Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

Abstract

Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).<br>Neste trabalho estudou-se a conversão da sacarose em frutose e ácido glicônico, usando as enzimas invertase, glicose oxidase e catalase, através do emprego de processo descontínuo (com adição sequencial ou simultânea das enzimas) e contínuo (adição simultânea das enzimas em reator com membrana acoplado à membrana de ultrafiltração de 100 kDa). Os parâmetros variados foram: a concentração das enzimas, a concentração inicial dos substratos (sacarose e glicose), o pH, a temperatura e a vazão específica de alimentação (processo contínuo). Obteve-se rendimento de 100%, quando a conversão foi conduzida por processo descontínuo em pH 4,5 e a 37 ºC com adição seqüencial das enzimas invertase (14,3 U), glicose oxidase (10.000 U) e catalase (59.000 U)

Improvement of Invertase Synthesis by the Mutant Saccharomyces cerevisiae through UV Mutagenesis

“…In different invertase production time duration experiment, the maximum enzyme activity was observed at 30 h (21.0 Units/ml) of incubation. Further increase in the incubation time significantly reduces the invertase activity its shown in Fig:5 Similar observation has been reported previously with pH 3.5-5.0 [35] and optimum temperature 70°C [36] observation also reported.…”

Comparative study of stability of soluble and cell wall invertase fromSaccharomyces cerevisiae

“…2). Similar pH optimum for soluble invertase was previously reported (35). Slight shift in pH optimum of soluble invertase and invertase immobilized on insoluble carriers is a common phenomenon described in previous research papers (9,36,37).…”

Enzyme production ofd-gluconic acid and glucose oxidase: successful tales of cascade reactions

“…This enzyme-powered device was successfully employed in Catalysis Science & Technology Mini review the autonomous and on-demand delivery of doxorubicin in HeLa cancer treatment. 307 Some studies reported the use of maltose and sucrose as substrates for D-gluconic acid production in batches using catalase to destroy the hydrogen peroxide 21,308,309 and using fed-batch, 310 continuous 311 and membrane-continuous 309,310 processes. In general, all processes were satisfactory, reaching high yields of conversion of maltose and sucrose to D-gluconic acid.…”