The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain

Novaes, Letícia Célia de Lencastre; Jozala, Ângela Faustino; Mazzola, Priscila Gava; Pessoa, Adalberto

doi:10.1590/s1984-82502014000200017

Cited by 16 publications

(6 citation statements)

References 20 publications

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“…Novaes and co‐workers studied the influence of pH, PEG and polyacrylic acid (PAA) on bromelain stability, and found that bromelain was stable at pH 5.0 for a longer period, at all temperatures studied (20, 30, 40, and 50°C). The polymers studied had the opposite effect: while PEG solutions increased bromelain stability, PAA solutions decreased it.…”

Section: Stabilitymentioning

confidence: 99%

“…These destabilizing effects of PEG suggest that care should be taken when using PEG for thiol protease and hydrophobic proteins in general, even at room temperature, during salting-out, crystallization, and industrial applications. Novaes and co-workers 26 studied the influence of pH, PEG and polyacrylic acid (PAA) on bromelain stability, and found that bromelain was stable at pH 5.0 for a longer period, at all temperatures studied (20,30,40, and 508C). The polymers studied had the opposite effect: while PEG solutions increased bromelain stability, PAA solutions decreased it.…”

Section: Stabilitymentioning

confidence: 99%

See 1 more Smart Citation

Stability, purification, and applications of bromelain: A review

Novaes

Jozala

Lopes

et al. 2015

Biotechnol Progress

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108

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Bromelain is a cysteine protease found in pineapple tissue. Because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid-liquid extractions by aqueous two-phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up-to-date compilation of the data reported on this enzyme.

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Section: Stabilitymentioning

confidence: 99%

Section: Stabilitymentioning

confidence: 99%

Stability, purification, and applications of bromelain: A review

Novaes

Jozala

Lopes

et al. 2015

Biotechnol Progress

Self Cite

108

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“…In another study, the bromelain extracted from pineapple stem lost only 0.2% of its initial activity, at pH 5, after 7 h at 40°C. In addition, 43.30% of the initial activity of the above enzyme was lost at pH 6 during the same period [81].…”

Section: Biochemical and Thermodynamic Properties Of The Partially Pu...mentioning

confidence: 94%

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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Macambira plant (Bromelia laciniosa Mart. Ex Shult. & Schult. f.) is a species belonging to the family Bromeliaceae, which is distributed widely in the hinterlands of northeastern Brazil, from Bahia to Piauí states. The plants of this species contain an enzyme complex named bromelain, which has great potential for therapeutic applications. In the present study, bromelain derived from Bromelia laciniosa leaves was assessed for its thermodynamic properties and in vitro cytotoxic and healing activities. Bromelain was extracted from the leaves and partitioned using ethanol. The partially purified bromelain was then characterized to determine the optimum pH and temperature and the kinetic and thermodynamic parameters. The partially purified bromelain derived from macambira leaves exhibited its highest activity at 47°C and pH 7, when the Km and Vmax values of 2.26 µM and 2.083 U/mL, respectively. The half-life of this bromelain at 47°C was 5.78 h. At 47°C, the Gibbs's free energy, enthalpy, and entropy values reached 89, 53.54, and –0.11 kJ/mol K, respectively. In the SDS-PAGE analysis, bromelain appeared as the main protein band, with an estimated molecular mass of 33 kDa. The in vitro cytotoxic activity and wound healing effects were determined using the MTT assay and Scratch assay, respectively. These assays were performed on the non-neoplastic cell lines L-929 and PBMC and the human tumor cell lines MCF-7, HCT-116, and NCI-H292. Bromelain exhibited a positive healing activity with a change rate similar to that observed for the control. In addition, bromelain exhibited cytotoxic activity against NCI-H292 tumor cells and chemopreventive potential. These results indicate that bromelain derived from macambira leaves may be utilized as a potential ingredient in pharmaceutical formulations, for various healing and chemopreventive purposes and as an alternative to the bromelain extracted from pineapple. In addition, the biochemical characteristics of this bromelain could find application in the food industry. Future studies should, therefore, focus on deciphering the action mechanisms underlying the activities of bromelain observed in the present study to further confirm and enhance its application potential for therapeutic purposes.

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“…Both stem and fruit bromelain are isolated as glycosylated, monomeric single proteins [2] with an isoelectric point (pI) value of 9.55 and optimum pH range of 6-7 [18]. The proteins also contain seven cysteine residues [19] and hence a total of three disulfide bonds.…”

Section: Structural Chemistry Of Bromelainmentioning

confidence: 99%

Bromelain, a Group of Pineapple Proteolytic Complex Enzymes (Ananas comosus) and Their Possible Therapeutic and Clinical Effects. A Summary

Varilla

Marcone

Paiva

et al. 2021

Foods

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Bromelain is a complex combination of multiple endopeptidases of thiol and other compounds derived from the pineapple fruit, stem and/or root. Fruit bromelain and stem bromelain are produced completely distinctly and comprise unique compounds of enzymes, and the descriptor “Bromelain” originally referred in actuality to stem bromelain. Due to the efficacy of oral administration in the body, as a safe phytotherapeutic medication, bromelain was commonly suited for patients due to lack of compromise in its peptidase efficacy and the absence of undesired side effects. Various in vivo and in vitro studies have shown that they are anti-edematous, anti-inflammatory, anti-cancerous, anti-thrombotic, fibrinolytic, and facilitate the death of apoptotic cells. The pharmacological properties of bromelain are, in part, related to its arachidonate cascade modulation, inhibition of platelet aggregation, such as interference with malignant cell growth; anti-inflammatory action; fibrinolytic activity; skin debridement properties, and reduction of the severe effects of SARS-Cov-2. In this paper, we concentrated primarily on the potential of bromelain’s important characteristics and meditative and therapeutic effects, along with the possible mechanism of action.

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The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain

Cited by 16 publications

References 20 publications

Stability, purification, and applications of bromelain: A review

Stability, purification, and applications of bromelain: A review

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Bromelain, a Group of Pineapple Proteolytic Complex Enzymes (Ananas comosus) and Their Possible Therapeutic and Clinical Effects. A Summary

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