Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

Abstract

Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L -1 , T3-tea 10 g.L -1 , T4-glyphosate at 9.6%, T5-glyphosate with subsequent recovery in distilled water, T6-glyphosate with subsequent recovery in tea 5 g.L -1 and T7-glyphosate with subsequent recovery in tea 10 g.L -1 . The rootlets were collected and fixed in ethanol:acetic acid (3:1) for 24 hours, then stored in 70% ethanol under refrigeration. Analysis was performed using high performance liquid chromatography for the quantification of the extracted phenolic compounds. Gallic acid, catechin, caffeic acid, and rutin were abundant in the extracts of P. granatum. The extracts were found to exhibit antiproliferative potential but not antimutagenic or genotoxic activity.Uniterms: Punica granatum L./phytochemistry. Punica granatum L./genotoxicity Punica granatum L./ chromatography analysis. Medicinal plants. Pomegranate.Punica granatum L., conhecida como romanzeira, é originária da Ásia e encontra-se distribuída por todo Brasil. É usada para o tratamento de doenças inflamatórias, infecciosas e respiratórias. Em decorrência da grande utilização de recursos fitoterápicos, é necessário esclarecer à população sobre a grande quantidade de substâncias existentes nas plantas e sobre os benefícios e prejuízos de tais substâncias à saúde. O presente trabalho objetivou realizar a análise cromatográfica e o estudo da genotoxicidade dos extratos aquosos das cascas dos frutos de P. granatum através do teste de Allium cepa L. Para a montagem do experimento, foram utilizados 7 tratamentos: T1-água destilada, T2-chá 5 g.L -1 , T3-chá 10 g.L -1 , T4-glifosato a 9,6%, T5-glifosato para recuperação em água destilada, T6-glifosato para recuperação em chá 5 g.L -1 e T7-glifosato para recuperação em chá 10 g.L -1 . As radículas foram coletadas e fixadas em etanol:ácido acético (3:1) por 24 horas, e armazenadas em álcool 70%, sob refrigeração. Realizouse análise por cromatografia líquida de alta eficiência para quantificação dos compostos fenólicos. Nos extratos de P. granatum foram observados em maior quantidade: ácido gálico, catequina, ácido cafeico e rutina. Além disso, os extratos demonstraram potencial antiproliferativo, sem apresentar atividade antimutagênica e genotóxica. (Braga, 1961) and is cultivated around the world in regions with tropical and subtropical climates. It is used as an ornamental plant, as a fruit, and for its medicinal properties (Corrêa, 1978). UnitermosAccording to Garcia (1992), the species has antiinflammatory and antibacterial properties. Naqyi et al. (1991) evaluated the antimicrobial activity of an extract of P. granatum on gram-positive bacteria and yeast, finding antimicrobial ac...

Study of viability of high pressure extract from pomegranate peel to improve carrot juice characteristics

“…bovine kidney epithelial cells and Food & Function Paper human cells), mice, [34][35][36] and Artemia salina, Allium cepa, and zebrafish toxicity tests. 12,37,38 Five of these studies found that PPE can have potential cyto-or genotoxic effects. Therefore, additional screening models with biological relevance should be used to determine the safety of PPE for food applications.…”

Effects of Allium cepa and Its Constituents on Respiratory and Allergic Disorders: A Comprehensive Review of Experimental and Clinical Evidence

“…The decrement of gold nanoparticles synthesized with onion and inoculation of this combination affected E. coli in trypticase soy broth. Application of this combination to E. coli and incubation for a period of time caused cell lysis, showing antibacterial effect of the combination; thus, onion could be an effective candidate for sanitation of food and healthcare institutions [ 105 ].…”

Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

“…The A. cepa test has been used to detect a variety of environmental pollutants in industrial wastewater and the results obtained were reported significant versus control (Leme and Marin-Morales, 2009). A number of studies have been performed using A. cepa test system and positive responses of A. cepa have been reported i.e., complex mixture (Aguiar et al, 2016;Andrade-Vieira et al, 2012;Blagojević et al, 2009;Chakraborty and Mukherjee, 2010;Chakraborty et al, 2009;Klauck et al, 2013;Mazzeo et al, 2011b;Pathiratne et al, 2015;Pekol et al, 2012;Vidaković et al, 1993;Yahaya et al, 2012), antitoxic agent (Aslantürk and Çelik, 2006;Frescura et al, 2012;Kuhn et al, 2015;Kumari et al, 2013;Majewska et al, 2003;Nantes et al, 2014;Oyeyemi and Bakare, 2013;Santana et al, 2016;Ślusarczyk et al, 2014;Ślusarczyk et al, 2016;Sultan and Çelik, 2009), contaminated soil (Christofoletti et al, 2013;Dash et al, 1988;Kataeva et al, 2012;Kopliku and Mesi, 2012b;Masood and Malik, 2013a;Meier et al, 1997;Oberholster et al, 2008;Paul et al, 2013;Saxena et al, 2004;Sayles et al, 1999), drugs (Firbas and Amon, 2014;Grisolia and Takahashi, 1991;Jos et al, 2003;…”