Changes in water quality and stream habitats can significantly impact Odonata larvae dynamics as a result of their specific ecological requirements. This study investigated the effects of water quality and microhabitats on the structure of Odonata larval communities. During the same period, substrate types, water variables, and Odonata larvae were sampled. Our findings reveal that substrate type had no impact on community structure. However, the presence of ammonia and oxygen levels, among other water quality, influenced the abundance of Odonata larvae. While Odonata organisms are sensitive to the local environment, the intermittent flow of the creek and seasonal changes may be responsible for the lack of substrate influence on the Odonata community. Abiotic factors also play a critical role since Odonata larvae have limits to which they can tolerate. Variations in these factors can have a profound impact on the persistence and survival of the larvae. Additionally, the larvae's physiological responses, such as respiration, are influenced by these factors. Zygoptera larvae, which have branchial respiration, necessitate a water environment with low temperatures and high levels of dissolved oxygen. Therefore, the abiotic variables of the stream significantly affect the Odonata larvae community. Incorporating natural environmental changes and variations into monitoring and conservation programs is essential. By doing so, we can enhance our understanding of biodiversity and its distribution patterns, leading to more effective preservation efforts.