Molecular diversity in Coffea canephora germplasm conserved and cultivated in Brazil

Abstract

This work aimed to characterize accessions that represent the C. canephora germplasm conserved and cultivated in Brazil. A total of 130 accessions from germplasm banks of IAC (São Paulo), UFV (Minas Gerais) and also collected in plantations of the State of Espírito Santo and Rondônia were evaluated with a set of 20 new microsatellite primers. Multivariate methods were used to estimate the relationship among the accessions. High level of polymorphism and two major diversity clusters were identified. First cluster was composed by the accessions conserved in the IAC and UFV collections and the second was formed by accessions collected in areas under cultivation. Accessions from Espírito Santo and Rondônia were clear separated, composing two subclusters. Despite the great polymorphism found in Brazilian plantations, the diversity may be increased, because a new threshold in the genetic gains is expected on breeding programs with the intensification of the use of conserved germplasm.

Micronutrient Accumulation in Conilon Coffee Berries with Different Maturation Cycles

“…However, the difference between the super-late clone and the other genotypes confirms the great genetic diversity that exists among genotypes of the C. canephora species, as suggested by Souza et al (2013 (Figures 1, 2, and 3), are similar to results reported for C. arabica var. 'Caturra', for which the highest percentages of the accumulation of these nutrients were found between 90 and 120 days after anthesis (Ramírez et al, 2002).…”

Identification of microsatellite markers in coffee associated with resistance to Meloidogyne exigua

“…Similar results were reported by Souza et al (2013) using microsatellite markers that explained 24.9% of the total variation among the germplasm of C. canephora found in Brazil. In contrast to our findings, Setotaw et al (2010) were able to explain 67% of the total variation from the first two principal coordinates obtained using amplified fragment length polymorphism (AFLP) and random-amplified polymorphic DNA (RAPD) molecular markers.…”

Performance of intraspecific hybrids (Kouillou x Robusta) of Coffea canephora Pierre

“…During this period, the main objectives were the adaptation of cultivars and management techniques. Among those activities, there was an introduction of C. canephora progenies from the Agronomic Institute of Campinas -IAC, in São Paulo (Souza et al, 2013).…”