Antifungal activity of Stenotrophomonas maltophilia in Stomoxys calcitranslarvae

Moraes, Ana Paula Rodrigues; Videira, Sandy Sampaio; Bittencourt, Vânia Rita Elias Pinheiro; Bittencourt, Avelino José

doi:10.1590/s1984-29612014037

Cited by 17 publications

(10 citation statements)

References 27 publications

(26 reference statements)

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“…bipustulata laboratory samples. A bacterium of the genus Stenotrophomonas has been recently described to protect its host, the fly Stomoxys calcitrans , against Beauveria bassiana an entomopathogenic fungus [ 92 ]. These bacteria are described as environmental bacteria present in water and soils but they are also described as opportunistic gut bacteria that can invade insect guts and prevent the invasion by other pathogenic bacteria [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community Diversity Harboured by Interacting Species

et al. 2016

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All animals are infected by microbial partners that can be passengers or residents and influence many biological traits of their hosts. Even if important factors that structure the composition and abundance of microbial communities within and among host individuals have been recently described, such as diet, developmental stage or phylogeny, few studies have conducted cross-taxonomic comparisons, especially on host species related by trophic relationships. Here, we describe and compare the microbial communities associated with the cabbage root fly Delia radicum and its three major parasitoids: the two staphylinid beetles Aleochara bilineata and A. bipustulata and the hymenopteran parasitoid Trybliographa rapae. For each species, two populations from Western France were sampled and microbial communities were described through culture independent methods (454 pyrosequencing). Each sample harbored at least 59 to 261 different bacterial phylotypes but was strongly dominated by one or two. Microbial communities differed markedly in terms of composition and abundance, being mainly influenced by phylogenetic proximity but also geography to a minor extent. Surprisingly, despite their strong trophic interaction, parasitoids shared a very low proportion of microbial partners with their insect host. Three vertically transmitted symbionts from the genus Wolbachia, Rickettsia, and Spiroplasma were found in this study. Among them, Wolbachia and Spiroplasma were found in both the cabbage fly and at least one of its parasitoids, which could result from horizontal transfers through trophic interactions. Phylogenetic analysis showed that this hypothesis may explain some but not all cases. More work is needed to understand the dynamics of symbiotic associations within trophic network and the effect of these bacterial communities on the fitness of their hosts.

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Section: Discussionmentioning

confidence: 99%

Bacterial Community Diversity Harboured by Interacting Species

et al. 2016

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“…However, this species resides in a broad range of environments and are commonly identi ed only as multidrug-resistant opportunistic pathogens of humans [47], in soils or in association with plants [48], and also associated with multiple insect species, including the diamondback moth Plutella xylostella [49], the red turpentine beetle Dendroctonus valens [50], the twelve-toothed pine bark beetle Ips sexdentatus [51], the asian malaria mosquito Anopheles stephensi [52], the peach fruit y Bactrocera zonata [53], the muga silkworm Antheraea assamensis [54] and the wings of the Colorado potato beetle Leptinotarsa decemlineata [55]. S. maltophilia was also isolated from the honeydew of Indian lac insect Kerria lacca [56] and from the stable y Stomoxys calcitrans larvae presenting antifungal activity against Beauveria bassiana [57]. Furthermore, S. maltophilia associated with Delia antiqua larvae can inhibit B. bassiana infection [58].…”

Section: Discussionmentioning

confidence: 99%

Immune Response and Hemolymph Microbiota ofApis melliferaandApis ceranaAfter the Challenge With RecombinantVarroaToxic Protein

Balakrishnan

Cao

et al. 2021

Journal of Economic Entomology

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The honey bee is a significant crop pollinator and key model insect for understanding social behavior, disease transmission, and development. The ectoparasitic Varroa destructor mite put threats on the honey bee industry. A Varroa toxic protein (VTP) from the saliva of Varroa mites contributes to the toxicity toward Apis cerana and the deformed wing virus elevation in Apis mellifera. However, the immune response and hemolymph microbiota of honey bee species after the injection of recombinant VTP has not yet been reported. In this study, both A. cerana and A. mellifera worker larvae were injected with the recombinant VTP. Then the expressions of the honey bee immune genes abaecin, defensin, and domeless at three time points were determined by qRT–PCR, and hemolymph microbial community were analyzed by culture-dependent method, after recombinant VTP injection. The mortality rates of A. cerana larvae were much higher than those of A. mellifera larvae after VTP challenge. VTP injection induced the upregulation of defensin gene expression in A. mellifera larvae, and higher levels of abaecin and domeless mRNAs response in A. cerana larvae, compared with the control (without any injection). Phosphate buffer saline (PBS) injection also upregulated the expression levels of abaecin, defensin, and domeless in A. mellifera and A. cerana larvae. Three bacterial species (Enterococcus faecalis, Staphylococcus cohnii, and Bacillus cereus) were isolated from the hemolymph of A. cerana larvae after VTP injection and at 48 h after PBS injections. Two bacterial species (Stenotrophomonas maltophilia and Staphylococcus aureus) were isolated from A. mellifera larvae after VTP challenge. No bacterial colonies were detected from the larval hemolymph of both honey bee species treated by injection only and the control. The result indicates that abaecin, defensin, and domeless genes and hemolymph microbiota respond to the VTP challenge. VTP injection might induce the dramatic growth of different bacterial species in the hemolymph of the injected larvae of A. mellifera and A. cerana, which provide cues for further studying the interactions among the honey bee, VTP, and hemolymph bacteria.

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“…López-Sánchez et al, 2012;Cruz-Vazquez et al, 2015;Baleba et al, 2021). Even, antifungal activity of S. calcitrans larvae against the fungi was documented (Moraes et al, 2014). Therefore, adult flies were included only in the current research.…”

Section: Pathogenicity Of Entomopathogenic Fungi To Stable Fly Adults...mentioning

confidence: 99%

Entomopathogenic fungi associated with Stomoxys calcitrans in Slovakia and efficacy of local fungal strains against the stable fly

Barta

2022

Acta fytotechn zootechn

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The stable fly, Stomoxys calcitrans, is the most important ectoparasite of livestock throughout the world. Entomopathogenic fungi are natural antagonists of stable flies with the potential to be used as biocontrol agents in integrated management programmes of this pest. The prevalence and species diversity of entomopathogenic fungi in stable fly populations were documented on a dairy cattle farm in south-western Slovakia. The prevalence of fungal infection was low and the maximum percentage of infected flies reached 2.72% during May-October in 2016. Three Beauveria species were identified from infected flies, B. bassiana, B. pseudobassiana and B. brongniartii. Beauveria pseudobassiana and B. brongniartii are reported from S. calcitrans for the first time. In laboratory tests, the pathogenicity of four entomopathogenic fungi B. bassiana, B. pseudobassiana, M. anisopliae and Isaria fumosorosea against adult stable flies was compared. The flies were susceptible to infection by the fungi and the most virulent isolate of B. bassiana (IKEP10) was selected for spore formulation testing and field trials. We tested three types of spore formulations and the aqueous spore suspension was more effective against stable flies than spores formulated in the powder or oil suspension. Repeated treatments of the interior of the stable with B. bassiana spores at fortnightly intervals during June-August resulted in a 10 times higher prevalence of fungal infection in the stable fly population compared with the untreated stable, but the treatments did not effectively reduce the fly population. The selected B. bassiana isolate may represent an interesting tool in the biocontrol of stable fly populations, but further experiments are needed to design an appropriate formulation and enhance its efficacy.

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Antifungal activity of Stenotrophomonas maltophilia in Stomoxys calcitranslarvae

Cited by 17 publications

References 27 publications

Bacterial Community Diversity Harboured by Interacting Species

Bacterial Community Diversity Harboured by Interacting Species

Immune Response and Hemolymph Microbiota ofApis melliferaandApis ceranaAfter the Challenge With RecombinantVarroaToxic Protein

Entomopathogenic fungi associated with Stomoxys calcitrans in Slovakia and efficacy of local fungal strains against the stable fly

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