Evaluation of phytotherapy alternatives for controlling Rhipicephalus (Boophilus) microplus in vitro

Abstract

The objective of this study was to identify the main chemical components of the essential oil of Cuminum cyminum L. (cumin) and of the fixed oils of Bertholletia excelsa (Brazil nut) and of Helianthus annuus (sunflower seed). As well as testing the three oils and three different commercial synthetic acaricides against engorged females of Rhipicephalus (Boophilus) microplus in order to explore their acaricidal efficacy. Six different concentrations of the oils (200, 100, 50, 25, 12.5 and 6.25 mg/mL) and the active principles were evaluated with the Adult Immersion Test (AIT). The two main chemicals components of C. cyminum L. were the cuminaldehyde and the γ-terpinene. In both B. excelsa and H. annuus were the linoleic and oleic acid. C. cyminum L. showed high acaricidal activity (100%) over the engorged females and on their reproductive characteristat from the concentration of 100 mg/mL. B. excelsa and H. annuus had low acaricidal activity (39.39% and 58.75% in the concentration of 200 mg/mL respectively). The amidine and the pyrethroid (35.12% and 1.50% respectively). It can be concluded that the oil of C. cyminum L. may be a phytoterapic alternative for the cattle's tick control.

Efficacy of synergistic activity of seed oils from Carthamus tinctorius (Safflower) and Nasturtium officinale (Watercress) on lethality of the cattle tick Hyalomma scupense (Acari: Ixodidae)

“…(2021) investigated the effects of Mauritia flexuosa and Mauritiella armata seed oils on engorged females and larvae Rhipicephalus (Boophilus) microplus cattle tick; the in vitro tests revealed a typical reduction of the hatched larvae as well as inducing high larval mortality above 80% and reduction in the laying capacity of R. microplus at 5% and 10% concentrations. However, according to Villarreal et al . (2017) , the fixed oils of Bertholletia excelsa (Brazil nut) and Helianthus annuus (sunflower seed) had a low effect in vitro in cattle against engorged females of R. microplus , indicating that both fixed oils had low acaricidal activity (39.39% and 58.75%, respectively, at 200 mg/ml).…”

Óleos essenciais com atividade carrapaticida contra Rhipicephalus (Boophilus) microplus e Rhipicephalus sanguineus: uma revisão

“…(B.) microplus, o óleo de Cuminum cyminum mostrou alta atividade acaricida sobre fêmeas ingurgitadas, a partir da concentração 100 mg/mL, sendo o cuminaldeído e o γ-terpineno os compostos majoritários (Villarreal et al, 2017).…”

“…(B.) microplus, a citronela (Cymbopogon wynterianus), com óleo essencial rico em geraniol e citronelal, apresentou-se como carrapaticida a partir da concentração de 12,5%, sendo capaz de matar 100% das larvas e a 25% mostrou eficiência de 100% de mortalidade de fêmeas ingurgitadas (Santos et al, 2017;Clemente et al, 2010).…”

Óleos Essenciais com Atividade Acaricida para Controle de Rhipicephalus (Boophilus) microplus no Brasil

“…Diversos OEs das espécies exibidas neste estudo mostraram eficácia significativamente maior, quando em comparação com outros OEs testados, demonstrando 100% de letalidade contra R. microplus em um ou mais dos ensaios realizados (Tabela 1). São elas: Drimys brasiliensis (RIBEIRO et al, 2008), Tetradenia riparia (GAZIM et al, 2011), Lippia sidoides (GOMES et al, 2012), Tagetes minuta (GARCIA et al, 2012), Lippia triplinervis (LAGE et al, 2013), Syzygium aromaticum (MELLO et al, 2014), Zanthoxylum caribaeum (NOGUEIRA et al, 2014), Chrysopogon zizanioides (CAMPOS et al, 2015), Curcuma longa (CHAGAS et al, 2016), Zingiber officinale (CHAGAS et al, 2016), Lippia alba, L. gracilis, L. origanoides (CHAGAS et al, 2016), Cymbopogon martinii (PAZINATO et al, 2016), Cedrus atlântica (PAZINATO et al, 2016), C. citratus (PAZINATO et al, 2016;SILVA et al, 2020), Cuminum cyminum (VILLARREAL et al, 2017), Hesperozygis myrtoides (CASTILHO et al, 2017), Citrus limonum (VINTURELLE et al, 2017), Mesosphaerum suaveolens (CASTRO et al, 2018), Alpinia zerumbet (CASTRO et al, 2018), Croton conduplicatus, C. pulegiodoruse C. grewioides (CASTRO et al, 2019). Dos OEs utilizados, os três principais componentes majoritários identificados em maior concentração na análise quantitativa foram metil chavicol (95,38% e 83,59% em C. grewioides), sesquirosefurano (92,2% em C. grewioides) e geraniol (81,4% em C. martinii), nos respectivos estudos de Castro et al (2019), Figueiredo et al (2017) e Chagas et al (2012).…”