High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil

Siqueira, Vera Lúcia Dias; Cardoso, Rosilene Fressatti; Pádua, Rubia Andréia Falleiros de; Caleffi-Ferracioli, Katiany Rizzieri; Helbel, César; Santos, Adolfo Carlos Barreto; Aoki, Elisabeth Eyko; Nakamura, Celso Vataru

doi:10.1590/s1984-82502013000100006

Cited by 4 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar study conducted by Siqueira et al . [14] found that small pocket groupings of Pseudomonas and Acinetobacter encoded with carbapenemase resistance genes were detected showing clonal similarities by the ERIC-PCR amplification technique in a Brazilian hospital [14]. Of note, these findings were comparable to those in this present study, in which close relatedness were found in 3 cluster-pair patterns of Acinetobacter baumannii isolates recovered from aspirate, wound and urine, and 2 cluster-pair Pseudomonas aeruginosa isolates from urine and wound specimens.…”

Section: Discussionsupporting

confidence: 87%

“…Many vulnerable patients, such as the elderly in ICUs, and children and babies admitted in NICU, are at an increased risk of infections caused by these organisms [29, 30]. An evidence based study carried out in paediatric and NICU wards recovered emerging carriers of OXA-type carbapenemase genes in Pseudomonas and Acinetobacter species, [14] while both species have been found as carriers of the MBL-type genes [31, 32].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique

et al. 2019

View full text Add to dashboard Cite

AimEnterobacterial repetitive intergenic consensus (ERIC) sequence analysis is a powerful tool for epidemiological analysis of bacterial species. This study aimed to determine the genetic relatedness or variability in carbapenem-resistant isolates by species using this technique.MethodsA total of 111 non-duplicated carbapenem-resistant (CR) Gram-negative bacilli isolates from a three-year collection period (2012–2014) were investigated by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC–PCR) in four selected hospital laboratories in Ghana. The isolates were also screened for carbapenemase and extended spectrum β-lactamase genes by PCR.ResultsA proportion of 23.4% (26/111) of the genomic DNA extracts were carriers of PCR-positive carbapenemase genes, including 14.4% blaNDM-1, 7.2% blaVIM-1 and 1.8% blaOXA-48. The highest prevalence of carbapenemase genes was from non-fermenters, Acinetobacter baumannii and Pseudomonas aeruginosa. For the ESBL genes tested, 96.4% (107/111) of the CR isolates co-harboured both TEM-1 and SHV-1 genes. The ERIC-PCR gel analysis exhibited 1 to 8 bands ranging from 50 to 800 bp. Band patterns of 93 complex dissimilarities were visually distinguished from the 111 CR isolates studied, while the remaining 18 showed band similarities in pairs.ConclusionOverall, ERIC-PCR fingerprints have shown a high level of diversity among the species of Gram-negative bacterial pathogens and specimen collection sites in this study. ERIC-PCR optimisation assays may serve as a suitable genotyping tool for the assessment of genetic diversity or close relatedness of isolates that are found in clinical settings.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique

et al. 2019

View full text Add to dashboard Cite

show abstract

“…All of the P. aeruginosa isolates were MDR and collected from several biological sources from patients admitted in different hospital units (18). The MIC 50 and MIC 90 of meropenem and ciprofloxacin for P. aeruginosa were 4 and 32 g/ml and 32 and 64 g/ml, respectively.…”

Section: Resultsmentioning

confidence: 99%

Structural Changes and Differentially Expressed Genes in Pseudomonas aeruginosa Exposed to Meropenem-Ciprofloxacin Combination

Siqueira

Cardoso

Caleffi-Ferracioli

et al. 2014

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

The effect of a meropenem-ciprofloxacin combination (MCC) on the susceptibility of multidrug-resistant (MDR) Pseudomonas aeruginosa (MRPA) clinical isolates was determined using checkerboard and time-kill curve techniques. Structural changes and differential gene expression that resulted from the synergistic action of the MCC against one of the P. aeruginosa isolates (1071-MRPA]) were evaluated using electron microscopy and representational difference analysis (RDA), respectively. The differentially expressed, SOS response-associated, and resistance-associated genes in 1071-MRPA exposed to meropenem, ciprofloxacin, and the MCC were monitored by quantitative PCR. The MCC was synergistic against 25% and 40.6% of MDR P. aeruginosa isolates as shown by the checkerboard and time-kill curves, respectively. The morphological and structural changes that resulted from the synergistic action of the MCC against 1071-MRPA were a summation of the effects observed with each antimicrobial alone. One exception included outer membrane vesicles, which were seen in a greater amount upon ciprofloxacin exposure but were significantly inhibited upon MCC exposure. Cell wall-and DNA repair-associated genes were differentially expressed in 1071-MRPA exposed to meropenem, ciprofloxacin, and the MCC. However, some of the RDA-detected, resistance-associated, and SOS response-associated genes were expressed at significantly lower levels in 1071-MRPA exposed to the MCC. The MCC may be an alternative for the treatment of MDR P. aeruginosa. The effect of this antimicrobial combination may be not only the result of a summation of the effects of meropenem and ciprofloxacin but also a result of differential action that likely inhibits protective mechanisms in the bacteria. Pseudomonas aeruginosa is a highly successful opportunistic pathogen that displays intrinsic multidrug resistance and has a great ability to acquire further resistance mechanisms (1, 2). Limited classes of antibiotics can be used for the treatment of P. aeruginosa infection (3). The emergence of multidrug-resistant (MDR) P. aeruginosa (MRPA) has prompted the search for new agents or alternative therapeutics (1, 4, 5), including antimicrobial combinations (6-9).Synergistic interactions between drugs have been investigated to improve therapeutic results, decrease the potential toxicity of antimicrobial agents, and prevent the emergence of bacterial resistance (6, 10, 11). Several studies have evaluated the effects of a meropenem-ciprofloxacin combination (MCC) in P. aeruginosa (12-14), including MDR isolates (8, 15). Despite some differences in the results, likely attributable to variations in techniques and bacterial isolates, the MCC represents a promising antipseudomonal therapeutic option (12)(13)(14). Although ␤-lactams and fluoroquinolones represent classic antimicrobials with previously known mechanisms of action, the molecular basis of synergism between these classes of drugs has not yet been elucidated.Thus, because of high rates of resistance observed in Brazilian P. ae...

show abstract

Evaluation of chromosomally and acquired mechanisms of resistance to carbapenem antibiotics among clinical isolates of Pseudomonas aeruginosa in Kerman, Iran

et al. 2020

View full text Add to dashboard Cite

High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil

Cited by 4 publications

References 30 publications

Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique

Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique

Structural Changes and Differentially Expressed Genes in Pseudomonas aeruginosa Exposed to Meropenem-Ciprofloxacin Combination

Evaluation of chromosomally and acquired mechanisms of resistance to carbapenem antibiotics among clinical isolates of Pseudomonas aeruginosa in Kerman, Iran

Contact Info

Product

Resources

About