Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte

Abstract

Cite as: Sipaúba-Tavares, L.H. et al. Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte. Acta Limnologica Brasiliensia, 2017, vol. 29, e5. Abstract: Aim:The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods: Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture medium to compare the growth of microalgae and commercial medium CHU 12 . Protein and lipid contents, water conditions of the culture medium and physiological parameters were assessed weekly. Results: Growth rate, macro-minerals, micro-nutrients and nutritional values were similar to commercial culture medium in the media with inorganic fertilizer and macrophyte. The biological indexes of microalgae were not influenced by weekly differences during the study period. Although lipid contents, nitrogen and growth rate of A. gracilis were higher in inorganic fertilizer and macrophyte media; Mn, B, Cu, S, dry matter, ash content, cell volume, TOC and total length were similar among the media used. The same high cell density 25 x 10 5 cells.mL -1 was obtained in NPK and in CHU 12 medium respectively after 11 and 14 days cultivation. Conclusion: Culture media inorganic fertilizer (NPK) and macrophyte (Eichhornia crassipes), were adequate and may replace the commercial medium CHU 12 for the cultivation of microalgae A. gracilis, resulting in high nutritional composition, high biomass, high nutrients and low-cost.

Commercial Microalgae Culture in Inorganic Fertilizer Media

“…Higher cell biomass and longer stationary phase were observed in the composite and the BG11 media, but there was no statistically significant difference among the media. This result agrees with [30] who observed that NPK fertilizer and macrophyte can supply adequate nutrients and may replace synthetic medium for large scale algae cultivation. Similarly, the study by [31] suggested that agricultural fertilizer can provide required nutritional support for algae growth though the Bristol medium has relatively higher cell biomass concentration when compared with fertilizer medium.…”

“…The dry cell biomass obtained at stationary phase from both NPK of 0.0176 mg/l and composite media of 0.0185 mg/L was close to the value of 0.0212 mg/L obtained from the BG11, which is a synthetic medium. Although the BG11 medium has been modified over the years with all the essential elements for optimal growth, this experiment has shown that little modification of the NPK medium will provide a relatively low cost and better nutrient medium for commercial algae biomass production as observed by [30,21]. When compared statistically to the significance level (P<0.05), there were no significant differences were found in cell dry weight between media studied.…”

Evaluation of the effects of sugarcane molasses as a carbon source for Ankistrodesmus gracilis and Haematococcus pluvialis (Chlorophyceae)

“…Scardoelli-Truzzi and Sipaúba-Tavares (2017) demonstrated that an increase in H. pluvialis biomass using 0.75 g L -1 of sugarcane molasses concentrations was higher than in phototrophic conditions with regard to cell volume, total length, TOC and dry weight. However, if chlorophyll-a content of A. gracilis in autotrophic cultivation (Sipaúba-Tavares et al, 2017) is compared with mixotrophic cultivation, the concentration is 10-fold higher in culture with sugarcane molasses as a carbon source. In mixotrophic cultivation light and organic carbon source fulfil the same role in the growth and cellular composition of microalgae (Piasecka et al, 2017).…”

“…The effect of light may be observed in current study when A. gracilis was cultured in 60 µmolm -2 s -1 lipid levels were less than 3.2%DW in NPK culture medium. The same microalga was cultured under the same conditions, but lipids levels were 11.5%, in 40 µmolm -2 s -1 light intensity (Sipaúba-Tavares et al, 2017).…”

Influence of agriculture fertilizer for the enhanced growth and astaxanthin production from Haematococcus lacustris RRGK isolated from Himachal Pradesh, India

“…Potassium is a macroelement which is required for growth-related metabolic activities [27]. The inorganic fertilizer is simple and may be used with alternative medium for microalgae, since they are widely available, dissolve easily, have a defined composition, high nitrogen and phosphorus rate, and maintain moderate pH in the medium [28,29]. The inorganic fertilizers such as NPK result in an efficient alternative medium for largescale laboratory growth of Chlorophyceaen members [30].…”