Rodents as potential reservoirs for Borrelia spp. in northern Chile

Thomas, Richard; Santodomingo, Adriana; Muñoz-Léal, Sebastián; Fuente, María Carolina Silva-de la; Llanos-Soto, Sebastián; Moreno, Lucila; González‐Acuña, Daniel

doi:10.1590/s1984-29612020029

Cited by 16 publications

(11 citation statements)

References 40 publications

(48 reference statements)

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“…Despite this prevalence, OTUs analysis did not determine a preferred species for Borrelia readings, which could suggest the presence of a new species. Reports of Borrelia in ticks and wild mammals have been increasing in recent years in South America (Ataliba et al 2007 ; Kelly et al 2014 ; Muñoz-Leal et al 2018 , 2021b ; Morel et al 2019 ; Sánchez et al 2020 ), but with few records in Colombia (Marinkelle and Grose 1968 ; Muñoz-Leal et al 2021b ). Borrelia and Rickettsia have been the only confirmed potential pathogenic bacteria related to O. hasei (Tahir et al 2016 ; Colombo et al 2020 ; Muñoz-Leal et al 2021a ), however, all recorded pathogenic genera were detected in O .…”

Section: Discussionmentioning

confidence: 99%

Bacteria related to tick-borne pathogen assemblages in Ornithodoros cf. hasei (Acari: Argasidae) and blood of the wild mammal hosts in the Orinoquia region, Colombia

et al. 2022

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Interest in research on soft ticks has increased in recent decades, leading to valuable insight into their role as disease vectors. The use of metagenomics-based analyses have helped to elucidate ecological factors involved in pathogen, vector, and host dynamics. To understand the main bacterial assemblages present in Ornithodoros cf. hasei and its mammalian hosts, 84 ticks and 13 blood samples from bat hosts (Chiroptera) were selected, and the 16S rRNA gene V4 region was sequenced in five pools (each one related to each host-tick pairing). Bacterial taxonomic assignment analyses were performed by comparing operational taxonomic units (OTUs) shared between ticks and their host blood. This analysis showed the presence of Proteobacteria (38.8%), Enterobacteriaceae (25%), Firmicutes (12.3%), and Actinobacteria (10.9%) within blood samples, and Rickettsiaceae (39%), Firmicutes (25%), Actinobacteria (13.1%), and Proteobacteria (9%) within ticks. Species related to potentially pathogenic genera were detected in ticks, such as Borrelia sp., Bartonella tamiae, Ehrlichia sp. and Rickettsia-like endosymbiont, and the presence of these organisms was found in all analyzed bat species (Cynomops planirostris, Molossus pretiosus, Noctilio albiventris), and O. cf. hasei. About 41–48.6% of bacterial OTUs (genera and species) were shared between ticks and the blood of bat hosts. Targeted metagenomic screening techniques allowed the detection of tick-associated pathogens for O. cf. hasei and small mammals for the first time, enabling future research on many of these pathogens.

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Section: Discussionmentioning

confidence: 99%

Bacteria related to tick-borne pathogen assemblages in Ornithodoros cf. hasei (Acari: Argasidae) and blood of the wild mammal hosts in the Orinoquia region, Colombia

et al. 2022

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“…Otro estudio en el que no se logró identificar ADN de B. burgdorferi s.l. fue el realizado con M. musculus y R. rattus capturados en cinco localidades pertenecientes a regiones hidrográficas hiperáridas del norte de Chile por Sánchez y col. [29]. Estos autores señalan que el hecho de trabajar con un tamaño reducido de muestra (n = 3) condicionó sus resultados, factor que también pudo influir (aunque sea de forma parcial) en los hallazgos obtenidos en esta investigación (n = 82).…”

Section: Resultados Y Discusionunclassified

“…que contiene especies transmitidas principalmente por garrapatas de la familia Ixodidae y que ocasionan la borreliosis de Lyme [17,18]. Dentro de los ciclos enzoóticos de ambos grupos, los pequeños mamíferos, entre ellos los roedores, son relevantes por que pueden mantener a las bacterias en sus organismos y también porque son hospedadores accidentales de los artrópodos que transmiten a las especies de Borrelia [29,43].…”

Section: Introduccionunclassified

Frecuencia de Borrelia burgdorferi sensu lato y Leptospira spp. en pequeños roedores de Yucatán, México

et al. 2022

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Las bacterias espiroquetas Borrelia burgdorferi sensu lato (s.l.) y Leptospira spp. ocasionan la borreliosis de Lyme y leptospirosis, respectivamente, ambas enfermedades zoonóticas de importancia en salud pública. En sus respectivos ciclos epidemiológicos, los pequeños roedores son hospedadores accidentales y reservorios naturales de estas bacterias. Se capturaron pequeños roedores en las comunidades marginadas de Chan San Antonio y Sucopó, ubicadas en la zona oriente de Yucatán, México. Se tomaron muestras de vejiga, oreja y riñón y se utilizaron para la extracción del ácido desoxirribonucleico (ADN) genómico que se empleó para la búsqueda de ADN de B. burgdorferi s.l. y Leptospira spp. Se capturaron un total de 82 roedores de las especies Mus musculus, Rattus rattus y Heteromys gaumeri. En las muestras estudiadas no se encontró ADN de B. burgdorferi s.l. La infección con Leptospira spp. fue de 1,21 % (1/82). El único individuo positivo perteneció a la especie M. musculus. Se concluye que, si bien la frecuencia de infección con Leptospira spp. fue baja y que no se encontró evidencia de ADN de B. burgdorferi s.l. en la población estudiada de roedores, no puede descartarse su participación como hospederos accidentales o reservorios en los respectivos ciclos de transmisión de los géneros bacterianos evaluados.

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“…7. The bacterial burden is quantitated by qPCR by determining the flagellin gene flaB [14,15] (see Note 10).…”

Section: Peptides For Diagnostic Applicationsmentioning

confidence: 99%

Structure-Based Design of Diagnostics and Vaccines for Lyme Disease

Thomas

2021

Vaccine Design

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Changes in climate have increased the geographical range of insect vectors responsible for the transmission of several diseases. Lyme disease, caused by the bacterial pathogen Borrelia burgdorferi, has become recognized as the most prevalent arthropod-borne infection in the USA. It is transmitted to humans through the bite of infected blacklegged ticks. As yet, there are no commercial vaccines available that effectively provide protection against Lyme disease. Vaccination strategies involving use of subunit vaccines developed in many laboratories have been found to be less efficient in protecting against the disease. Hence, there is a need to develop powerful vaccines that provide robust protection against Borrelia. Recently, using the principle of structure-based design, we designed and developed novel diagnostics and vaccine candidates that protected against Lyme disease in animal models. This chapter describes design and development of peptides based on the principle of structure-based design for use in diagnostics and vaccines to protect against Lyme disease in an animal model.

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Rodents as potential reservoirs for Borrelia spp. in northern Chile

Cited by 16 publications

References 40 publications

Bacteria related to tick-borne pathogen assemblages in Ornithodoros cf. hasei (Acari: Argasidae) and blood of the wild mammal hosts in the Orinoquia region, Colombia

Bacteria related to tick-borne pathogen assemblages in Ornithodoros cf. hasei (Acari: Argasidae) and blood of the wild mammal hosts in the Orinoquia region, Colombia

Frecuencia de Borrelia burgdorferi sensu lato y Leptospira spp. en pequeños roedores de Yucatán, México

Structure-Based Design of Diagnostics and Vaccines for Lyme Disease

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