Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis

Simões, Aline Nunes; Almeida, Sayanne Luns Hatum de; Braga, Áquila Flávia da Rocha; Martins, Isabella Vilhena Freire; Donatele, Dirlei Molinari; Barioni, Graziela

doi:10.1590/s1984-29612017016

Cited by 2 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IDEXX kit (f2 antigen) (Table 1) (originally the Pourquier ELISA) has shown to be very reliable, as previous experimental infections reported sensitivity and specificity ratios of 100% [38, 41] and close to a 100% in natural infections [42, 43]. However, a study by Simões et al in 2017 reported a specificity ratio of 56% in Brazil [44]. The E/S antigen, in the Svanovir kit (Table 1), has previously shown a strong correlation between F. hepatica antibody levels, intra-hepatic fluke frequency and production parameters [11, 45] and a sensitivity and specificity of 92 and 88%, respectively, in BTM samples compared to sera [30].…”

Section: Discussionmentioning

confidence: 99%

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle

et al. 2019

View full text Add to dashboard Cite

BackgroundFasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate.ResultsIn the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied.ConclusionsSe and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle

et al. 2019

View full text Add to dashboard Cite

show abstract

Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

et al. 2023

View full text Add to dashboard Cite

Background and Aim: Bovine fasciolosis is a reemerging neglected disease with a worldwide distribution caused by the trematode Fasciola spp., which parasitize various hosts. Bovine fasciolosis is responsible for large economic losses in the bovine livestock sector. This study aimed to estimate the seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia. Materials and Methods: This was a descriptive cross-sectional study with simple random sampling conducted on 1140 cattle from the municipalities of Chiquinquir´, San Miguel de Sema, and Ubaté for a duration of 3 months. Serum samples were processed using the commercial Fasciola hepatica Antibody Test Kit IDEXX® Fasciolosis Verification (IDEXX, United States), which identified immunoglobulin G antibodies for gf2 antigen purified from Fasciola extracts. The f2 antigen is extremely immunogenic and highly specific for F. hepatica. An epidemiological survey was performed to record variables related to the sampled animals and herd management practices. Data were processed using the statistical program Epi Info® (Centers for Disease Control and Prevention; Atlanta, Georgia). The prevalence ratio was estimated to evaluate the association between fasciolosis and the hypothesized causal factors and the significance of this association using Pearson’s Chi-square test. Finally, a logistic regression model was developed. Results: The overall seroprevalence was 72.3%. The seroprevalence was 83.9% (323/385) in Chiquinquirá, 68.17% (257/377) in Ubaté, and 64.55% (244/378) in San Miguel de Sema. The seroprevalence was higher in male animals in Chiquinquirá and in female animals in San Miguel de Sema and Ubaté. Similarly, sex showed a statistically significant association with disease prevalence in Ubaté. The highest prevalence was found in cattle aged >2 years. The Holstein breed showed maximum seroprevalence in Chiquinquirá (p ≤ 0.05) and San Miguel de Sema, whereas crossbreed showed higher seroprevalence in Ubaté. Similarly, in Chiquinquirá, the association between the seroprevalence of fasciolosis and the presence of other species was statistically significant (95% confidence interval [CI]: 0.9601–3.4944; p = 0.0448). In Ubaté, the disease presentation was also associated with pasture rental (95% CI: 0.4047–1.0023; p = 0.003) and attendance to livestock expositions (95% CI: 0.2313–1.0636; p = 0.044). However, in San Miguel de Sema, water from the stream showed a statistically significant association with disease presentation (95% CI: 0.5209–1.0985; p = 0.00649785). Female sex and diarrhea occurrence were considered risk factors for fasciolosis. Conclusion: A high seroprevalence of antibodies to Fasciola spp. was detected in cattle in the study municipalities, indicating a high parasite distribution in these areas. Female sex and diarrhea were established as risk factors associated with fasciolosis in Ubaté and San Miguel de Sema, respectively. Further, research is necessary to establish prevention and control programs against parasitosis. Keywords: bovine, fasciolosis, risk factors, seroprevalence.

show abstract

Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis

Cited by 2 publications

References 9 publications

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle

Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

Contact Info

Product

Resources

About