Genotoxic effect of Phenanthrene on Chironomus sancticaroli (Diptera: Chironomidae)

Morais, Gisele dos Santos; Pesenti, Emanuele Cristina; Cestari, Marta Margarete; Navarro-Silva, Mário Antônio

doi:10.1590/s1984-46702014000400003

Cited by 20 publications

(9 citation statements)

References 17 publications

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“…Experimental treatment of largemouth bass (Micropterus salmoides) displayed the neurotoxicity caused by phenanthrene (Martyniuk et al 2009). GT of this compound was demonstrated also for Chironomus sancticaroli larvae; even low phenanthrene concentrations induced formation of DNA strand-breaks (Comet assay) after acute and chronic exposure (dos Santos Morais et al 2014).…”

Section: Discussionmentioning

confidence: 90%

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Rybakovas

Arbačiauskas

Markovskienė

et al. 2019

Environ and Mol Mutagen

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European inland waters are under continuous threat of anthropogenic pollution. Determination of background level of biomarker response and subsequent classification of the impact increases the applicability of results. In the current study, we evaluate the range of chemical contamination by measuring the concentrations of metals, polycyclic aromatic hydrocarbons and polychlorinated biphenyls, and the levels of environmental genotoxicity by using the micronuclei and nuclear bud tests in bivalve mussels of the major Lithuanian rivers. Second, we aimed to evaluate the association between chemical contamination and genotoxicity biomarker responses. Finally, we set to determine the background level of genotoxic effects. Such value (summed frequency of MN and NB) was assessed—6‰. On that basis, we develop a scale of potential genotoxic impact and perform ranging sites into five categories. The results clearly indicate the existence of significant differences in the levels of chemical pollution and genotoxicity in different sites. Increased levels of studied parameters were assessed at the areas affected by municipal and industrial effluents, road runoff, combustion products, and in the area contaminated by accidental spillage. On the other hand, downstream decrease of contamination level, presumably associated with biological degradation and photochemical oxidation, were also observed. Genotoxicity parameters were associated with PAH and metal concentrations measured in mussel tissues as well as in sediments. Results also indicate that in situ genotoxicity assessment performed in the areas affected by long‐term contamination of municipal origin might be not sufficiently precise. Study highlights the necessity to combine genotoxicity assessment with chemical analysis. Environ. Mol. Mutagen. 61:338–354, 2020. © 2019 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 90%

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Rybakovas

Arbačiauskas

Markovskienė

et al. 2019

Environ and Mol Mutagen

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“…The level of removal of DBT from a solution of 1-10 mM DBT was determined by using a UV Sensor to visualize a curve with at a wavelength of 250 nm running through the metabolic liquid for different concentrations of DBT (0.5, 1.0, and 2.0 mM) at a constant temperature of 28°C and shaken at 150 rpm for 144 h. Measurements were made every 24 h [20].…”

Section: Determining the Removal Of Dbtmentioning

confidence: 99%

“…Analysis of the metabolites showed that C. elegans (UCP 596) can degrade DBT using two metabolic pathways. The literature describes three well-known pathways in the process of degrading DBT by microorganisms: Kodama; angular dioxygenation; and the sulfoxide, sulfone, sulfonate, sulfate pathway deemed the "4S" [6,9,[18][19][20]23]. Figure 4 shows the proposed routes of microbial degradation.…”

Section: Pathways For Biodegradation Of Dbt By C Elegansmentioning

confidence: 99%

“…The microorganisms that use this pathway to metabolize DBT manage to remove the potentially toxic atom of the thiophenic compound, in the form of a sulfate compound, with only a slight loss of energy value occurring [16,17,20,26]. Thus, the strains that use the "4S" pathway will be able to constitute a fundamental biological tool in the treatment, on a large scale, of fossil fuels, if biocatalysts in an industrial environment can be obtained [2,36,37].…”

Section: Pathways For Biodegradation Of Dbt By C Elegansmentioning

confidence: 99%

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Reduction in the Sulfur Content of Fossil Fuels by Cunninghamella elegans (UCP 0596) to Dibenzothiophene Compound

Souza¹,

Silva²,

Lima³

et al. 2018

Recent Insights in Petroleum Science and Engineering

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Biodesulfurization (BDS) is one of the most promising technologies used together with traditional hydrodesulfurization (HDS) to reduce the sulfur content of fossil fuels. In this research study, a strain of Cunninghamella elegans (UCP 596) was isolated from mangrove sediments to metabolize an organosulfur dibenzothiophene (DBT) compound in the concentrations of 0.5 and 1 mM and transform to DBT sulfone (DBT-5-dioxide), followed by dibenzothiophene 5,5-dioxide and 2-hydroxybiphenyl metabolites, thus suggesting the use of the "4S" metabolic pathway. The fungus also degraded the DBT completely in the first 24 h of growth on a 2.0 mM DBT concentration by angular deoxygenation, which suggests that a new second metabolic pathway was used. The DBT was consumed as the carbon source, and the sulfur was removed in the form of sulfite ion. A new product, benzoic acid, was formed at the end of the catabolism of DBT by C. elegans using an angular route.

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“…Owing to their robust physiology, midges have been a popular model system for the elucidation of cellular responses against different stressors e.g. heat, radiation, UV, toxicants and heavy metals (Choi and Ha 2009;Thorat and Nath 2010;Cao et al 2013;Morais et al 2014). In the past few decades, the use of midges for aquatic biomonitoring programmes has been increasingly evident (Rosenberg 1992).…”

Section: Introductionmentioning

confidence: 99%

Tolerance to desiccation stress in Chironomus Ramosus through plasticity in homeostatic control

Thorat

Nath

2015

European Journal of Environmental Sciences

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Desiccating environments pose physiological challenges to organisms, especially those experiencing them on recurring basis. Several desiccation-tolerant organisms are known to withstand loss of body water in response to seasonal dehydration patterns through adaptations that facilitate their sustenance in the dry state followed by recovery upon return of favourable hydrating conditions. In the present study, we have chosen Chironomus ramosus, an aquatic midge species as a laboratory model system towards the exploration of its desiccation tolerance ability. We confirm that upon desiccation exposure at low relative humidity, larvae of C. ramosus exhibited rapid water loss and could revive upon rehydration. Moreover, the revived individuals underwent successful metamorphosis; albeit delayed. This heterochrony in the developmental calendar was experimentally verified by the investigation of ecdysone levels which suggested an altered pattern of ecdysis in response to the stress. These data suggested the organism's plasticity in developmental homeostasis when confronted with dehydration stress. Furthermore, spectrofluorometric assays indicated the occurrence of thiol damage in the larvae. Investigation of Hsp70, the evolutionarily conserved stress responsive gene suggested that Hsp70 was indeed up-regulated during the rehydration period, thus facilitating recovery of the larvae from dehydration. In summary, several lines of evidence involving cellular, physiological and developmental adaptations revealed the ability of C. ramosus larvae to minimize the diverse categories of damage caused by desiccation stress through plasticity in their homeostatic mechanism.

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Genotoxic effect of Phenanthrene on Chironomus sancticaroli (Diptera: Chironomidae)

Cited by 20 publications

References 17 publications

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Reduction in the Sulfur Content of Fossil Fuels by Cunninghamella elegans (UCP 0596) to Dibenzothiophene Compound

Tolerance to desiccation stress in Chironomus Ramosus through plasticity in homeostatic control

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