Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Gaínza, Yousmel Alemán; Fantatto, Rafaela Regina; Chaves, F. C. M.; Bizzo, H. R.; Esteves, S. N.; Chagas, A. C. de S.

doi:10.1590/s1984-29612016073

Cited by 22 publications

(10 citation statements)

References 58 publications

(52 reference statements)

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“…The Piper genus constitutes the most represented, with around 2000 species [ 3 ]. In particular, biological properties of essential oils (EOs) from Piper aduncum L. ( Figure 1 ) have been widely studied, including antiprotozoal [ 4 , 5 ], anthelminthic [ 6 ], antioxidant [ 7 ], and insecticidal [ 8 , 9 ] activities. Considering these previously documented effects and the potentialities as therapeutic agents, we have focused on the EO from P. aduncum growing in Cuba.…”

Section: Introductionmentioning

confidence: 99%

Essential Oil from Piper aduncum: Chemical Analysis, Antimicrobial Assessment, and Literature Review

et al. 2017

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Background: The challenge in antimicrobial chemotherapy is to find safe and selective agents with potency that will not be compromised by previously developed resistance. Terrestrial plants could provide new leads to antibacterial, antifungal, or antiprotozoal activity. Methods: The essential oil (EO) of Piper aduncum L. (Piperaceae) from Cuba was analyzed by gas chromatography—mass spectrometry (GC-MS). A cluster analysis of P. aduncum EO compositions reported in the literature was carried out. The EO was screened against a panel of microorganisms (bacteria, fungi, parasitic protozoa) as well as for cytotoxicity against human cells. In addition, a review of scientific literature and a bibliometric study was also conducted. Results: A total of 90 compounds were identified in the EO, of which camphor (17.1%), viridiflorol (14.5%), and piperitone (23.7%) were the main components. The cluster analysis revealed at least nine different chemotypes. The EO did not show notable activity against bacteria or fungi, but was active against parasitic protozoa. Conclusions: The results from this study indicate P. aduncum from Cuba is a unique chemotype, support the importance of P. aduncum EOs as medicines, and demonstrate the promise of Cuban P. aduncum EO as a chemotherapeutic agent against parasitic protozoal infections.

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Section: Introductionmentioning

confidence: 99%

Essential Oil from Piper aduncum: Chemical Analysis, Antimicrobial Assessment, and Literature Review

et al. 2017

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“…Estudos que avaliaram o efeito do OE de Eucalyptus staigeriana sobre NGI identificaram o limoneno [62,84] ou o geranial [82,83] como compostos majo-ritários. Já o OE de Piper aduncum quando avaliado sobre H. contortus apresentou como composto majoritário o eucaliptol [71] ou o dilapiol [25]. A caracterização dos quimiotipos dos OE (caracterização dos genótipos das plantas utilizadas para extração dos OE de acordo com seus componentes majoritários e origem geográfica) [49] pode ser uma estratégia a ser adotada para padronização dos estudos que avaliam a ação anti-helmíntica.…”

Section: óLeos Essenciaisunclassified

“…Os testes in vitro devem ser realizados com populações de NGI com nível de resistência anti--helmíntica conhecida, pois as concentrações efetivas dos compostos bioativos sobre isolados de H. contortus multirresistentes podem ser superiores às concentrações efetivas dos compostos bioativos sobre isolados sensíveis. A utilização de OE para o controle de H. contortus sensível e multirresistente a anti-helmínticos sintéticos indica que os isolados resistentes são naturalmente capazes de tolerar os metabolitos secundários das plantas [13,14,25]. A avaliação da tolerância dos nematoides aos produtos naturais pode ser baseada no cálculo da razão de resistência (RR) de cada isolado de H. contortus resistente em relação ao isolado sensível, a partir dos valores de CE 50 (capacidade de inibir em 50%) obtido nos testes in vitro sobre ovos e larvas [13,14].…”

Section: Efeito Anti-helmíntico De óLeos Essenciaisunclassified

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

André

Ribeiro

Oliveira

et al. 2018

Acta Scientiae. Vet.

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Background: Gastrointestinal nematodes are one of the major health and economic problem of sheep and goats in the world. The control of these nematodes is carried out conventionally with synthetic anthelminths, which favored the selection of gastrointestinal nematode (GIN) populations multiresistant to anthelmintics. The emergence of anthelmintic resistance has stimulated the search for new alternatives to control small ruminant GIN, standing out the use of plants and their bioactives compounds, such as essential oils (EO). The objective of this review was to present the main characteristics and anthelmintic activity of EO, their isolated compounds and drug delivery systems in the control of GIN.Review: Essential oils are a complex blend of bioactive compounds with volatile, lipophilic, usually odoriferous and liquid substances. EO are composed of terpenes, terpenoids, aromatic and aliphatic constituents. EO has various pharmacological activities of interest in preventive veterinary medicine such as antibacterials, antifungals, anticoccicids, insecticides and anthelmintics. In vitro and in vivo tests are used to validate the anthelmintic activity of EO on GIN. In vitro tests are low cost screening tests that allow the evaluation of the anthelmintic activity of a large amount of bioactive compounds on eggs, first (L1) and third stage larvae (L3), and adult nematodes. The antiparasitic effect of EO is related to its main compound or to the interaction of the compounds. These bioactive compounds penetrate the cuticle of the nematodes by transcuticular diffusion, altering the mechanisms of locomotion, besides causing cuticular lesions. Following in vitro evaluation, the acute and sub-chronic toxicity test should be performed to assess the toxicity of the bioactive compounds and to define the dose to be used in in vivo tests. In vivo tests are more reliable because the anthelmintic effectiveness of bioactive compounds is evaluated after the metabolization process. The metabolization process of the bioactive compounds can generate metabolites that exhibit or not anthelmintic effectiveness. The in vivo tests assessing the anthelmintic effectiveness of bioactive compounds in sheep and goats are the fecal egg count reduction test and the controlled test. OE promoted reduction of egg elimination in faeces which may be related to cuticular and reproductive alterations in GIN, and reduction of parasite burden in in vivo tests. Due to the promising results obtained with OE in the in vivo tests, interest has been aroused in using nanotechnology as an alternative to increase the bioavailability of OE and consequently, potentializing its anthelmintic effect, reducing the dose and toxicity of the biocompounds. In addition to nanotechnology, the isolation and chemical modification of compounds isolated from OE have been employed to obtain new molecules with anthelmintic action and understand the mechanism of action of EO on the small ruminant GIN.Conclusion: The use of EO and their compound bioactive in the control of resistant populations of GIN is a promising alternative. The adoption of strategies in which natural products can replace synthetic anthelmintics, such as in dry periods and use synthetic anthelmintics in the rainy season when the population in refugia in the pasture is high, thus reducing the dissemination of GIN resistant populations. As perspective, the evaluation of pharmacokinetics and pharmacodynamics of these natural products should be performed so that one defines treatment protocols that optimize the anthelmintic effect.

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“…Piper aduncum L. (Piperaceae) is a plant native to the Amazon region that stands out for its therapeutic action against fungi, stomach ache, rheumatism, fever and general infection (Mgbeahuruike et al 2017), protozoan parasites (Dal Picolo et al 2014), and for its repellent properties (Mamood et al 2017). The chemical characterization of the metabolites present in P. aduncum reveals that the major compound is dillapiole (Gaínza et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Ecological risk assessment of Piper aduncum essential oil in non-target organisms

et al. 2021

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One possible alternative to chemotherapeutic agents in the treatment and prevention of diseases in fish farms is the use of Piper aduncum essential oil. However, ecotoxicological data are required to ensure its proper use and to prevent adverse effects on non-target organisms. These data are relevant since this essential oil is described as having insecticidal, molluscicidal and cytotoxic activitiy that may be associated with its chemical composition. Thus, the aim of this study was to evaluate the ecotoxicity of P. aduncum essential oil to five test organisms using the species sensitivity distribution (SSD) statistical approach. The chemical composition of the essential oil was characterized by means of gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) for identification and quantitation purposes, respectively. The main component (75.5%) of the essential oil was dillapiole. The hazardous concentration for 5% of biological species (HC5) was calculated to determine the 95% protection level, resulting in a value of 0.47 mg L-1 (with a confidence interval of 0.028 - 1.19 mg L-1. ). A concentration range related to the level of protection for aquatic communities (the predicted no-effect concentration, PNEC) was determined through the application of safety factors to the HC5 value. The ecotoxicity parameters showed that P. aduncum essential oil can be used safely in water bodies at a concentration equal to or below 0.09 mg L-1.

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Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Cited by 22 publications

References 58 publications

Essential Oil from Piper aduncum: Chemical Analysis, Antimicrobial Assessment, and Literature Review

Essential Oil from Piper aduncum: Chemical Analysis, Antimicrobial Assessment, and Literature Review

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

Ecological risk assessment of Piper aduncum essential oil in non-target organisms

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