Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

Ferraz, Rafaela Siqueira; Mendonça, Elisangela A. M.; Silva, Jéssica Priscila Avelino; Cavalcanti, Isabella Macário Ferro; Nogueira, Mariane Cajubá de Britto Lira; Galdino, Suely Lins; Pitta, Ivan R.; Lima, Maria do Carmo Alves de; Santos-Magalhães, Nereide Stela

doi:10.1590/s1984-82502015000100018

Cited by 5 publications

(5 citation statements)

References 20 publications

(21 reference statements)

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“…In this study, the analytical UV-Vis spectrophotometric condition refers to the previously optimized and validated method as per the International Harmonisation (ICH) guideline [ 19 , 20 , 21 ]. In this study, we performed the specificity and linearity assessments to verify the suitability of the spectrophotometric condition for analyzing and quantifying ketoprofen, and the protocol has been described in Section Validation of the UV-Vis Spectrophotometer.…”

Section: Resultsmentioning

confidence: 99%

“…The calibration curve was obtained in the concentration range of 2, 4, 6, 8, 10, and 12 µg/mL of standard ketoprofen [ 59 ]. The analytical spectrophotometric condition used in this study refers to the previously optimized and validated method as per the International Council for Harmonisation (ICH) guideline [ 19 , 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The validation of a UV spectrophotometric analytical method was tested using several parameters, including specificity and linearity [ 20 , 21 ]. To evaluate the specificity of the method, the UV spectra of blank samples (PVA or PVP) were compared to a ketoprofen standard solution.…”

Section: Methodsmentioning

confidence: 99%

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Combination of Dissolving Microneedles with Nanosuspension and Co-Grinding for Transdermal Delivery of Ketoprofen

et al. 2023

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Ketoprofen is an anti-inflammatory agent that may cause gastric irritation if administered orally. Dissolving microneedles (DMN) can be a promising strategy to overcome this issue. However, ketoprofen has a low solubility; therefore, it is essential to enhance its solubility using certain methods, namely nanosuspension (NS) and co-grinding (CG). This research aimed to formulate DMN containing ketoprofen-loaded NS and CG. Ketoprofen NS was formulated with poly(vinyl alcohol) (PVA) at concentrations of 0.5%, 1%, and 2%. CG was prepared by grinding ketoprofen with PVA or poly(vinyl pyrrolidone) (PVP) at different drug–polymer ratios. The manufactured ketoprofen-loaded NS and CG were evaluated in terms of their dissolution profile. The most promising formulation from each system was then formulated into microneedles (MNs). The fabricated MNs were assessed in terms of their physical and chemical properties. An in vitro permeation study using Franz diffusion cells was also carried out. The most promising MN-NS and MN-CG formulations were F4-MN-NS (PVA 5%-PVP 10%), F5-MN-NS (PVA 5%-PVP 15%), F8-MN-CG (PVA 5%-PVP 15%), and F11-MN-CG (PVA 7.5%-PVP 15%), respectively. The cumulative amounts of drug permeated after 24 h for F5-MN-NS and F11-MN-CG were 3.88 ± 0.46 µg and 8.73 ± 1.40 µg, respectively. In conclusion, the combination of DMN with nanosuspension or a co-grinding system may be a promising strategy for delivering ketoprofen transdermally.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Combination of Dissolving Microneedles with Nanosuspension and Co-Grinding for Transdermal Delivery of Ketoprofen

et al. 2023

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“…Six samples of Aztreonam (1-6 µg/ml) in ALF and gamble solution were analyzed at three different time points. The % RSD was calculated for both the methods 10,11 .…”

Section: Determination Of Precisionmentioning

confidence: 99%

Untitled

2019

IJPSR

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Aztreonam, a synthetic beta-lactam antibiotic is widely used for the treatment of infections in lungs, meninges, bladder, etc. Its potential use in the treatment of pneumonia and cystic fibrosis has been established. Recently FDA has approved Cayston® for inhalation therapy of Aztreonam in lungs infection. Many studies have been reported on the quantitative determination of Aztreonam by UV spectrophotometric and HPLC method. The present study focuses on the quantitative spectrophotometric determination of Aztreonam in two simulated lungs fluid namely artificial lysosomal fluid (ALF) and gamble solution. The λ max was found at a wavelength of 293 nm for both the solutions. Further, the method developed was validated for its linearity, precision by interday and within day study, accuracy, specificity, robustness and determination of limit of quantification and limit of detection in both the solutions. The linearity demonstrated a correlation coefficient of 0.9995 and 0.9999 in ALF and gamble solution respectively. The LOD was found to be 0.38 µg/ml and 0.18 µg/ml for ALF and gamble solution respectively. The LOQ was found to be 1.15 µg/ml and 0.53 µg/ml for ALF and Gamble solution respectively. The proposed method was found to be simple, rapid, accurate, precise, and specific for the determination of Aztreonam in simulated lungs fluid.

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“…HPLC requires expensive operation and instrumentation, and time-consuming sample processing. Besides, it also requires experience in the handling of the equipment [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Biocatalysis for D-DIBOA Synthesis Using a Quick and Sensitive New Spectrophotometric Quantification Method

Cabrera

Linares

Calle

et al. 2020

IJMS

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D-DIBOA (4-hydroxy-(2H)-1,4-benzoxazin-3-(4H)-one) is an allelopathic-derived compound with interesting herbicidal, fungicidal, and insecticide properties whose production has been successfully achieved by biocatalysis using a genetically engineered Escherichia coli strain. However, improvement and scaling-up of this process are hampered by the current methodology for D-DIBOA quantification, which is based on high-performance liquid chromatographic (HPLC), a time-consuming technique that requires expensive equipment and the use of environmentally unsafe solvents. In this work, we established and validated a rapid, simple, and sensitive spectrophotometric method for the quantification of the D-DIBOA produced by whole-cell biocatalysis, with limits of detection and quantification of 0.0165 and 0.0501 µmol·mL−1 respectively. This analysis takes place in only a few seconds and can be carried out using 100 µL of the sample in a microtiter plate reader. We performed several whole-cell biocatalysis strategies to optimize the process by monitoring D-DIBOA production every hour to keep control of both precursor and D-DIBOA concentrations in the bioreactor. These experiments allowed increasing the D-DIBOA production from the previously reported 5.01 mM up to 7.17 mM (43% increase). This methodology will facilitate processes such as the optimization of the biocatalyst, the scaling up, and the downstream purification.

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Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

Cited by 5 publications

References 20 publications

Combination of Dissolving Microneedles with Nanosuspension and Co-Grinding for Transdermal Delivery of Ketoprofen

Combination of Dissolving Microneedles with Nanosuspension and Co-Grinding for Transdermal Delivery of Ketoprofen

Untitled

Optimization of the Biocatalysis for D-DIBOA Synthesis Using a Quick and Sensitive New Spectrophotometric Quantification Method

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