished from the two varieties by the presence of branched trichomes exclusive of the gametophytic phase. The studied taxa are characterized by the morphology of gametophyte, type of trichomes and sexuality.

show abstract

“…Sm. (Luján et al, 2011). The type of stomata observed in the studied species coincide with those of Luján et al (2011).…”

Section: Discussionsupporting

confidence: 71%

Fase gametofítica de tres taxones de Anemia (Anemiaceae)

Chambi

Martínez

2020

Acta Bot. Mex.

View full text Add to dashboard Cite

show abstract

“…Regarding the trichomes of the lamina, we observed three types of glandular trichomes: naviculate, unicellular, called clavate by Roux et al (1992), and uniseriate multicellular with terminal gland. The latter authors also consider naviculate and clavate trichomes as glandular in species of the genus Mohria Sw., whereas Luján et al (2011) described unicellular trichomes as eglandular in Anemia australis. The multicellular uniseriate glandular trichome described in this research agrees with the findings of Roux et al (1992) and Luján et al (2011).…”

Section: Discussionmentioning

confidence: 99%

“…The latter authors also consider naviculate and clavate trichomes as glandular in species of the genus Mohria Sw., whereas Luján et al (2011) described unicellular trichomes as eglandular in Anemia australis. The multicellular uniseriate glandular trichome described in this research agrees with the findings of Roux et al (1992) and Luján et al (2011). The petiole showed two types of glandular trichomes: unicellular to bicellular foot with unicellular head, and uniseriate multicellular with terminal gland.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anatomía del esporofito de Anemia phyllitidis var. phyllitidis (Anemiaceae) de un bosque de ribera (Tucumán, Argentina)

2021

View full text Add to dashboard Cite

Background and Aims: In Argentina seven species of the genus Anemia are recognized; one of them, A. phyllitidis, has two varieties. The anatomical records for A. phyllitidis var. phyllitidis are scarce and refer to the ontogeny of stomata, epidermis structure and type of petiole stele. This variety, which occurs in riparian forests, is affected by the increase of seasonal ecological pressures. There is scarce information about its sporophyte anatomy; hence, the aim of this work was to characterize it.Methods: Five specimens were collected from the margin of the El Parque stream (Tucumán-Argentina). A part was herborized and the other part was fixed in FAA. Subsequently, conventional histological techniques were used and stoma density and size of stomata and trichomes were determined. Histochemical tests were performed to detect starch (lugol) and phenolic compounds (10% ferric chloride).Key results: Anemia phyllitidis var. phyllitidis shows diarch roots and dictyostelic rhizome covered with glandular trichomes. The petiole is covered by glandular trichomes, epidermis and subepidermis composed of lignified fibers, vascular bundle with phloem surrounding xylem, two-layered pericycle surrounded by endodermis and cortical tissue with starch and phenolic compounds. Sterile pinnae have pericytic, anomocytic, and desmocytic stomata (mean size 49.8 µm × 38.7 µm); glandular trichomes; dorsoventral hypostomatic lamina, vascular bundles with pericycle and endodermis. Fertile pinnae are anatomically similar to the rachis. Silica is present in some epidermal cells of the different organs.Conclusions: The sporophyte of A. phyllitidis var. phyllitidis showed anatomical, morphological and physiological traits that reveal its adaptation to the riparian habitat where it grows. At the same time, the description of this variety is deepened and unpublished data for the genus are provided, such as the presence of stegmata in the fertile pinnae.

show abstract

Micro morphological foliar taxonomic studies of genus Paspalum based on scanning electron microscopy

Shaheen

Sharifi‐Rad

Hussain

et al. 2021

Microscopy Res & Technique

View full text Add to dashboard Cite

Paspalum is one of the 10 largest genera in Poaceae and is considered as a complex and taxonomically difficult genus due to its similar morphological characters within the species which makes it difficult to delimit taxa within the same genus. The current research work establishes the distinction among species of this genus and resolves taxonomic confusions among this genus. And this distinction is done by micro morphological foliar anatomical characterization by light and scanning electron microscopy. Both the abaxial and adaxial epidermal cuticles of three different species of genus Paspalum were studied and their phytoliths were observed through elemental dispersive spectrophotometric analysis by SEM first time in Pakistan. Results showed great variation in studied anatomical features. P. dilatatum Poir. was comprised of macro-hairs in the intercostal regions only whereas no macro-hairs were found in P. distichum Linn. and P. scrobiculatum Linn. Beak-shaped prickles were found in the intercostal regions on adaxial surface only in P. distichum Linn. and P. scrobiculatum Linn. whereas in P. dilatatum Poir., knife-shaped prickles were present in intercostal regions only on both abaxial and adaxial surfaces of leaf epidermis. No papillae were seen in P. dilatatum Poir whereas in P. distichum Linn. and P. scrobiculatum Linn. papillae were found abundantly. In genus paspalum, P. dilatatum Poir. phytoliths showed lowest mass percentage of silicon as 15.13% whereas P. distichum Linn. phytoliths showed highest mass percentage of silicon as 22.00%. Based on micro morphological foliar characteristics a taxonomic key of studied species is also provided to delimit the taxa.

show abstract

Estudios epidérmicos en helechos y licófitas medicinales de la Provincia de Córdoba, Argentina

Cited by 5 publications

References 21 publications

Fase gametofítica de tres taxones de Anemia (Anemiaceae)

Fase gametofítica de tres taxones de Anemia (Anemiaceae)

Anatomía del esporofito de Anemia phyllitidis var. phyllitidis (Anemiaceae) de un bosque de ribera (Tucumán, Argentina)

Micro morphological foliar taxonomic studies of genus Paspalum based on scanning electron microscopy

Contact Info

Product

Resources

About